Interplay between toxin transport and flotillin localization.

The flotillin proteins are localized in lipid domains at the plasma membrane as well as in intracellular compartments. In the present study, we examined the importance of flotillin-1 and flotillin-2 for the uptake and transport of the bacterial Shiga toxin (Stx) and the plant toxin ricin and we inve...

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Auteurs principaux:	Sascha Pust, Anne Berit Dyve, Maria L Torgersen, Bo van Deurs, Kirsten Sandvig
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2010
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/3d511d659c5445b1a82700d2d10facd7
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spelling	oai:doaj.org-article:3d511d659c5445b1a82700d2d10facd72021-11-25T06:26:28ZInterplay between toxin transport and flotillin localization.1932-620310.1371/journal.pone.0008844https://doaj.org/article/3d511d659c5445b1a82700d2d10facd72010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20107503/?tool=EBIhttps://doaj.org/toc/1932-6203The flotillin proteins are localized in lipid domains at the plasma membrane as well as in intracellular compartments. In the present study, we examined the importance of flotillin-1 and flotillin-2 for the uptake and transport of the bacterial Shiga toxin (Stx) and the plant toxin ricin and we investigated whether toxin binding and uptake were associated with flotillin relocalization. We observed a toxin-induced redistribution of the flotillins, which seemed to be regulated in a p38-dependent manner. Our experiments provide no evidence for a changed endocytic uptake of Stx or ricin in cells silenced for flotillin-1 or -2. However, the Golgi-dependent sulfation of both toxins was significantly reduced in flotillin knockdown cells. Interestingly, when the transport of ricin to the ER was investigated, we obtained an increased mannosylation of ricin in flotillin-1 and flotillin-2 knockdown cells. The toxicity of both toxins was twofold increased in flotillin-depleted cells. Since BFA (Brefeldin A) inhibits the toxicity even in flotillin knockdown cells, the retrograde toxin transport is apparently still Golgi-dependent. Thus, flotillin proteins regulate and facilitate the retrograde transport of Stx and ricin.Sascha PustAnne Berit DyveMaria L TorgersenBo van DeursKirsten SandvigPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 1, p e8844 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sascha Pust Anne Berit Dyve Maria L Torgersen Bo van Deurs Kirsten Sandvig Interplay between toxin transport and flotillin localization.
description	The flotillin proteins are localized in lipid domains at the plasma membrane as well as in intracellular compartments. In the present study, we examined the importance of flotillin-1 and flotillin-2 for the uptake and transport of the bacterial Shiga toxin (Stx) and the plant toxin ricin and we investigated whether toxin binding and uptake were associated with flotillin relocalization. We observed a toxin-induced redistribution of the flotillins, which seemed to be regulated in a p38-dependent manner. Our experiments provide no evidence for a changed endocytic uptake of Stx or ricin in cells silenced for flotillin-1 or -2. However, the Golgi-dependent sulfation of both toxins was significantly reduced in flotillin knockdown cells. Interestingly, when the transport of ricin to the ER was investigated, we obtained an increased mannosylation of ricin in flotillin-1 and flotillin-2 knockdown cells. The toxicity of both toxins was twofold increased in flotillin-depleted cells. Since BFA (Brefeldin A) inhibits the toxicity even in flotillin knockdown cells, the retrograde toxin transport is apparently still Golgi-dependent. Thus, flotillin proteins regulate and facilitate the retrograde transport of Stx and ricin.
format	article
author	Sascha Pust Anne Berit Dyve Maria L Torgersen Bo van Deurs Kirsten Sandvig
author_facet	Sascha Pust Anne Berit Dyve Maria L Torgersen Bo van Deurs Kirsten Sandvig
author_sort	Sascha Pust
title	Interplay between toxin transport and flotillin localization.
title_short	Interplay between toxin transport and flotillin localization.
title_full	Interplay between toxin transport and flotillin localization.
title_fullStr	Interplay between toxin transport and flotillin localization.
title_full_unstemmed	Interplay between toxin transport and flotillin localization.
title_sort	interplay between toxin transport and flotillin localization.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/3d511d659c5445b1a82700d2d10facd7
work_keys_str_mv	AT saschapust interplaybetweentoxintransportandflotillinlocalization AT anneberitdyve interplaybetweentoxintransportandflotillinlocalization AT marialtorgersen interplaybetweentoxintransportandflotillinlocalization AT bovandeurs interplaybetweentoxintransportandflotillinlocalization AT kirstensandvig interplaybetweentoxintransportandflotillinlocalization
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Interplay between toxin transport and flotillin localization.

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