Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations

We aimed to evaluate the anticancer potential of crude venom (CV), γ irradiated <i>Certastes cerastes</i> venom (IRRV), and propolis ethanolic extract (PEE). IRRV showed a higher toxicity than CV, while CV-PEE showed higher toxicity than IRRV and CV against lung [A549] and prostate [PC3]...

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Autores principales:	Mostafa I. Abdelglil, Sanaa O. Abdallah, Mohamed A. El-Desouky, Mohammad Y. Alfaifi, Serag Eldin I. Elbehairi, Aly F. Mohamed
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	PEE IRR V A549, PC3 apoptotic IC<sub>50</sub> CV Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/3d9e2803716c44b9ab31423c2a05cc2a
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spelling	oai:doaj.org-article:3d9e2803716c44b9ab31423c2a05cc2a2021-11-25T18:29:31ZEvaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations10.3390/molecules262270571420-3049https://doaj.org/article/3d9e2803716c44b9ab31423c2a05cc2a2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/7057https://doaj.org/toc/1420-3049We aimed to evaluate the anticancer potential of crude venom (CV), γ irradiated <i>Certastes cerastes</i> venom (IRRV), and propolis ethanolic extract (PEE). IRRV showed a higher toxicity than CV, while CV-PEE showed higher toxicity than IRRV and CV against lung [A549] and prostate [PC3] cancer cells. Toxicity to [A549] and [PC3] cells was concentration and cell type dependent. In comparison to controls, apoptotic genes showed a significant upregulation of P53 and Casp-3 and a downregulation of Bcl-2. Also, induced elevated DNA accumulation in the [S] phase post PC3 cell treatment with IRRV and CV, as well as a significant DNA accumulation at G2/M phase after IRRV treatment of A549 cells. In contrast, PC3 cells showed a negligible cellular DNA accumulation after PEE treatment. Glutathione reductase [GR] was reduced in case of PC3 and A549 cell treated with IRRV, CV, and PEE compared with its values in untreated cell control. The Malondialdehyde [MDA] values in both cells recorded a significant elevation post IRRV treatment compared to the rest of the treatment regimen and untreated cell control. Similarly, IRRV and CV-PEE mix showed obviously higher reactive oxygen species [ROS] values than PC3 and A549 cell treatments with CV and PEE.Mostafa I. AbdelglilSanaa O. AbdallahMohamed A. El-DesoukyMohammad Y. AlfaifiSerag Eldin I. ElbehairiAly F. MohamedMDPI AGarticlePEEIRR VA549, PC3apoptoticIC<sub>50</sub>CVOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 7057, p 7057 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PEE IRR V A549, PC3 apoptotic IC<sub>50</sub> CV Organic chemistry QD241-441
spellingShingle	PEE IRR V A549, PC3 apoptotic IC<sub>50</sub> CV Organic chemistry QD241-441 Mostafa I. Abdelglil Sanaa O. Abdallah Mohamed A. El-Desouky Mohammad Y. Alfaifi Serag Eldin I. Elbehairi Aly F. Mohamed Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations
description	We aimed to evaluate the anticancer potential of crude venom (CV), γ irradiated <i>Certastes cerastes</i> venom (IRRV), and propolis ethanolic extract (PEE). IRRV showed a higher toxicity than CV, while CV-PEE showed higher toxicity than IRRV and CV against lung [A549] and prostate [PC3] cancer cells. Toxicity to [A549] and [PC3] cells was concentration and cell type dependent. In comparison to controls, apoptotic genes showed a significant upregulation of P53 and Casp-3 and a downregulation of Bcl-2. Also, induced elevated DNA accumulation in the [S] phase post PC3 cell treatment with IRRV and CV, as well as a significant DNA accumulation at G2/M phase after IRRV treatment of A549 cells. In contrast, PC3 cells showed a negligible cellular DNA accumulation after PEE treatment. Glutathione reductase [GR] was reduced in case of PC3 and A549 cell treated with IRRV, CV, and PEE compared with its values in untreated cell control. The Malondialdehyde [MDA] values in both cells recorded a significant elevation post IRRV treatment compared to the rest of the treatment regimen and untreated cell control. Similarly, IRRV and CV-PEE mix showed obviously higher reactive oxygen species [ROS] values than PC3 and A549 cell treatments with CV and PEE.
format	article
author	Mostafa I. Abdelglil Sanaa O. Abdallah Mohamed A. El-Desouky Mohammad Y. Alfaifi Serag Eldin I. Elbehairi Aly F. Mohamed
author_facet	Mostafa I. Abdelglil Sanaa O. Abdallah Mohamed A. El-Desouky Mohammad Y. Alfaifi Serag Eldin I. Elbehairi Aly F. Mohamed
author_sort	Mostafa I. Abdelglil
title	Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations
title_short	Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations
title_full	Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations
title_fullStr	Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations
title_full_unstemmed	Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations
title_sort	evaluation of the anticancer potential of crude, irradiated <i>cerastes cerastes</i> snake venom and propolis ethanolic extract & related biological alterations
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3d9e2803716c44b9ab31423c2a05cc2a
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Evaluation of the Anticancer Potential of Crude, Irradiated <i>Cerastes cerastes</i> Snake Venom and Propolis Ethanolic Extract & Related Biological Alterations

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