Pressure Adaptations in Deep-Sea <i>Moritella</i> Dihydrofolate Reductases: Compressibility versus Stability

Proteins from “pressure-loving” piezophiles appear to adapt by greater compressibility via larger total cavity volume. However, larger cavities in proteins have been associated with lower unfolding pressures. Here, dihydrofolate reductase (DHFR) from a moderate piezophile <i>Moritella profunda...

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Autores principales: Ryan W. Penhallurick, Toshiko Ichiye
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Publicado: MDPI AG 2021
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spelling oai:doaj.org-article:3d535b27787c4d6f9c5a1774258493072021-11-25T16:48:09ZPressure Adaptations in Deep-Sea <i>Moritella</i> Dihydrofolate Reductases: Compressibility versus Stability10.3390/biology101112112079-7737https://doaj.org/article/3d535b27787c4d6f9c5a1774258493072021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1211https://doaj.org/toc/2079-7737Proteins from “pressure-loving” piezophiles appear to adapt by greater compressibility via larger total cavity volume. However, larger cavities in proteins have been associated with lower unfolding pressures. Here, dihydrofolate reductase (DHFR) from a moderate piezophile <i>Moritella profunda</i> (Mp) isolated at ~2.9 km in depth and from a hyperpiezophile <i>Moritella yayanosii</i> (My) isolated at ~11 km in depth were compared using molecular dynamics simulations. Although previous simulations indicate that MpDHFR is more compressible than a mesophile DHFR, here the average properties and a quasiharmonic analysis indicate that MpDHFR and MyDHFR have similar compressibilities. A cavity analysis also indicates that the three unique mutations in MyDHFR are near cavities, although the cavities are generally similar in size in both. However, while a cleft overlaps an internal cavity, thus forming a pathway from the surface to the interior in MpDHFR, the unique residue Tyr103 found in MyDHFR forms a hydrogen bond with Leu78, and the sidechain separates the cleft from the cavity. Thus, while <i>Moritella</i> DHFR may generally be well suited to high-pressure environments because of their greater compressibility, adaptation for greater depths may be to prevent water entry into the interior cavities.Ryan W. PenhallurickToshiko IchiyeMDPI AGarticledeep-sea adaptationscompressibilitycavitiespressurepotential energy landscapeBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1211, p 1211 (2021)
institution DOAJ
collection DOAJ
language EN
topic deep-sea adaptations
compressibility
cavities
pressure
potential energy landscape
Biology (General)
QH301-705.5
spellingShingle deep-sea adaptations
compressibility
cavities
pressure
potential energy landscape
Biology (General)
QH301-705.5
Ryan W. Penhallurick
Toshiko Ichiye
Pressure Adaptations in Deep-Sea <i>Moritella</i> Dihydrofolate Reductases: Compressibility versus Stability
description Proteins from “pressure-loving” piezophiles appear to adapt by greater compressibility via larger total cavity volume. However, larger cavities in proteins have been associated with lower unfolding pressures. Here, dihydrofolate reductase (DHFR) from a moderate piezophile <i>Moritella profunda</i> (Mp) isolated at ~2.9 km in depth and from a hyperpiezophile <i>Moritella yayanosii</i> (My) isolated at ~11 km in depth were compared using molecular dynamics simulations. Although previous simulations indicate that MpDHFR is more compressible than a mesophile DHFR, here the average properties and a quasiharmonic analysis indicate that MpDHFR and MyDHFR have similar compressibilities. A cavity analysis also indicates that the three unique mutations in MyDHFR are near cavities, although the cavities are generally similar in size in both. However, while a cleft overlaps an internal cavity, thus forming a pathway from the surface to the interior in MpDHFR, the unique residue Tyr103 found in MyDHFR forms a hydrogen bond with Leu78, and the sidechain separates the cleft from the cavity. Thus, while <i>Moritella</i> DHFR may generally be well suited to high-pressure environments because of their greater compressibility, adaptation for greater depths may be to prevent water entry into the interior cavities.
format article
author Ryan W. Penhallurick
Toshiko Ichiye
author_facet Ryan W. Penhallurick
Toshiko Ichiye
author_sort Ryan W. Penhallurick
title Pressure Adaptations in Deep-Sea <i>Moritella</i> Dihydrofolate Reductases: Compressibility versus Stability
title_short Pressure Adaptations in Deep-Sea <i>Moritella</i> Dihydrofolate Reductases: Compressibility versus Stability
title_full Pressure Adaptations in Deep-Sea <i>Moritella</i> Dihydrofolate Reductases: Compressibility versus Stability
title_fullStr Pressure Adaptations in Deep-Sea <i>Moritella</i> Dihydrofolate Reductases: Compressibility versus Stability
title_full_unstemmed Pressure Adaptations in Deep-Sea <i>Moritella</i> Dihydrofolate Reductases: Compressibility versus Stability
title_sort pressure adaptations in deep-sea <i>moritella</i> dihydrofolate reductases: compressibility versus stability
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/3d535b27787c4d6f9c5a177425849307
work_keys_str_mv AT ryanwpenhallurick pressureadaptationsindeepseaimoritellaidihydrofolatereductasescompressibilityversusstability
AT toshikoichiye pressureadaptationsindeepseaimoritellaidihydrofolatereductasescompressibilityversusstability
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