Expression of purinergic receptors on microglia in the animal model of choroidal neovascularisation

Abstract To investigate the effect of P2 receptor on microglia and its inhibitor PPADS on choroidal neovascularization. Forty CX3CR1GFP/+ mice were randomly divided into 8 groups. In addition to the normal group, the rest of groups were receiving laser treatment. The retina and choroid from the seco...

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Autores principales:	Lu Li, Juejun Liu, Amin Xu, Peter Heiduschka, Nicole Eter, Changzheng Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3d43db18894c41248f5ea4a7b0a86d03
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spelling	oai:doaj.org-article:3d43db18894c41248f5ea4a7b0a86d032021-12-02T17:47:23ZExpression of purinergic receptors on microglia in the animal model of choroidal neovascularisation10.1038/s41598-021-91989-42045-2322https://doaj.org/article/3d43db18894c41248f5ea4a7b0a86d032021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91989-4https://doaj.org/toc/2045-2322Abstract To investigate the effect of P2 receptor on microglia and its inhibitor PPADS on choroidal neovascularization. Forty CX3CR1GFP/+ mice were randomly divided into 8 groups. In addition to the normal group, the rest of groups were receiving laser treatment. The retina and choroid from the second, third, fourth and fifth group of mice were taken in the 1, 4, 7, 14 days after laser treatment. The mice in the sixth and seventh group received intravitreal injection of 2 µl PPADS or PBS respectively immediately after laser treatment. The mice in the eighth group received topical application of PPADS once per day of three days. The mice in sixth, seventh and eighth group received AF and FFA examination on the fourth day after laser treatment. Immunofluorescence histochemical staining and real-time quantitative PCR were used to evaluate P2 expression and its effect on choroidal neovascularization. After laser treatment, activated microglia can express P2 receptors (P2X4, P2X7, P2Y2 and P2Y12). The expression of P2 increased on the first day after laser damage, peaked on the fourth day (tP2X4 = 6.05, tP2X7 = 2.95, tP2Y2 = 3.67, tP2Y12 = 5.98, all P < 0.01), and then decreased. After PPADS inhibition, compared with the PBS injection group, the mRNA of P2X4, P2X7, P2Y2 and P2Y12 were decreased significantly in the PPADS injection group (tP2X4 = 5.54, tP2X7 = 9.82, tP2Y2 = 3.86, tP2Y12 = 7.91, all P < 0.01) and the PPADS topical application group (tP2X4 = 3.24, tP2X7 = 5.89, tP2Y2 = 6.75, tP2Y12 = 4.97, all P < 0.01). Compared with the PBS injection group, not only the activity of microglia cells but also the leakage of CNV decreased significantly (P < 0.01) in the PPADS injection group and the PPADS topical application group. But between two PPADS groups, the leakage of CNV had no difference (P = 0.864). After laser induced CNV, activated microglia can express P2 receptors. The P2 receptor inhibitor, PPADS, can significantly affect the function of microglia and inhibit the formation of choroidal neovascularization.Lu LiJuejun LiuAmin XuPeter HeiduschkaNicole EterChangzheng ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lu Li Juejun Liu Amin Xu Peter Heiduschka Nicole Eter Changzheng Chen Expression of purinergic receptors on microglia in the animal model of choroidal neovascularisation
description	Abstract To investigate the effect of P2 receptor on microglia and its inhibitor PPADS on choroidal neovascularization. Forty CX3CR1GFP/+ mice were randomly divided into 8 groups. In addition to the normal group, the rest of groups were receiving laser treatment. The retina and choroid from the second, third, fourth and fifth group of mice were taken in the 1, 4, 7, 14 days after laser treatment. The mice in the sixth and seventh group received intravitreal injection of 2 µl PPADS or PBS respectively immediately after laser treatment. The mice in the eighth group received topical application of PPADS once per day of three days. The mice in sixth, seventh and eighth group received AF and FFA examination on the fourth day after laser treatment. Immunofluorescence histochemical staining and real-time quantitative PCR were used to evaluate P2 expression and its effect on choroidal neovascularization. After laser treatment, activated microglia can express P2 receptors (P2X4, P2X7, P2Y2 and P2Y12). The expression of P2 increased on the first day after laser damage, peaked on the fourth day (tP2X4 = 6.05, tP2X7 = 2.95, tP2Y2 = 3.67, tP2Y12 = 5.98, all P < 0.01), and then decreased. After PPADS inhibition, compared with the PBS injection group, the mRNA of P2X4, P2X7, P2Y2 and P2Y12 were decreased significantly in the PPADS injection group (tP2X4 = 5.54, tP2X7 = 9.82, tP2Y2 = 3.86, tP2Y12 = 7.91, all P < 0.01) and the PPADS topical application group (tP2X4 = 3.24, tP2X7 = 5.89, tP2Y2 = 6.75, tP2Y12 = 4.97, all P < 0.01). Compared with the PBS injection group, not only the activity of microglia cells but also the leakage of CNV decreased significantly (P < 0.01) in the PPADS injection group and the PPADS topical application group. But between two PPADS groups, the leakage of CNV had no difference (P = 0.864). After laser induced CNV, activated microglia can express P2 receptors. The P2 receptor inhibitor, PPADS, can significantly affect the function of microglia and inhibit the formation of choroidal neovascularization.
format	article
author	Lu Li Juejun Liu Amin Xu Peter Heiduschka Nicole Eter Changzheng Chen
author_facet	Lu Li Juejun Liu Amin Xu Peter Heiduschka Nicole Eter Changzheng Chen
author_sort	Lu Li
title	Expression of purinergic receptors on microglia in the animal model of choroidal neovascularisation
title_short	Expression of purinergic receptors on microglia in the animal model of choroidal neovascularisation
title_full	Expression of purinergic receptors on microglia in the animal model of choroidal neovascularisation
title_fullStr	Expression of purinergic receptors on microglia in the animal model of choroidal neovascularisation
title_full_unstemmed	Expression of purinergic receptors on microglia in the animal model of choroidal neovascularisation
title_sort	expression of purinergic receptors on microglia in the animal model of choroidal neovascularisation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3d43db18894c41248f5ea4a7b0a86d03
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_version_	1718379459456794624

Expression of purinergic receptors on microglia in the animal model of choroidal neovascularisation

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