The PPE domain of PPE17 is responsible for its surface localization and can be used to express heterologous proteins on the mycobacterial surface.

PPE represent a peculiar family of mycobacterial proteins characterized by a 180 aminoacids conserved N-terminal domain. Several PPE genes are co-transcribed with a gene encoding for a protein belonging to another family of mycobacterial specific proteins named PE. Only one PE-PPE couple has been ex...

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Autores principales:	Valentina Donà, Marcello Ventura, Michela Sali, Alessandro Cascioferro, Roberta Provvedi, Giorgio Palù, Giovanni Delogu, Riccardo Manganelli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3d300b74b39d4fe797a8ebe99f292251
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spelling	oai:doaj.org-article:3d300b74b39d4fe797a8ebe99f2922512021-11-18T07:55:14ZThe PPE domain of PPE17 is responsible for its surface localization and can be used to express heterologous proteins on the mycobacterial surface.1932-620310.1371/journal.pone.0057517https://doaj.org/article/3d300b74b39d4fe797a8ebe99f2922512013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23469198/?tool=EBIhttps://doaj.org/toc/1932-6203PPE represent a peculiar family of mycobacterial proteins characterized by a 180 aminoacids conserved N-terminal domain. Several PPE genes are co-transcribed with a gene encoding for a protein belonging to another family of mycobacterial specific proteins named PE. Only one PE-PPE couple has been extensively characterized so far (PE25-PPE41) and it was shown that these two proteins form a heterodimer and that this interaction is essential for PPE41 stability and translocation through the mycobacterial cell wall. In this study we characterize the PE11-PPE17 couple. In contrast with what was found for PE25-PPE41, we show that PPE17 is not secreted but surface exposed. Moreover, we demonstrate that the presence of PE11 is not necessary for PPE17 stability or for its localization on the mycobacterial surface. Finally, we show that the PPE domain of PPE17 targets the mycobacterial cell wall and that this domain can be used as a fusion partner to expose heterologous proteins on the mycobacterial surface.Valentina DonàMarcello VenturaMichela SaliAlessandro CascioferroRoberta ProvvediGiorgio PalùGiovanni DeloguRiccardo ManganelliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e57517 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valentina Donà Marcello Ventura Michela Sali Alessandro Cascioferro Roberta Provvedi Giorgio Palù Giovanni Delogu Riccardo Manganelli The PPE domain of PPE17 is responsible for its surface localization and can be used to express heterologous proteins on the mycobacterial surface.
description	PPE represent a peculiar family of mycobacterial proteins characterized by a 180 aminoacids conserved N-terminal domain. Several PPE genes are co-transcribed with a gene encoding for a protein belonging to another family of mycobacterial specific proteins named PE. Only one PE-PPE couple has been extensively characterized so far (PE25-PPE41) and it was shown that these two proteins form a heterodimer and that this interaction is essential for PPE41 stability and translocation through the mycobacterial cell wall. In this study we characterize the PE11-PPE17 couple. In contrast with what was found for PE25-PPE41, we show that PPE17 is not secreted but surface exposed. Moreover, we demonstrate that the presence of PE11 is not necessary for PPE17 stability or for its localization on the mycobacterial surface. Finally, we show that the PPE domain of PPE17 targets the mycobacterial cell wall and that this domain can be used as a fusion partner to expose heterologous proteins on the mycobacterial surface.
format	article
author	Valentina Donà Marcello Ventura Michela Sali Alessandro Cascioferro Roberta Provvedi Giorgio Palù Giovanni Delogu Riccardo Manganelli
author_facet	Valentina Donà Marcello Ventura Michela Sali Alessandro Cascioferro Roberta Provvedi Giorgio Palù Giovanni Delogu Riccardo Manganelli
author_sort	Valentina Donà
title	The PPE domain of PPE17 is responsible for its surface localization and can be used to express heterologous proteins on the mycobacterial surface.
title_short	The PPE domain of PPE17 is responsible for its surface localization and can be used to express heterologous proteins on the mycobacterial surface.
title_full	The PPE domain of PPE17 is responsible for its surface localization and can be used to express heterologous proteins on the mycobacterial surface.
title_fullStr	The PPE domain of PPE17 is responsible for its surface localization and can be used to express heterologous proteins on the mycobacterial surface.
title_full_unstemmed	The PPE domain of PPE17 is responsible for its surface localization and can be used to express heterologous proteins on the mycobacterial surface.
title_sort	ppe domain of ppe17 is responsible for its surface localization and can be used to express heterologous proteins on the mycobacterial surface.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3d300b74b39d4fe797a8ebe99f292251
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The PPE domain of PPE17 is responsible for its surface localization and can be used to express heterologous proteins on the mycobacterial surface.

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