A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR

Abstract The technique RT-qPCR for viral RNA detection is the current worldwide strategy used for early detection of the novel coronavirus SARS-CoV-2. RNA extraction is a key pre-analytical step in RT-qPCR, often achieved using commercial kits. However, the magnitude of the COVID-19 pandemic is caus...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aniela Wozniak, Ariel Cerda, Catalina Ibarra-Henríquez, Valentina Sebastian, Grace Armijo, Liliana Lamig, Carolina Miranda, Marcela Lagos, Sandra Solari, Ana María Guzmán, Teresa Quiroga, Susan Hitschfeld, Eleodoro Riveras, Marcela Ferrés, Rodrigo A. Gutiérrez, Patricia García
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3d6ccef257334da299e71a1f0d5a5116
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3d6ccef257334da299e71a1f0d5a5116
record_format	dspace
spelling	oai:doaj.org-article:3d6ccef257334da299e71a1f0d5a51162021-12-02T18:37:06ZA simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR10.1038/s41598-020-73616-w2045-2322https://doaj.org/article/3d6ccef257334da299e71a1f0d5a51162020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73616-whttps://doaj.org/toc/2045-2322Abstract The technique RT-qPCR for viral RNA detection is the current worldwide strategy used for early detection of the novel coronavirus SARS-CoV-2. RNA extraction is a key pre-analytical step in RT-qPCR, often achieved using commercial kits. However, the magnitude of the COVID-19 pandemic is causing disruptions to the global supply chains used by many diagnostic laboratories to procure the commercial kits required for RNA extraction. Shortage in these essential reagents is even more acute in developing countries with no means to produce kits locally. We sought to find an alternative procedure to replace commercial kits using common reagents found in molecular biology laboratories. Here we report a method for RNA extraction that takes about 40 min to complete ten samples, and is not more laborious than current commercial RNA extraction kits. We demonstrate that this method can be used to process nasopharyngeal swab samples and yields RT-qPCR results comparable to those obtained with commercial kits. Most importantly, this procedure can be easily implemented in any molecular diagnostic laboratory. Frequent testing is crucial for individual patient management as well as for public health decision making in this pandemic. Implementation of this method could maintain crucial testing going despite commercial kit shortages.Aniela WozniakAriel CerdaCatalina Ibarra-HenríquezValentina SebastianGrace ArmijoLiliana LamigCarolina MirandaMarcela LagosSandra SolariAna María GuzmánTeresa QuirogaSusan HitschfeldEleodoro RiverasMarcela FerrésRodrigo A. GutiérrezPatricia GarcíaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aniela Wozniak Ariel Cerda Catalina Ibarra-Henríquez Valentina Sebastian Grace Armijo Liliana Lamig Carolina Miranda Marcela Lagos Sandra Solari Ana María Guzmán Teresa Quiroga Susan Hitschfeld Eleodoro Riveras Marcela Ferrés Rodrigo A. Gutiérrez Patricia García A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR
description	Abstract The technique RT-qPCR for viral RNA detection is the current worldwide strategy used for early detection of the novel coronavirus SARS-CoV-2. RNA extraction is a key pre-analytical step in RT-qPCR, often achieved using commercial kits. However, the magnitude of the COVID-19 pandemic is causing disruptions to the global supply chains used by many diagnostic laboratories to procure the commercial kits required for RNA extraction. Shortage in these essential reagents is even more acute in developing countries with no means to produce kits locally. We sought to find an alternative procedure to replace commercial kits using common reagents found in molecular biology laboratories. Here we report a method for RNA extraction that takes about 40 min to complete ten samples, and is not more laborious than current commercial RNA extraction kits. We demonstrate that this method can be used to process nasopharyngeal swab samples and yields RT-qPCR results comparable to those obtained with commercial kits. Most importantly, this procedure can be easily implemented in any molecular diagnostic laboratory. Frequent testing is crucial for individual patient management as well as for public health decision making in this pandemic. Implementation of this method could maintain crucial testing going despite commercial kit shortages.
format	article
author	Aniela Wozniak Ariel Cerda Catalina Ibarra-Henríquez Valentina Sebastian Grace Armijo Liliana Lamig Carolina Miranda Marcela Lagos Sandra Solari Ana María Guzmán Teresa Quiroga Susan Hitschfeld Eleodoro Riveras Marcela Ferrés Rodrigo A. Gutiérrez Patricia García
author_facet	Aniela Wozniak Ariel Cerda Catalina Ibarra-Henríquez Valentina Sebastian Grace Armijo Liliana Lamig Carolina Miranda Marcela Lagos Sandra Solari Ana María Guzmán Teresa Quiroga Susan Hitschfeld Eleodoro Riveras Marcela Ferrés Rodrigo A. Gutiérrez Patricia García
author_sort	Aniela Wozniak
title	A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR
title_short	A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR
title_full	A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR
title_fullStr	A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR
title_full_unstemmed	A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR
title_sort	simple rna preparation method for sars-cov-2 detection by rt-qpcr
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/3d6ccef257334da299e71a1f0d5a5116
work_keys_str_mv	AT anielawozniak asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT arielcerda asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT catalinaibarrahenriquez asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT valentinasebastian asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT gracearmijo asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT lilianalamig asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT carolinamiranda asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT marcelalagos asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT sandrasolari asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT anamariaguzman asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT teresaquiroga asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT susanhitschfeld asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT eleodororiveras asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT marcelaferres asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT rodrigoagutierrez asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT patriciagarcia asimplernapreparationmethodforsarscov2detectionbyrtqpcr AT anielawozniak simplernapreparationmethodforsarscov2detectionbyrtqpcr AT arielcerda simplernapreparationmethodforsarscov2detectionbyrtqpcr AT catalinaibarrahenriquez simplernapreparationmethodforsarscov2detectionbyrtqpcr AT valentinasebastian simplernapreparationmethodforsarscov2detectionbyrtqpcr AT gracearmijo simplernapreparationmethodforsarscov2detectionbyrtqpcr AT lilianalamig simplernapreparationmethodforsarscov2detectionbyrtqpcr AT carolinamiranda simplernapreparationmethodforsarscov2detectionbyrtqpcr AT marcelalagos simplernapreparationmethodforsarscov2detectionbyrtqpcr AT sandrasolari simplernapreparationmethodforsarscov2detectionbyrtqpcr AT anamariaguzman simplernapreparationmethodforsarscov2detectionbyrtqpcr AT teresaquiroga simplernapreparationmethodforsarscov2detectionbyrtqpcr AT susanhitschfeld simplernapreparationmethodforsarscov2detectionbyrtqpcr AT eleodororiveras simplernapreparationmethodforsarscov2detectionbyrtqpcr AT marcelaferres simplernapreparationmethodforsarscov2detectionbyrtqpcr AT rodrigoagutierrez simplernapreparationmethodforsarscov2detectionbyrtqpcr AT patriciagarcia simplernapreparationmethodforsarscov2detectionbyrtqpcr
_version_	1718377792406552576

A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR

Ejemplares similares