Magnetic nanoparticle hyperthermia potentiates paclitaxel activity in sensitive and resistant breast cancer cells

Angelie Rivera-Rodriguez,1 Andreina Chiu-Lam,2 Viacheslav M Morozov,3,4 Alexander M Ishov,3,4 Carlos Rinaldi1,2,4 1J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA; 2Department of Chemical Engineering, University of Florida, Gainesville, FL,...

Full description

Saved in:

Bibliographic Details
Main Authors:	Rivera-Rodriguez A, Chiu-Lam A, Morozov VM, Ishov AM, Rinaldi C
Format:	article
Language:	EN
Published:	Dove Medical Press 2018
Subjects:	iron oxide nanoparticles chemotherapy drug resistance hyperthermia Taxanes Medicine (General) R5-920
Online Access:	https://doaj.org/article/3d92dd58f116410c96da167e45e0cde3
Tags:	Add Tag No Tags, Be the first to tag this record!

id	oai:doaj.org-article:3d92dd58f116410c96da167e45e0cde3
record_format	dspace
spelling	oai:doaj.org-article:3d92dd58f116410c96da167e45e0cde32021-12-02T06:51:14ZMagnetic nanoparticle hyperthermia potentiates paclitaxel activity in sensitive and resistant breast cancer cells1178-2013https://doaj.org/article/3d92dd58f116410c96da167e45e0cde32018-08-01T00:00:00Zhttps://www.dovepress.com/magnetic-nanoparticle-hyperthermia-potentiates-paclitaxel-activity-in--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Angelie Rivera-Rodriguez,1 Andreina Chiu-Lam,2 Viacheslav M Morozov,3,4 Alexander M Ishov,3,4 Carlos Rinaldi1,2,4 1J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA; 2Department of Chemical Engineering, University of Florida, Gainesville, FL, USA; 3Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL, USA; 4UF Health Cancer Center Gainesville, FL, USA Introduction: Overcoming resistance to antimitotic drugs, such as paclitaxel (PTX), would represent a major advance in breast cancer treatment. PTX induces mitotic block and sensitive cells exit mitosis dying by mitotic catastrophe. Resistant cells remain in block and continue proliferation after drug decay, denoting one of the PTX resistance mechanisms. Mild hyperthermia (HT) triggers mitotic exit of PTX-pretreated cells, overcoming PTX resistance and suggesting HT-forced mitotic exit as a promising strategy to potentiate PTX. Methods and results: Superparamagnetic iron oxide nanoparticles (SPIONs) were used to deliver mild HT at 42°C in PTX-pretreated breast adenocarcinoma MCF-7 cells sensitive and resistant to PTX. To evaluate mechanism of cell death, cells were classified based on nuclear morphology into interphase, mitotic, micronucleated, and apoptotic. The combined PTX→SPION treatment resulted in an increase in the percentage of micronucleated cells, an indication of forced mitotic exit. Importantly, in PTX-resistant cells, the combination therapy using SPION HT helps to overcome resistance by reducing the number of cells relative to the control. Conclusion: SPION HT potentiates PTX by significantly reducing cell survival, suggesting potential of combined treatment for future clinical translation. Keywords: iron oxide nanoparticles, chemotherapy, drug resistance, hyperthermia, taxanesRivera-Rodriguez AChiu-Lam AMorozov VMIshov AMRinaldi CDove Medical Pressarticleiron oxide nanoparticleschemotherapydrug resistancehyperthermiaTaxanesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4771-4779 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	iron oxide nanoparticles chemotherapy drug resistance hyperthermia Taxanes Medicine (General) R5-920
spellingShingle	iron oxide nanoparticles chemotherapy drug resistance hyperthermia Taxanes Medicine (General) R5-920 Rivera-Rodriguez A Chiu-Lam A Morozov VM Ishov AM Rinaldi C Magnetic nanoparticle hyperthermia potentiates paclitaxel activity in sensitive and resistant breast cancer cells
description	Angelie Rivera-Rodriguez,1 Andreina Chiu-Lam,2 Viacheslav M Morozov,3,4 Alexander M Ishov,3,4 Carlos Rinaldi1,2,4 1J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA; 2Department of Chemical Engineering, University of Florida, Gainesville, FL, USA; 3Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL, USA; 4UF Health Cancer Center Gainesville, FL, USA Introduction: Overcoming resistance to antimitotic drugs, such as paclitaxel (PTX), would represent a major advance in breast cancer treatment. PTX induces mitotic block and sensitive cells exit mitosis dying by mitotic catastrophe. Resistant cells remain in block and continue proliferation after drug decay, denoting one of the PTX resistance mechanisms. Mild hyperthermia (HT) triggers mitotic exit of PTX-pretreated cells, overcoming PTX resistance and suggesting HT-forced mitotic exit as a promising strategy to potentiate PTX. Methods and results: Superparamagnetic iron oxide nanoparticles (SPIONs) were used to deliver mild HT at 42°C in PTX-pretreated breast adenocarcinoma MCF-7 cells sensitive and resistant to PTX. To evaluate mechanism of cell death, cells were classified based on nuclear morphology into interphase, mitotic, micronucleated, and apoptotic. The combined PTX→SPION treatment resulted in an increase in the percentage of micronucleated cells, an indication of forced mitotic exit. Importantly, in PTX-resistant cells, the combination therapy using SPION HT helps to overcome resistance by reducing the number of cells relative to the control. Conclusion: SPION HT potentiates PTX by significantly reducing cell survival, suggesting potential of combined treatment for future clinical translation. Keywords: iron oxide nanoparticles, chemotherapy, drug resistance, hyperthermia, taxanes
format	article
author	Rivera-Rodriguez A Chiu-Lam A Morozov VM Ishov AM Rinaldi C
author_facet	Rivera-Rodriguez A Chiu-Lam A Morozov VM Ishov AM Rinaldi C
author_sort	Rivera-Rodriguez A
title	Magnetic nanoparticle hyperthermia potentiates paclitaxel activity in sensitive and resistant breast cancer cells
title_short	Magnetic nanoparticle hyperthermia potentiates paclitaxel activity in sensitive and resistant breast cancer cells
title_full	Magnetic nanoparticle hyperthermia potentiates paclitaxel activity in sensitive and resistant breast cancer cells
title_fullStr	Magnetic nanoparticle hyperthermia potentiates paclitaxel activity in sensitive and resistant breast cancer cells
title_full_unstemmed	Magnetic nanoparticle hyperthermia potentiates paclitaxel activity in sensitive and resistant breast cancer cells
title_sort	magnetic nanoparticle hyperthermia potentiates paclitaxel activity in sensitive and resistant breast cancer cells
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/3d92dd58f116410c96da167e45e0cde3
work_keys_str_mv	AT riverarodrigueza magneticnanoparticlehyperthermiapotentiatespaclitaxelactivityinsensitiveandresistantbreastcancercells AT chiulama magneticnanoparticlehyperthermiapotentiatespaclitaxelactivityinsensitiveandresistantbreastcancercells AT morozovvm magneticnanoparticlehyperthermiapotentiatespaclitaxelactivityinsensitiveandresistantbreastcancercells AT ishovam magneticnanoparticlehyperthermiapotentiatespaclitaxelactivityinsensitiveandresistantbreastcancercells AT rinaldic magneticnanoparticlehyperthermiapotentiatespaclitaxelactivityinsensitiveandresistantbreastcancercells
_version_	1718399685618565120

Magnetic nanoparticle hyperthermia potentiates paclitaxel activity in sensitive and resistant breast cancer cells

Similar Items