Expedient assessment of post-infarct remodeling by native cardiac magnetic resonance imaging in mice

Abstract Novel therapeutic strategies aiming at improving the healing process after an acute myocardial infarction are currently under intense investigation. The mouse model plays a central role for deciphering the underlying mechanisms on a molecular and cellular level. Therefore, we intended to as...

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Autores principales:	Cajetan Immanuel Lang, Praveen Vasudevan, Piet Döring, Ralf Gäbel, Heiko Lemcke, Tobias Lindner, Gustav Steinhoff, Bernd Joachim Krause, Brigitte Vollmar, Felix G. Meinel, Seyrani Yücel, Alper Öner, Hüseyin Ince, Robert David
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3da395c736904befa2a3897a682bd2c2
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spelling	oai:doaj.org-article:3da395c736904befa2a3897a682bd2c22021-12-02T15:02:48ZExpedient assessment of post-infarct remodeling by native cardiac magnetic resonance imaging in mice10.1038/s41598-021-91096-42045-2322https://doaj.org/article/3da395c736904befa2a3897a682bd2c22021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91096-4https://doaj.org/toc/2045-2322Abstract Novel therapeutic strategies aiming at improving the healing process after an acute myocardial infarction are currently under intense investigation. The mouse model plays a central role for deciphering the underlying mechanisms on a molecular and cellular level. Therefore, we intended to assess in-vivo post-infarct remodeling as comprehensively as possible using an expedient native magnetic resonance imaging (MRI) in the two most prominent infarct models, permanent ligation (PL) of the left anterior descending artery (LAD) versus ischemia reperfusion (I/R). Mice were subjected to either permanent or transient (45 min) occlusion of the LAD. After 3 weeks, examinations were performed with a 7-Tesla small animal MRI system. Data analysis was performed with the freely available software Segment. PL resulted in a massive dilation of the left ventricle, accompanied by hypertrophy of the non-infarcted myocardium and a decline of contractile function. These effects were less pronounced following I/R compared to healthy animals. Single plane assessments were not sufficient to capture the specific differences of left ventricular (LV) properties between the two infarct models. Bulls-eye plots were found to be an ideal tool for qualitative LV wall assessment, whereas a multi-slice sector-based analysis of wall regions is ideal to determine differences in hypertrophy, lateral wall thinning and wall thickening on a quantitative level. We combine the use of polar map-based analysis of LV wall properties with volumetric measurements using simple CINE CMR imaging. Our strategy represents a versatile and easily available tool for serial assessment of the LV during the remodeling process. Our study contributes to a better understanding of the effects of novel therapies targeting the healing of damaged myocardium.Cajetan Immanuel LangPraveen VasudevanPiet DöringRalf GäbelHeiko LemckeTobias LindnerGustav SteinhoffBernd Joachim KrauseBrigitte VollmarFelix G. MeinelSeyrani YücelAlper ÖnerHüseyin InceRobert DavidNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cajetan Immanuel Lang Praveen Vasudevan Piet Döring Ralf Gäbel Heiko Lemcke Tobias Lindner Gustav Steinhoff Bernd Joachim Krause Brigitte Vollmar Felix G. Meinel Seyrani Yücel Alper Öner Hüseyin Ince Robert David Expedient assessment of post-infarct remodeling by native cardiac magnetic resonance imaging in mice
description	Abstract Novel therapeutic strategies aiming at improving the healing process after an acute myocardial infarction are currently under intense investigation. The mouse model plays a central role for deciphering the underlying mechanisms on a molecular and cellular level. Therefore, we intended to assess in-vivo post-infarct remodeling as comprehensively as possible using an expedient native magnetic resonance imaging (MRI) in the two most prominent infarct models, permanent ligation (PL) of the left anterior descending artery (LAD) versus ischemia reperfusion (I/R). Mice were subjected to either permanent or transient (45 min) occlusion of the LAD. After 3 weeks, examinations were performed with a 7-Tesla small animal MRI system. Data analysis was performed with the freely available software Segment. PL resulted in a massive dilation of the left ventricle, accompanied by hypertrophy of the non-infarcted myocardium and a decline of contractile function. These effects were less pronounced following I/R compared to healthy animals. Single plane assessments were not sufficient to capture the specific differences of left ventricular (LV) properties between the two infarct models. Bulls-eye plots were found to be an ideal tool for qualitative LV wall assessment, whereas a multi-slice sector-based analysis of wall regions is ideal to determine differences in hypertrophy, lateral wall thinning and wall thickening on a quantitative level. We combine the use of polar map-based analysis of LV wall properties with volumetric measurements using simple CINE CMR imaging. Our strategy represents a versatile and easily available tool for serial assessment of the LV during the remodeling process. Our study contributes to a better understanding of the effects of novel therapies targeting the healing of damaged myocardium.
format	article
author	Cajetan Immanuel Lang Praveen Vasudevan Piet Döring Ralf Gäbel Heiko Lemcke Tobias Lindner Gustav Steinhoff Bernd Joachim Krause Brigitte Vollmar Felix G. Meinel Seyrani Yücel Alper Öner Hüseyin Ince Robert David
author_facet	Cajetan Immanuel Lang Praveen Vasudevan Piet Döring Ralf Gäbel Heiko Lemcke Tobias Lindner Gustav Steinhoff Bernd Joachim Krause Brigitte Vollmar Felix G. Meinel Seyrani Yücel Alper Öner Hüseyin Ince Robert David
author_sort	Cajetan Immanuel Lang
title	Expedient assessment of post-infarct remodeling by native cardiac magnetic resonance imaging in mice
title_short	Expedient assessment of post-infarct remodeling by native cardiac magnetic resonance imaging in mice
title_full	Expedient assessment of post-infarct remodeling by native cardiac magnetic resonance imaging in mice
title_fullStr	Expedient assessment of post-infarct remodeling by native cardiac magnetic resonance imaging in mice
title_full_unstemmed	Expedient assessment of post-infarct remodeling by native cardiac magnetic resonance imaging in mice
title_sort	expedient assessment of post-infarct remodeling by native cardiac magnetic resonance imaging in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3da395c736904befa2a3897a682bd2c2
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Expedient assessment of post-infarct remodeling by native cardiac magnetic resonance imaging in mice

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