Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.

Insulin and related peptides play important and conserved functions in growth and metabolism. Although Drosophila has proved useful for the genetic analysis of insulin functions, little is known about the transcription factors and cell lineages involved in insulin production. Within the embryonic ce...

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Auteurs principaux:	Irene Miguel-Aliaga, Stefan Thor, Alex P Gould
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2008
Sujets:	Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/3d7c53f2520d4ebd98076fa9aca3e694
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spelling	oai:doaj.org-article:3d7c53f2520d4ebd98076fa9aca3e6942021-11-25T05:33:27ZPostmitotic specification of Drosophila insulinergic neurons from pioneer neurons.1544-91731545-788510.1371/journal.pbio.0060058https://doaj.org/article/3d7c53f2520d4ebd98076fa9aca3e6942008-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18336071/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Insulin and related peptides play important and conserved functions in growth and metabolism. Although Drosophila has proved useful for the genetic analysis of insulin functions, little is known about the transcription factors and cell lineages involved in insulin production. Within the embryonic central nervous system, the MP2 neuroblast divides once to generate a dMP2 neuron that initially functions as a pioneer, guiding the axons of other later-born embryonic neurons. Later during development, dMP2 neurons in anterior segments undergo apoptosis but their posterior counterparts persist. We show here that surviving posterior dMP2 neurons no longer function in axonal scaffolding but differentiate into neuroendocrine cells that express insulin-like peptide 7 (Ilp7) and innervate the hindgut. We find that the postmitotic transition from pioneer to insulin-producing neuron is a multistep process requiring retrograde bone morphogenetic protein (BMP) signalling and four transcription factors: Abdominal-B, Hb9, Fork Head, and Dimmed. These five inputs contribute in a partially overlapping manner to combinatorial codes for dMP2 apoptosis, survival, and insulinergic differentiation. Ectopic reconstitution of this code is sufficient to activate Ilp7 expression in other postmitotic neurons. These studies reveal striking similarities between the transcription factors regulating insulin expression in insect neurons and mammalian pancreatic beta-cells.Irene Miguel-AliagaStefan ThorAlex P GouldPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 6, Iss 3, p e58 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Irene Miguel-Aliaga Stefan Thor Alex P Gould Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.
description	Insulin and related peptides play important and conserved functions in growth and metabolism. Although Drosophila has proved useful for the genetic analysis of insulin functions, little is known about the transcription factors and cell lineages involved in insulin production. Within the embryonic central nervous system, the MP2 neuroblast divides once to generate a dMP2 neuron that initially functions as a pioneer, guiding the axons of other later-born embryonic neurons. Later during development, dMP2 neurons in anterior segments undergo apoptosis but their posterior counterparts persist. We show here that surviving posterior dMP2 neurons no longer function in axonal scaffolding but differentiate into neuroendocrine cells that express insulin-like peptide 7 (Ilp7) and innervate the hindgut. We find that the postmitotic transition from pioneer to insulin-producing neuron is a multistep process requiring retrograde bone morphogenetic protein (BMP) signalling and four transcription factors: Abdominal-B, Hb9, Fork Head, and Dimmed. These five inputs contribute in a partially overlapping manner to combinatorial codes for dMP2 apoptosis, survival, and insulinergic differentiation. Ectopic reconstitution of this code is sufficient to activate Ilp7 expression in other postmitotic neurons. These studies reveal striking similarities between the transcription factors regulating insulin expression in insect neurons and mammalian pancreatic beta-cells.
format	article
author	Irene Miguel-Aliaga Stefan Thor Alex P Gould
author_facet	Irene Miguel-Aliaga Stefan Thor Alex P Gould
author_sort	Irene Miguel-Aliaga
title	Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.
title_short	Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.
title_full	Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.
title_fullStr	Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.
title_full_unstemmed	Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.
title_sort	postmitotic specification of drosophila insulinergic neurons from pioneer neurons.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/3d7c53f2520d4ebd98076fa9aca3e694
work_keys_str_mv	AT irenemiguelaliaga postmitoticspecificationofdrosophilainsulinergicneuronsfrompioneerneurons AT stefanthor postmitoticspecificationofdrosophilainsulinergicneuronsfrompioneerneurons AT alexpgould postmitoticspecificationofdrosophilainsulinergicneuronsfrompioneerneurons
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Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons.

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