Transcriptomic Profile of the Cockle <i>Cerastoderma edule</i> Exposed to Seasonal Diarrhetic Shellfish Toxin Contamination

Bivalves constitute an important source of proteins for human consumption, but some accumulate biotoxins such as diarrhetic shellfish toxins (DSTs), constituting a risk to human health. The cockle <i>Cerastoderma edule</i> is one of the most important species harvested in the Portuguese...

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Autores principales:	Dany Domínguez-Pérez, José Carlos Martins, Daniela Almeida, Pedro Reis Costa, Vitor Vasconcelos, Alexandre Campos
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	DSTs bivalves RNAseq biomarkers Differentially Expressed Genes (DEGs) Portugal Medicine R
Acceso en línea:	https://doaj.org/article/3d9876e6f4dc443cbcc0a80cc1782a9e
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spelling	oai:doaj.org-article:3d9876e6f4dc443cbcc0a80cc1782a9e2021-11-25T19:08:48ZTranscriptomic Profile of the Cockle <i>Cerastoderma edule</i> Exposed to Seasonal Diarrhetic Shellfish Toxin Contamination10.3390/toxins131107842072-6651https://doaj.org/article/3d9876e6f4dc443cbcc0a80cc1782a9e2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/784https://doaj.org/toc/2072-6651Bivalves constitute an important source of proteins for human consumption, but some accumulate biotoxins such as diarrhetic shellfish toxins (DSTs), constituting a risk to human health. The cockle <i>Cerastoderma edule</i> is one of the most important species harvested in the Portuguese coast but also one of the most affected species due to recurrent DSTs exposure. However, little is known regarding the effects of the toxins produced by blooming dinoflagellates on <i>C. edule</i>. Herein, we explore the Differentially Expressed Genes (DEGs) of two tissues (gills and digestive gland) from wild cockles sampled in Portugal, through their whole transcriptomic response in two different seasons (exposed and not exposed to DSTs). The de novo transcriptome assembly returned 684,723 contigs, N50 of 1049, and 98.53% completeness. Altogether, 1098 DEGs were identified, of which 353 DEGs were exclusive for the digestive gland, 536 unique for the gills and 209 DEGs were common. Among DEGs were identified known DSTs-biomarkers including glutathione peroxidase, glutathione S-transferase, superoxide dismutase, cytochrome P450, ABC transporters, actin and tubulin-related proteins, Heat shock proteins and complement C1Q-like proteins. This study provides the first transcriptomic profile of <i>C. edule</i>, giving new insights about its molecular responses under different environmental conditions of DSTs exposure.Dany Domínguez-PérezJosé Carlos MartinsDaniela AlmeidaPedro Reis CostaVitor VasconcelosAlexandre CamposMDPI AGarticleDSTsbivalvesRNAseqbiomarkersDifferentially Expressed Genes (DEGs)PortugalMedicineRENToxins, Vol 13, Iss 784, p 784 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	DSTs bivalves RNAseq biomarkers Differentially Expressed Genes (DEGs) Portugal Medicine R
spellingShingle	DSTs bivalves RNAseq biomarkers Differentially Expressed Genes (DEGs) Portugal Medicine R Dany Domínguez-Pérez José Carlos Martins Daniela Almeida Pedro Reis Costa Vitor Vasconcelos Alexandre Campos Transcriptomic Profile of the Cockle <i>Cerastoderma edule</i> Exposed to Seasonal Diarrhetic Shellfish Toxin Contamination
description	Bivalves constitute an important source of proteins for human consumption, but some accumulate biotoxins such as diarrhetic shellfish toxins (DSTs), constituting a risk to human health. The cockle <i>Cerastoderma edule</i> is one of the most important species harvested in the Portuguese coast but also one of the most affected species due to recurrent DSTs exposure. However, little is known regarding the effects of the toxins produced by blooming dinoflagellates on <i>C. edule</i>. Herein, we explore the Differentially Expressed Genes (DEGs) of two tissues (gills and digestive gland) from wild cockles sampled in Portugal, through their whole transcriptomic response in two different seasons (exposed and not exposed to DSTs). The de novo transcriptome assembly returned 684,723 contigs, N50 of 1049, and 98.53% completeness. Altogether, 1098 DEGs were identified, of which 353 DEGs were exclusive for the digestive gland, 536 unique for the gills and 209 DEGs were common. Among DEGs were identified known DSTs-biomarkers including glutathione peroxidase, glutathione S-transferase, superoxide dismutase, cytochrome P450, ABC transporters, actin and tubulin-related proteins, Heat shock proteins and complement C1Q-like proteins. This study provides the first transcriptomic profile of <i>C. edule</i>, giving new insights about its molecular responses under different environmental conditions of DSTs exposure.
format	article
author	Dany Domínguez-Pérez José Carlos Martins Daniela Almeida Pedro Reis Costa Vitor Vasconcelos Alexandre Campos
author_facet	Dany Domínguez-Pérez José Carlos Martins Daniela Almeida Pedro Reis Costa Vitor Vasconcelos Alexandre Campos
author_sort	Dany Domínguez-Pérez
title	Transcriptomic Profile of the Cockle <i>Cerastoderma edule</i> Exposed to Seasonal Diarrhetic Shellfish Toxin Contamination
title_short	Transcriptomic Profile of the Cockle <i>Cerastoderma edule</i> Exposed to Seasonal Diarrhetic Shellfish Toxin Contamination
title_full	Transcriptomic Profile of the Cockle <i>Cerastoderma edule</i> Exposed to Seasonal Diarrhetic Shellfish Toxin Contamination
title_fullStr	Transcriptomic Profile of the Cockle <i>Cerastoderma edule</i> Exposed to Seasonal Diarrhetic Shellfish Toxin Contamination
title_full_unstemmed	Transcriptomic Profile of the Cockle <i>Cerastoderma edule</i> Exposed to Seasonal Diarrhetic Shellfish Toxin Contamination
title_sort	transcriptomic profile of the cockle <i>cerastoderma edule</i> exposed to seasonal diarrhetic shellfish toxin contamination
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3d9876e6f4dc443cbcc0a80cc1782a9e
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Transcriptomic Profile of the Cockle <i>Cerastoderma edule</i> Exposed to Seasonal Diarrhetic Shellfish Toxin Contamination

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