Comprehensive analysis of competitive endogenous RNA associated with immune infiltration in lung adenocarcinoma

Abstract To identify the prognostic biomarker of the competitive endogenous RNA (ceRNA) and explore the tumor infiltrating immune cells (TIICs) which might be the potential prognostic factors in lung adenocarcinoma. In addition, we also try to explain the crosstalk between the ceRNA and TIICs to exp...

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Autores principales:	Wenjie Chen, Wen Li, Zhenkun Liu, Guangzhi Ma, Yunfu Deng, Xiaogang Li, Zhu Wang, Qinghua zhou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3d9ebf6a06744f4e9027ffbdbbcdf30a
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spelling	oai:doaj.org-article:3d9ebf6a06744f4e9027ffbdbbcdf30a2021-12-02T15:49:42ZComprehensive analysis of competitive endogenous RNA associated with immune infiltration in lung adenocarcinoma10.1038/s41598-021-90755-w2045-2322https://doaj.org/article/3d9ebf6a06744f4e9027ffbdbbcdf30a2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90755-whttps://doaj.org/toc/2045-2322Abstract To identify the prognostic biomarker of the competitive endogenous RNA (ceRNA) and explore the tumor infiltrating immune cells (TIICs) which might be the potential prognostic factors in lung adenocarcinoma. In addition, we also try to explain the crosstalk between the ceRNA and TIICs to explore the molecular mechanisms involved in lung adenocarcinoma. The transcriptome data of lung adenocarcinoma were obtained from The Cancer Genome Atlas (TCGA) database, and the hypergeometric correlation of the differently expressed miRNA-lncRNA and miRNA-mRNA were analyzed based on the starBase. In addition, the Kaplan–Meier survival and Cox regression model analysis were used to identify the prognostic ceRNA network and TIICs. Correlation analysis was performed to analysis the correlation between the ceRNA network and TIICs. In the differently expressed RNAs between tumor and normal tissue, a total of 190 miRNAs, 224 lncRNAs and 3024 mRNAs were detected, and the constructed ceRNA network contained 5 lncRNAs, 92 mRNAs and 10 miRNAs. Then, six prognostic RNAs (FKBP3, GPI, LOXL2, IL22RA1, GPR37, and has-miR-148a-3p) were viewed as the key members for constructing the prognostic prediction model in the ceRNA network, and three kinds of TIICs (Monocytes, Macrophages M1, activated mast cells) were identified to be significantly related with the prognosis in lung adenocarcinoma. Correlation analysis suggested that the FKBP3 was associated with Monocytes and Macrophages M1, and the GPI was obviously related with Monocytes and Macrophages M1. Besides, the LOXL2 was associated with Monocytes and Activated mast cells, and the IL22RA1 was significantly associated with Monocytes and Macrophages M1, while the GPR37 and Macrophages M1 was closely related. The constructed ceRNA network and identified Monocytes, Macrophages M1 and activated Mast cells are all prognostic factors for lung adenocarcinoma. Moreover, the crosstalk between the ceRNA network and TIICs might be a potential molecular mechanism involved.Wenjie ChenWen LiZhenkun LiuGuangzhi MaYunfu DengXiaogang LiZhu WangQinghua zhouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenjie Chen Wen Li Zhenkun Liu Guangzhi Ma Yunfu Deng Xiaogang Li Zhu Wang Qinghua zhou Comprehensive analysis of competitive endogenous RNA associated with immune infiltration in lung adenocarcinoma
description	Abstract To identify the prognostic biomarker of the competitive endogenous RNA (ceRNA) and explore the tumor infiltrating immune cells (TIICs) which might be the potential prognostic factors in lung adenocarcinoma. In addition, we also try to explain the crosstalk between the ceRNA and TIICs to explore the molecular mechanisms involved in lung adenocarcinoma. The transcriptome data of lung adenocarcinoma were obtained from The Cancer Genome Atlas (TCGA) database, and the hypergeometric correlation of the differently expressed miRNA-lncRNA and miRNA-mRNA were analyzed based on the starBase. In addition, the Kaplan–Meier survival and Cox regression model analysis were used to identify the prognostic ceRNA network and TIICs. Correlation analysis was performed to analysis the correlation between the ceRNA network and TIICs. In the differently expressed RNAs between tumor and normal tissue, a total of 190 miRNAs, 224 lncRNAs and 3024 mRNAs were detected, and the constructed ceRNA network contained 5 lncRNAs, 92 mRNAs and 10 miRNAs. Then, six prognostic RNAs (FKBP3, GPI, LOXL2, IL22RA1, GPR37, and has-miR-148a-3p) were viewed as the key members for constructing the prognostic prediction model in the ceRNA network, and three kinds of TIICs (Monocytes, Macrophages M1, activated mast cells) were identified to be significantly related with the prognosis in lung adenocarcinoma. Correlation analysis suggested that the FKBP3 was associated with Monocytes and Macrophages M1, and the GPI was obviously related with Monocytes and Macrophages M1. Besides, the LOXL2 was associated with Monocytes and Activated mast cells, and the IL22RA1 was significantly associated with Monocytes and Macrophages M1, while the GPR37 and Macrophages M1 was closely related. The constructed ceRNA network and identified Monocytes, Macrophages M1 and activated Mast cells are all prognostic factors for lung adenocarcinoma. Moreover, the crosstalk between the ceRNA network and TIICs might be a potential molecular mechanism involved.
format	article
author	Wenjie Chen Wen Li Zhenkun Liu Guangzhi Ma Yunfu Deng Xiaogang Li Zhu Wang Qinghua zhou
author_facet	Wenjie Chen Wen Li Zhenkun Liu Guangzhi Ma Yunfu Deng Xiaogang Li Zhu Wang Qinghua zhou
author_sort	Wenjie Chen
title	Comprehensive analysis of competitive endogenous RNA associated with immune infiltration in lung adenocarcinoma
title_short	Comprehensive analysis of competitive endogenous RNA associated with immune infiltration in lung adenocarcinoma
title_full	Comprehensive analysis of competitive endogenous RNA associated with immune infiltration in lung adenocarcinoma
title_fullStr	Comprehensive analysis of competitive endogenous RNA associated with immune infiltration in lung adenocarcinoma
title_full_unstemmed	Comprehensive analysis of competitive endogenous RNA associated with immune infiltration in lung adenocarcinoma
title_sort	comprehensive analysis of competitive endogenous rna associated with immune infiltration in lung adenocarcinoma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3d9ebf6a06744f4e9027ffbdbbcdf30a
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_version_	1718385684700463104

Comprehensive analysis of competitive endogenous RNA associated with immune infiltration in lung adenocarcinoma

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