Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea

Fulwah Alqahtani,1,* Fadilah Aleanizy,1,* Eram El Tahir,1 Hiba Alhabib,1 Raghad Alsaif,1 Gamal Shazly,1,2 Hajar AlQahtani,3 Ibrahim Alsarra,1 Jafar Mahdavi4 1Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; 2Department of Industrial Pharmacy, Faculty of P...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alqahtani F, Aleanizy F, El Tahir E, Alhabib H, Alsaif R, Shazly G, AlQahtani H, Alsarra I, Mahdavi J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	n. gonorrhoeae chitosan nanoparticles antimicrobial adhesion Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3d5a0014b3b84c0292374742027e144d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3d5a0014b3b84c0292374742027e144d
record_format	dspace
spelling	oai:doaj.org-article:3d5a0014b3b84c0292374742027e144d2021-12-02T12:42:03ZAntibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea1178-2013https://doaj.org/article/3d5a0014b3b84c0292374742027e144d2020-10-01T00:00:00Zhttps://www.dovepress.com/antibacterial-activity-of-chitosan-nanoparticles-against-pathogenic-n--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fulwah Alqahtani,1,* Fadilah Aleanizy,1,* Eram El Tahir,1 Hiba Alhabib,1 Raghad Alsaif,1 Gamal Shazly,1,2 Hajar AlQahtani,3 Ibrahim Alsarra,1 Jafar Mahdavi4 1Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; 2Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt; 3Department of Pharmacy Services, King Abdul-Aziz Medical City, Ministry of National Guard, Health Affairs, Riyadh, Saudi Arabia; 4School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, UK*These authors contributed equally to this workCorrespondence: Fulwah AlqahtaniDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box: 22452, Riyadh 11495, Saudi ArabiaTel +966-11-8051478Email fyalqahtani@ksu.edu.saPurpose: The emergence of Neisseria gonorrhoeae strains that are resistant to the most commonly used antibiotics represents a great concern for global public health. This challenges the effectiveness of clinical treatment regimens and demands the development of alternative antigonococcal agent. In this regard, chitosan nanoparticles (CNPs) are known to have antimicrobial activity against a wide range of pathogens. Thus, they have become a potential candidate for combatting this era of multi-drug resistance. This study aims to formulate CNPs, characterize their physicochemical properties, and examine their antimicrobial activity against gonococcus.Materials and Methods: The ionic gelation method was used to prepare CNPs of different concentrations. Characterization for their particle size (PZ), polydispersity index (PDI), and zeta potential (ZP) was performed. The anti-microbial activity of CNPs was investigated against 13 WHO N. gonorrhoeae reference strains, using the broth dilution method. Cytotoxicity of CNPs and their effect on bacterial adhesion to HeLa cells were investigated.Results: The average PZ and ZP of the prepared NPs were increased when the concentration of chitosan was increased from 1 to 5 mg/mL and found to be in the range of 193 nm ± 1.9 to 530 nm ± 13.3, and 14 mV ± 0.5 to 20 mV ± 1, respectively. Transmission electron microscopes (TEM) images revealed spherical NPs, and the NPs had a low PDI value of ≤ 0.27. The formed CNPs produced antibacterial activity against all tested strains, including those resistant to multiple antibiotics, with a minimum inhibitory concentration (MIC90) of 0.16 to 0.31 mg/mL and a minimum bactericidal concentration (MBC) of 0.31 to 0.61 mg/mL. Of note, at all MIC90 and MBC, the CNPs had no significant cytotoxic effect on HeLa cells and reduced bacterial adhesion to these cells at MBC doses.Conclusion: The present work findings suggest the potential of the CNPs for the treatment of gonorrhoea.Keywords: N. gonorrhoeae, chitosan nanoparticles, antimicrobial, adhesionAlqahtani FAleanizy FEl Tahir EAlhabib HAlsaif RShazly GAlQahtani HAlsarra IMahdavi JDove Medical Pressarticlen. gonorrhoeaechitosan nanoparticlesantimicrobialadhesionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 7877-7887 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	n. gonorrhoeae chitosan nanoparticles antimicrobial adhesion Medicine (General) R5-920
spellingShingle	n. gonorrhoeae chitosan nanoparticles antimicrobial adhesion Medicine (General) R5-920 Alqahtani F Aleanizy F El Tahir E Alhabib H Alsaif R Shazly G AlQahtani H Alsarra I Mahdavi J Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
description	Fulwah Alqahtani,1,* Fadilah Aleanizy,1,* Eram El Tahir,1 Hiba Alhabib,1 Raghad Alsaif,1 Gamal Shazly,1,2 Hajar AlQahtani,3 Ibrahim Alsarra,1 Jafar Mahdavi4 1Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; 2Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt; 3Department of Pharmacy Services, King Abdul-Aziz Medical City, Ministry of National Guard, Health Affairs, Riyadh, Saudi Arabia; 4School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, UK*These authors contributed equally to this workCorrespondence: Fulwah AlqahtaniDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box: 22452, Riyadh 11495, Saudi ArabiaTel +966-11-8051478Email fyalqahtani@ksu.edu.saPurpose: The emergence of Neisseria gonorrhoeae strains that are resistant to the most commonly used antibiotics represents a great concern for global public health. This challenges the effectiveness of clinical treatment regimens and demands the development of alternative antigonococcal agent. In this regard, chitosan nanoparticles (CNPs) are known to have antimicrobial activity against a wide range of pathogens. Thus, they have become a potential candidate for combatting this era of multi-drug resistance. This study aims to formulate CNPs, characterize their physicochemical properties, and examine their antimicrobial activity against gonococcus.Materials and Methods: The ionic gelation method was used to prepare CNPs of different concentrations. Characterization for their particle size (PZ), polydispersity index (PDI), and zeta potential (ZP) was performed. The anti-microbial activity of CNPs was investigated against 13 WHO N. gonorrhoeae reference strains, using the broth dilution method. Cytotoxicity of CNPs and their effect on bacterial adhesion to HeLa cells were investigated.Results: The average PZ and ZP of the prepared NPs were increased when the concentration of chitosan was increased from 1 to 5 mg/mL and found to be in the range of 193 nm ± 1.9 to 530 nm ± 13.3, and 14 mV ± 0.5 to 20 mV ± 1, respectively. Transmission electron microscopes (TEM) images revealed spherical NPs, and the NPs had a low PDI value of ≤ 0.27. The formed CNPs produced antibacterial activity against all tested strains, including those resistant to multiple antibiotics, with a minimum inhibitory concentration (MIC90) of 0.16 to 0.31 mg/mL and a minimum bactericidal concentration (MBC) of 0.31 to 0.61 mg/mL. Of note, at all MIC90 and MBC, the CNPs had no significant cytotoxic effect on HeLa cells and reduced bacterial adhesion to these cells at MBC doses.Conclusion: The present work findings suggest the potential of the CNPs for the treatment of gonorrhoea.Keywords: N. gonorrhoeae, chitosan nanoparticles, antimicrobial, adhesion
format	article
author	Alqahtani F Aleanizy F El Tahir E Alhabib H Alsaif R Shazly G AlQahtani H Alsarra I Mahdavi J
author_facet	Alqahtani F Aleanizy F El Tahir E Alhabib H Alsaif R Shazly G AlQahtani H Alsarra I Mahdavi J
author_sort	Alqahtani F
title	Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
title_short	Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
title_full	Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
title_fullStr	Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
title_full_unstemmed	Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea
title_sort	antibacterial activity of chitosan nanoparticles against pathogenic n. gonorrhoea
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/3d5a0014b3b84c0292374742027e144d
work_keys_str_mv	AT alqahtanif antibacterialactivityofchitosannanoparticlesagainstpathogenicngonorrhoea AT aleanizyf antibacterialactivityofchitosannanoparticlesagainstpathogenicngonorrhoea AT eltahire antibacterialactivityofchitosannanoparticlesagainstpathogenicngonorrhoea AT alhabibh antibacterialactivityofchitosannanoparticlesagainstpathogenicngonorrhoea AT alsaifr antibacterialactivityofchitosannanoparticlesagainstpathogenicngonorrhoea AT shazlyg antibacterialactivityofchitosannanoparticlesagainstpathogenicngonorrhoea AT alqahtanih antibacterialactivityofchitosannanoparticlesagainstpathogenicngonorrhoea AT alsarrai antibacterialactivityofchitosannanoparticlesagainstpathogenicngonorrhoea AT mahdavij antibacterialactivityofchitosannanoparticlesagainstpathogenicngonorrhoea
_version_	1718393679516794880

Antibacterial Activity of Chitosan Nanoparticles Against Pathogenic N. gonorrhoea

Ejemplares similares