Circulating microRNAs in Symptomatic and Asymptomatic Carotid Stenosis

Background: Specific microRNAs (miRs) have been implicated in the pathophysiology of atherosclerosis and may represent interesting diagnostic and therapeutic targets in carotid stenosis. We hypothesized that the levels of specific circulating miRs are altered in patients with symptomatic carotid ste...

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Autores principales:	Gerrit M. Grosse, Anselm A. Derda, Ricarda D. Stauss, Lavinia Neubert, Danny D. Jonigk, Mark P. Kühnel, Maria M. Gabriel, Ramona Schuppner, Mathias Wilhelmi, Christian Bär, Johann Bauersachs, Claudia Schrimpf, Thomas Thum, Karin Weissenborn
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	atherosclerosis biomarker carotid stenosis microRNA stroke Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/3d5e3aa769e04f7bb7d2aa1544eaca79
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spelling	oai:doaj.org-article:3d5e3aa769e04f7bb7d2aa1544eaca792021-11-30T17:02:10ZCirculating microRNAs in Symptomatic and Asymptomatic Carotid Stenosis1664-229510.3389/fneur.2021.755827https://doaj.org/article/3d5e3aa769e04f7bb7d2aa1544eaca792021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fneur.2021.755827/fullhttps://doaj.org/toc/1664-2295Background: Specific microRNAs (miRs) have been implicated in the pathophysiology of atherosclerosis and may represent interesting diagnostic and therapeutic targets in carotid stenosis. We hypothesized that the levels of specific circulating miRs are altered in patients with symptomatic carotid stenosis (sCS) in comparison to those in patients with asymptomatic carotid stenosis (aCS) planned to undergo carotid endarterectomy (CEA). We also studied whether miR levels are associated with plaque vulnerability and stability over time after CEA.Methods: Circulating levels of vascular-enriched miR-92a, miR-126, miR-143, miR-145, miR-155, miR-210, miR-221, miR-222, and miR-342-3p were determined in 21 patients with sCS and 23 patients with aCS before CEA and at a 90-day follow-up. Transcranial Doppler ultrasound for detection of microembolic signals (MES) in the ipsilateral middle cerebral artery was performed prior to CEA. Carotid plaques were histologically analyzed.Results: Mean levels of miRs were not considerably different between groups and were only marginally higher in sCS than aCS concerning miR-92a, miR-210, miR-145, and miR-143 with the best evidence concerning miR-92a. After adjustment for vascular risk factors and statin pre-treatment, the effect sizes remained essentially unchanged. At follow-up, however, these modest differences remained uncorroborated. There were no relevant associations between miR-levels and MES or histological plaque vulnerability features.Conclusions: This study does not provide evidence for strong associations between specific circulating miRs and symptomatic state in a collective of comprehensively characterized patients with carotid stenosis. Further work is needed to elucidate the role of circulating miRs as targets in advanced carotid atherosclerosis.Gerrit M. GrosseAnselm A. DerdaAnselm A. DerdaRicarda D. StaussLavinia NeubertDanny D. JonigkMark P. KühnelMaria M. GabrielRamona SchuppnerMathias WilhelmiMathias WilhelmiChristian BärChristian BärJohann BauersachsClaudia SchrimpfClaudia SchrimpfThomas ThumThomas ThumThomas ThumKarin WeissenbornFrontiers Media S.A.articleatherosclerosisbiomarkercarotid stenosismicroRNAstrokeNeurology. Diseases of the nervous systemRC346-429ENFrontiers in Neurology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	atherosclerosis biomarker carotid stenosis microRNA stroke Neurology. Diseases of the nervous system RC346-429
spellingShingle	atherosclerosis biomarker carotid stenosis microRNA stroke Neurology. Diseases of the nervous system RC346-429 Gerrit M. Grosse Anselm A. Derda Anselm A. Derda Ricarda D. Stauss Lavinia Neubert Danny D. Jonigk Mark P. Kühnel Maria M. Gabriel Ramona Schuppner Mathias Wilhelmi Mathias Wilhelmi Christian Bär Christian Bär Johann Bauersachs Claudia Schrimpf Claudia Schrimpf Thomas Thum Thomas Thum Thomas Thum Karin Weissenborn Circulating microRNAs in Symptomatic and Asymptomatic Carotid Stenosis
description	Background: Specific microRNAs (miRs) have been implicated in the pathophysiology of atherosclerosis and may represent interesting diagnostic and therapeutic targets in carotid stenosis. We hypothesized that the levels of specific circulating miRs are altered in patients with symptomatic carotid stenosis (sCS) in comparison to those in patients with asymptomatic carotid stenosis (aCS) planned to undergo carotid endarterectomy (CEA). We also studied whether miR levels are associated with plaque vulnerability and stability over time after CEA.Methods: Circulating levels of vascular-enriched miR-92a, miR-126, miR-143, miR-145, miR-155, miR-210, miR-221, miR-222, and miR-342-3p were determined in 21 patients with sCS and 23 patients with aCS before CEA and at a 90-day follow-up. Transcranial Doppler ultrasound for detection of microembolic signals (MES) in the ipsilateral middle cerebral artery was performed prior to CEA. Carotid plaques were histologically analyzed.Results: Mean levels of miRs were not considerably different between groups and were only marginally higher in sCS than aCS concerning miR-92a, miR-210, miR-145, and miR-143 with the best evidence concerning miR-92a. After adjustment for vascular risk factors and statin pre-treatment, the effect sizes remained essentially unchanged. At follow-up, however, these modest differences remained uncorroborated. There were no relevant associations between miR-levels and MES or histological plaque vulnerability features.Conclusions: This study does not provide evidence for strong associations between specific circulating miRs and symptomatic state in a collective of comprehensively characterized patients with carotid stenosis. Further work is needed to elucidate the role of circulating miRs as targets in advanced carotid atherosclerosis.
format	article
author	Gerrit M. Grosse Anselm A. Derda Anselm A. Derda Ricarda D. Stauss Lavinia Neubert Danny D. Jonigk Mark P. Kühnel Maria M. Gabriel Ramona Schuppner Mathias Wilhelmi Mathias Wilhelmi Christian Bär Christian Bär Johann Bauersachs Claudia Schrimpf Claudia Schrimpf Thomas Thum Thomas Thum Thomas Thum Karin Weissenborn
author_facet	Gerrit M. Grosse Anselm A. Derda Anselm A. Derda Ricarda D. Stauss Lavinia Neubert Danny D. Jonigk Mark P. Kühnel Maria M. Gabriel Ramona Schuppner Mathias Wilhelmi Mathias Wilhelmi Christian Bär Christian Bär Johann Bauersachs Claudia Schrimpf Claudia Schrimpf Thomas Thum Thomas Thum Thomas Thum Karin Weissenborn
author_sort	Gerrit M. Grosse
title	Circulating microRNAs in Symptomatic and Asymptomatic Carotid Stenosis
title_short	Circulating microRNAs in Symptomatic and Asymptomatic Carotid Stenosis
title_full	Circulating microRNAs in Symptomatic and Asymptomatic Carotid Stenosis
title_fullStr	Circulating microRNAs in Symptomatic and Asymptomatic Carotid Stenosis
title_full_unstemmed	Circulating microRNAs in Symptomatic and Asymptomatic Carotid Stenosis
title_sort	circulating micrornas in symptomatic and asymptomatic carotid stenosis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3d5e3aa769e04f7bb7d2aa1544eaca79
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Circulating microRNAs in Symptomatic and Asymptomatic Carotid Stenosis

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