Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations

We aimed to evaluate the anticancer potential of crude venom (CV), γ irradiated <i>Certastes cerastes</i> venom (IRRV), and propolis ethanolic extract (PEE). IRRV showed a higher toxicity than CV, while CV-PEE showed higher toxicity than IRRV and CV against lung [A549] and prostate [PC3]...

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Autores principales: Mostafa I. Abdelglil, Sanaa O. Abdallah, Mohamed A. El-Desouky, Mohammad Y. Alfaifi, Serag Eldin I. Elbehairi, Aly F. Mohamed
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
PEE
CV
Acceso en línea:https://doaj.org/article/3d9e2803716c44b9ab31423c2a05cc2a
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Sumario:We aimed to evaluate the anticancer potential of crude venom (CV), γ irradiated <i>Certastes cerastes</i> venom (IRRV), and propolis ethanolic extract (PEE). IRRV showed a higher toxicity than CV, while CV-PEE showed higher toxicity than IRRV and CV against lung [A549] and prostate [PC3] cancer cells. Toxicity to [A549] and [PC3] cells was concentration and cell type dependent. In comparison to controls, apoptotic genes showed a significant upregulation of P53 and Casp-3 and a downregulation of Bcl-2. Also, induced elevated DNA accumulation in the [S] phase post PC3 cell treatment with IRRV and CV, as well as a significant DNA accumulation at G2/M phase after IRRV treatment of A549 cells. In contrast, PC3 cells showed a negligible cellular DNA accumulation after PEE treatment. Glutathione reductase [GR] was reduced in case of PC3 and A549 cell treated with IRRV, CV, and PEE compared with its values in untreated cell control. The Malondialdehyde [MDA] values in both cells recorded a significant elevation post IRRV treatment compared to the rest of the treatment regimen and untreated cell control. Similarly, IRRV and CV-PEE mix showed obviously higher reactive oxygen species [ROS] values than PC3 and A549 cell treatments with CV and PEE.