Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling

Abstract Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due to a lack of sufficient methods to examine these parameters in vivo at high resolution throughout an angiogenic network. The availability of surrogate markers for functional vascu...

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Autores principales:	Molly R. Kelly-Goss, Bo Ning, Anthony C. Bruce, Daniel N. Tavakol, David Yi, Song Hu, Paul A. Yates, Shayn M. Peirce
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3d36348c06904de49dd48b0d52c4e846
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spelling	oai:doaj.org-article:3d36348c06904de49dd48b0d52c4e8462021-12-02T16:06:24ZDynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling10.1038/s41598-017-08982-z2045-2322https://doaj.org/article/3d36348c06904de49dd48b0d52c4e8462017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08982-zhttps://doaj.org/toc/2045-2322Abstract Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due to a lack of sufficient methods to examine these parameters in vivo at high resolution throughout an angiogenic network. The availability of surrogate markers for functional vascular proteins, such as green fluorescent protein, enables expression in individual cells to be followed over time using confocal microscopy, while photoacoustic microscopy enables dynamic measurement of blood flow across the network with capillary-level resolution. We combined these two non-invasive imaging modalities in order to spatially and temporally analyze biochemical and biomechanical drivers of angiogenesis in murine corneal neovessels. By stimulating corneal angiogenesis with an alkali burn in Tie2-GFP fluorescent-reporter mice, we evaluated how onset of blood flow and surgically-altered blood flow affects Tie2-GFP expression. Our study establishes a novel platform for analyzing heterogeneous blood flow and fluorescent reporter protein expression across a dynamic microvascular network in an adult mammal.Molly R. Kelly-GossBo NingAnthony C. BruceDaniel N. TavakolDavid YiSong HuPaul A. YatesShayn M. PeirceNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Molly R. Kelly-Goss Bo Ning Anthony C. Bruce Daniel N. Tavakol David Yi Song Hu Paul A. Yates Shayn M. Peirce Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
description	Abstract Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due to a lack of sufficient methods to examine these parameters in vivo at high resolution throughout an angiogenic network. The availability of surrogate markers for functional vascular proteins, such as green fluorescent protein, enables expression in individual cells to be followed over time using confocal microscopy, while photoacoustic microscopy enables dynamic measurement of blood flow across the network with capillary-level resolution. We combined these two non-invasive imaging modalities in order to spatially and temporally analyze biochemical and biomechanical drivers of angiogenesis in murine corneal neovessels. By stimulating corneal angiogenesis with an alkali burn in Tie2-GFP fluorescent-reporter mice, we evaluated how onset of blood flow and surgically-altered blood flow affects Tie2-GFP expression. Our study establishes a novel platform for analyzing heterogeneous blood flow and fluorescent reporter protein expression across a dynamic microvascular network in an adult mammal.
format	article
author	Molly R. Kelly-Goss Bo Ning Anthony C. Bruce Daniel N. Tavakol David Yi Song Hu Paul A. Yates Shayn M. Peirce
author_facet	Molly R. Kelly-Goss Bo Ning Anthony C. Bruce Daniel N. Tavakol David Yi Song Hu Paul A. Yates Shayn M. Peirce
author_sort	Molly R. Kelly-Goss
title	Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
title_short	Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
title_full	Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
title_fullStr	Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
title_full_unstemmed	Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling
title_sort	dynamic, heterogeneous endothelial tie2 expression and capillary blood flow during microvascular remodeling
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3d36348c06904de49dd48b0d52c4e846
work_keys_str_mv	AT mollyrkellygoss dynamicheterogeneousendothelialtie2expressionandcapillarybloodflowduringmicrovascularremodeling AT boning dynamicheterogeneousendothelialtie2expressionandcapillarybloodflowduringmicrovascularremodeling AT anthonycbruce dynamicheterogeneousendothelialtie2expressionandcapillarybloodflowduringmicrovascularremodeling AT danielntavakol dynamicheterogeneousendothelialtie2expressionandcapillarybloodflowduringmicrovascularremodeling AT davidyi dynamicheterogeneousendothelialtie2expressionandcapillarybloodflowduringmicrovascularremodeling AT songhu dynamicheterogeneousendothelialtie2expressionandcapillarybloodflowduringmicrovascularremodeling AT paulayates dynamicheterogeneousendothelialtie2expressionandcapillarybloodflowduringmicrovascularremodeling AT shaynmpeirce dynamicheterogeneousendothelialtie2expressionandcapillarybloodflowduringmicrovascularremodeling
_version_	1718385017354190848

Dynamic, heterogeneous endothelial Tie2 expression and capillary blood flow during microvascular remodeling

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