High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams

Kato, Miyazaki, Hamaguchi et al. report the structure of Photosystem II in solution at 1.95 Å resolution by single-particle cryo-electron microscopy. They find that reducing the electron beam dosage decreases the electron beam damage while keeping the resolution of the cryo-EM structure, providing i...

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Autores principales:	Koji Kato, Naoyuki Miyazaki, Tasuku Hamaguchi, Yoshiki Nakajima, Fusamichi Akita, Koji Yonekura, Jian-Ren Shen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3d455b2a1f944fa7b8cadda140da7248
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spelling	oai:doaj.org-article:3d455b2a1f944fa7b8cadda140da72482021-12-02T16:36:02ZHigh-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams10.1038/s42003-021-01919-32399-3642https://doaj.org/article/3d455b2a1f944fa7b8cadda140da72482021-03-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01919-3https://doaj.org/toc/2399-3642Kato, Miyazaki, Hamaguchi et al. report the structure of Photosystem II in solution at 1.95 Å resolution by single-particle cryo-electron microscopy. They find that reducing the electron beam dosage decreases the electron beam damage while keeping the resolution of the cryo-EM structure, providing insights into the best practice for the determination of cryo-EM structures.Koji KatoNaoyuki MiyazakiTasuku HamaguchiYoshiki NakajimaFusamichi AkitaKoji YonekuraJian-Ren ShenNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Koji Kato Naoyuki Miyazaki Tasuku Hamaguchi Yoshiki Nakajima Fusamichi Akita Koji Yonekura Jian-Ren Shen High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams
description	Kato, Miyazaki, Hamaguchi et al. report the structure of Photosystem II in solution at 1.95 Å resolution by single-particle cryo-electron microscopy. They find that reducing the electron beam dosage decreases the electron beam damage while keeping the resolution of the cryo-EM structure, providing insights into the best practice for the determination of cryo-EM structures.
format	article
author	Koji Kato Naoyuki Miyazaki Tasuku Hamaguchi Yoshiki Nakajima Fusamichi Akita Koji Yonekura Jian-Ren Shen
author_facet	Koji Kato Naoyuki Miyazaki Tasuku Hamaguchi Yoshiki Nakajima Fusamichi Akita Koji Yonekura Jian-Ren Shen
author_sort	Koji Kato
title	High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams
title_short	High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams
title_full	High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams
title_fullStr	High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams
title_full_unstemmed	High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams
title_sort	high-resolution cryo-em structure of photosystem ii reveals damage from high-dose electron beams
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3d455b2a1f944fa7b8cadda140da7248
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High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams

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