Compromised dental cells viability following teeth-whitening exposure

Abstract This study aimed to assess the viability of dental cells following time-dependent carbamide peroxide teeth-whitening treatments using an in-vitro dentin perfusion assay model. 30 teeth were exposed to 5% or 16% CP gel (4 h daily) for 2-weeks. The enamel organic content was measured with the...

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Autores principales:	Ola Redha, Morteza Mazinanian, Sabrina Nguyen, Dong Ok Son, Monika Lodyga, Boris Hinz, Marianne Odlyha, Ailbhe McDonald, Laurent Bozec
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3d9876734b234b128f12ac9ecadc35ed
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spelling	oai:doaj.org-article:3d9876734b234b128f12ac9ecadc35ed2021-12-02T18:46:54ZCompromised dental cells viability following teeth-whitening exposure10.1038/s41598-021-94745-w2045-2322https://doaj.org/article/3d9876734b234b128f12ac9ecadc35ed2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94745-whttps://doaj.org/toc/2045-2322Abstract This study aimed to assess the viability of dental cells following time-dependent carbamide peroxide teeth-whitening treatments using an in-vitro dentin perfusion assay model. 30 teeth were exposed to 5% or 16% CP gel (4 h daily) for 2-weeks. The enamel organic content was measured with thermogravimetry. The time-dependent viability of human dental pulp stem cells (HDPSCs) and gingival fibroblast cells (HGFCs) following either indirect exposure to 3 commercially available concentrations of CP gel using an in-vitro dentin perfusion assay or direct exposure to 5% H2O2 were investigated by evaluating change in cell morphology and by hemocytometry. The 5% and 16% CP produced a significantly lower (p < 0.001) enamel protein content (by weight) when compared to the control. The organic content in enamel varied accordingly to the CP treatment: for the 16% and 5% CP treatment groups, a variation of 4.0% and 5.4%, respectively, was observed with no significant difference. The cell viability of HDPSCs decreased exponentially over time for all groups. Within the limitation of this in-vitro study, we conclude that even low concentrations of H2O2 and CP result in a deleterious change in enamel protein content and compromise the viability of HGFCs and HDPSCs. These effects should be observed in-vivo.Ola RedhaMorteza MazinanianSabrina NguyenDong Ok SonMonika LodygaBoris HinzMarianne OdlyhaAilbhe McDonaldLaurent BozecNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ola Redha Morteza Mazinanian Sabrina Nguyen Dong Ok Son Monika Lodyga Boris Hinz Marianne Odlyha Ailbhe McDonald Laurent Bozec Compromised dental cells viability following teeth-whitening exposure
description	Abstract This study aimed to assess the viability of dental cells following time-dependent carbamide peroxide teeth-whitening treatments using an in-vitro dentin perfusion assay model. 30 teeth were exposed to 5% or 16% CP gel (4 h daily) for 2-weeks. The enamel organic content was measured with thermogravimetry. The time-dependent viability of human dental pulp stem cells (HDPSCs) and gingival fibroblast cells (HGFCs) following either indirect exposure to 3 commercially available concentrations of CP gel using an in-vitro dentin perfusion assay or direct exposure to 5% H2O2 were investigated by evaluating change in cell morphology and by hemocytometry. The 5% and 16% CP produced a significantly lower (p < 0.001) enamel protein content (by weight) when compared to the control. The organic content in enamel varied accordingly to the CP treatment: for the 16% and 5% CP treatment groups, a variation of 4.0% and 5.4%, respectively, was observed with no significant difference. The cell viability of HDPSCs decreased exponentially over time for all groups. Within the limitation of this in-vitro study, we conclude that even low concentrations of H2O2 and CP result in a deleterious change in enamel protein content and compromise the viability of HGFCs and HDPSCs. These effects should be observed in-vivo.
format	article
author	Ola Redha Morteza Mazinanian Sabrina Nguyen Dong Ok Son Monika Lodyga Boris Hinz Marianne Odlyha Ailbhe McDonald Laurent Bozec
author_facet	Ola Redha Morteza Mazinanian Sabrina Nguyen Dong Ok Son Monika Lodyga Boris Hinz Marianne Odlyha Ailbhe McDonald Laurent Bozec
author_sort	Ola Redha
title	Compromised dental cells viability following teeth-whitening exposure
title_short	Compromised dental cells viability following teeth-whitening exposure
title_full	Compromised dental cells viability following teeth-whitening exposure
title_fullStr	Compromised dental cells viability following teeth-whitening exposure
title_full_unstemmed	Compromised dental cells viability following teeth-whitening exposure
title_sort	compromised dental cells viability following teeth-whitening exposure
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3d9876734b234b128f12ac9ecadc35ed
work_keys_str_mv	AT olaredha compromiseddentalcellsviabilityfollowingteethwhiteningexposure AT mortezamazinanian compromiseddentalcellsviabilityfollowingteethwhiteningexposure AT sabrinanguyen compromiseddentalcellsviabilityfollowingteethwhiteningexposure AT dongokson compromiseddentalcellsviabilityfollowingteethwhiteningexposure AT monikalodyga compromiseddentalcellsviabilityfollowingteethwhiteningexposure AT borishinz compromiseddentalcellsviabilityfollowingteethwhiteningexposure AT marianneodlyha compromiseddentalcellsviabilityfollowingteethwhiteningexposure AT ailbhemcdonald compromiseddentalcellsviabilityfollowingteethwhiteningexposure AT laurentbozec compromiseddentalcellsviabilityfollowingteethwhiteningexposure
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Compromised dental cells viability following teeth-whitening exposure

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