Transcriptome of the Krushinsky-Molodkina Audiogenic Rat Strain and Identification of Possible Audiogenic Epilepsy-Associated Genes

Audiogenic epilepsy (AE), inherent to several rodent strains is widely studied as a model of generalized convulsive epilepsy. The molecular mechanisms that determine the manifestation of AE are not well understood. In the present work, we compared transcriptomes from the corpora quadrigemina in the...

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Autores principales:	Lyubov N. Chuvakova, Sergei Yu Funikov, Alexander P. Rezvykh, Artem I. Davletshin, Michael B. Evgen’ev, Svetlana A. Litvinova, Irina B. Fedotova, Inga I. Poletaeva, David G. Garbuz
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	audiogenic epilepsy Wistar rats KM rat strain transcriptomic analysis (RNA-seq) Ttr Msh3 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/3d398a569e2443a78575af68c3f6c41c
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spelling	oai:doaj.org-article:3d398a569e2443a78575af68c3f6c41c2021-11-04T08:53:39ZTranscriptome of the Krushinsky-Molodkina Audiogenic Rat Strain and Identification of Possible Audiogenic Epilepsy-Associated Genes1662-509910.3389/fnmol.2021.738930https://doaj.org/article/3d398a569e2443a78575af68c3f6c41c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnmol.2021.738930/fullhttps://doaj.org/toc/1662-5099Audiogenic epilepsy (AE), inherent to several rodent strains is widely studied as a model of generalized convulsive epilepsy. The molecular mechanisms that determine the manifestation of AE are not well understood. In the present work, we compared transcriptomes from the corpora quadrigemina in the midbrain zone, which are crucial for AE development, to identify genes associated with the AE phenotype. Three rat strains without sound exposure were compared: Krushinsky-Molodkina (KM) strain (100% AE-prone); Wistar outbred rat strain (non-AE prone) and “0” strain (partially AE-prone), selected from F2 KM × Wistar hybrids for their lack of AE. The findings showed that the KM strain gene expression profile exhibited a number of characteristics that differed from those of the Wistar and “0” strain profiles. In particular, the KM rats showed increased expression of a number of genes involved in the positive regulation of the MAPK signaling cascade and genes involved in the positive regulation of apoptotic processes. Another characteristic of the KM strain which differed from that of the Wistar and “0” rats was a multi-fold increase in the expression level of the Ttr gene and a significant decrease in the expression of the Msh3 gene. Decreased expression of a number of oxidative phosphorylation-related genes and a few other genes was also identified in the KM strain. Our data confirm the complex multigenic nature of AE inheritance in rodents. A comparison with data obtained from other independently selected AE-prone rodent strains suggests some common causes for the formation of the audiogenic phenotype.Lyubov N. ChuvakovaSergei Yu FunikovAlexander P. RezvykhAlexander P. RezvykhArtem I. DavletshinMichael B. Evgen’evSvetlana A. LitvinovaIrina B. FedotovaInga I. PoletaevaDavid G. GarbuzFrontiers Media S.A.articleaudiogenic epilepsyWistar ratsKM rat straintranscriptomic analysis (RNA-seq)TtrMsh3Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Molecular Neuroscience, Vol 14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	audiogenic epilepsy Wistar rats KM rat strain transcriptomic analysis (RNA-seq) Ttr Msh3 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	audiogenic epilepsy Wistar rats KM rat strain transcriptomic analysis (RNA-seq) Ttr Msh3 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Lyubov N. Chuvakova Sergei Yu Funikov Alexander P. Rezvykh Alexander P. Rezvykh Artem I. Davletshin Michael B. Evgen’ev Svetlana A. Litvinova Irina B. Fedotova Inga I. Poletaeva David G. Garbuz Transcriptome of the Krushinsky-Molodkina Audiogenic Rat Strain and Identification of Possible Audiogenic Epilepsy-Associated Genes
description	Audiogenic epilepsy (AE), inherent to several rodent strains is widely studied as a model of generalized convulsive epilepsy. The molecular mechanisms that determine the manifestation of AE are not well understood. In the present work, we compared transcriptomes from the corpora quadrigemina in the midbrain zone, which are crucial for AE development, to identify genes associated with the AE phenotype. Three rat strains without sound exposure were compared: Krushinsky-Molodkina (KM) strain (100% AE-prone); Wistar outbred rat strain (non-AE prone) and “0” strain (partially AE-prone), selected from F2 KM × Wistar hybrids for their lack of AE. The findings showed that the KM strain gene expression profile exhibited a number of characteristics that differed from those of the Wistar and “0” strain profiles. In particular, the KM rats showed increased expression of a number of genes involved in the positive regulation of the MAPK signaling cascade and genes involved in the positive regulation of apoptotic processes. Another characteristic of the KM strain which differed from that of the Wistar and “0” rats was a multi-fold increase in the expression level of the Ttr gene and a significant decrease in the expression of the Msh3 gene. Decreased expression of a number of oxidative phosphorylation-related genes and a few other genes was also identified in the KM strain. Our data confirm the complex multigenic nature of AE inheritance in rodents. A comparison with data obtained from other independently selected AE-prone rodent strains suggests some common causes for the formation of the audiogenic phenotype.
format	article
author	Lyubov N. Chuvakova Sergei Yu Funikov Alexander P. Rezvykh Alexander P. Rezvykh Artem I. Davletshin Michael B. Evgen’ev Svetlana A. Litvinova Irina B. Fedotova Inga I. Poletaeva David G. Garbuz
author_facet	Lyubov N. Chuvakova Sergei Yu Funikov Alexander P. Rezvykh Alexander P. Rezvykh Artem I. Davletshin Michael B. Evgen’ev Svetlana A. Litvinova Irina B. Fedotova Inga I. Poletaeva David G. Garbuz
author_sort	Lyubov N. Chuvakova
title	Transcriptome of the Krushinsky-Molodkina Audiogenic Rat Strain and Identification of Possible Audiogenic Epilepsy-Associated Genes
title_short	Transcriptome of the Krushinsky-Molodkina Audiogenic Rat Strain and Identification of Possible Audiogenic Epilepsy-Associated Genes
title_full	Transcriptome of the Krushinsky-Molodkina Audiogenic Rat Strain and Identification of Possible Audiogenic Epilepsy-Associated Genes
title_fullStr	Transcriptome of the Krushinsky-Molodkina Audiogenic Rat Strain and Identification of Possible Audiogenic Epilepsy-Associated Genes
title_full_unstemmed	Transcriptome of the Krushinsky-Molodkina Audiogenic Rat Strain and Identification of Possible Audiogenic Epilepsy-Associated Genes
title_sort	transcriptome of the krushinsky-molodkina audiogenic rat strain and identification of possible audiogenic epilepsy-associated genes
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3d398a569e2443a78575af68c3f6c41c
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Transcriptome of the Krushinsky-Molodkina Audiogenic Rat Strain and Identification of Possible Audiogenic Epilepsy-Associated Genes

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