Oral Immunization with Cholera Toxin Provides Protection against <named-content content-type="genus-species">Campylobacter jejuni</named-content> in an Adult Mouse Intestinal Colonization Model

ABSTRACT Immunity to Campylobacter jejuni, a major diarrheal pathogen, is largely Penner serotype specific. For broad protection, a vaccine should be based on a common antigen(s) present in all strains. In our previous study (M. J. Albert, S. Haridas, D. Steer, G. S. Dhaunsi, A. I. Smith, and B. Adl...

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Autores principales:	M. John Albert, Abu Salim Mustafa, Anjum Islam, Shilpa Haridas
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3da815b86fe0478a95905565b09ecf10
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spelling	oai:doaj.org-article:3da815b86fe0478a95905565b09ecf102021-11-15T15:40:05ZOral Immunization with Cholera Toxin Provides Protection against <named-content content-type="genus-species">Campylobacter jejuni</named-content> in an Adult Mouse Intestinal Colonization Model10.1128/mBio.00246-132150-7511https://doaj.org/article/3da815b86fe0478a95905565b09ecf102013-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00246-13https://doaj.org/toc/2150-7511ABSTRACT Immunity to Campylobacter jejuni, a major diarrheal pathogen, is largely Penner serotype specific. For broad protection, a vaccine should be based on a common antigen(s) present in all strains. In our previous study (M. J. Albert, S. Haridas, D. Steer, G. S. Dhaunsi, A. I. Smith, and B. Adler, Infect. Immun. 75:3070–3073, 2007), we demonstrated that antibody to cholera toxin (CT) cross-reacted with the major outer membrane proteins (MOMPs) of all Campylobacter jejuni strains tested. In the current study, we investigated whether immunization with CT protects against intestinal colonization by C. jejuni in an adult mouse model and whether the nontoxic subunit of CT (CT-B) is the portion mediating cross-reaction. Mice were orally immunized with CT and later challenged with C. jejuni strains (48, 75, and 111) of different serotypes. Control animals were immunized with phosphate-buffered saline. Fecal shedding of challenge organisms was studied daily for 9 days. Serum and fecal antibody responses were studied by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. The cross-reactivity of rabbit CT-B antibody to MOMP was studied by immunoblotting. The reactivity of 21 overlapping 30-mer oligopeptides (based on MOMP’s sequence) against rabbit CT antibody was tested by ELISA. Test animals produced antibodies to CT and MMP in serum and feces and showed resistance to colonization, the vaccine efficacies being 49% (for strain 48), 37% (for strain 75), and 34% (for strain 111) (P, ≤0.05 to ≤0.001). One peptide corresponding to a variable region of MOMP showed significant reactivity. CT-B antibody cross-reacted with MOMP. Since CT-B is a component of oral cholera vaccines, it might be possible to control C. jejuni diarrhea with these vaccines. IMPORTANCE Campylobacter jejuni is a major cause of diarrhea worldwide. Patients who recover from C. jejuni diarrhea develop immunity to the infecting serotype and remain susceptible to infection with other serotypes. A vaccine based on a common protective antigen(s) present in all C. jejuni serotypes is expected to provide broad protection. In our previous study, we showed that antibody to cholera toxin (CT) reacted with the major outer membrane proteins (MOMPs) from different strains of C. jejuni. We assumed that the B subunit of the toxin (CT-B), which is nontoxic and a component of licensed oral cholera vaccines, might be the component that cross-reacts with MOMP. In the current study, we showed that orally immunizing mice with CT protected them against colonization upon challenge with different serotypes of C. jejuni. We also showed that CT-B is the component mediating cross-reaction. Therefore, it might be possible to use cholera vaccines to prevent C. jejuni diarrhea. This could result in significant savings in vaccine development and treatment of the disease.M. John AlbertAbu Salim MustafaAnjum IslamShilpa HaridasAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 3 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 M. John Albert Abu Salim Mustafa Anjum Islam Shilpa Haridas Oral Immunization with Cholera Toxin Provides Protection against <named-content content-type="genus-species">Campylobacter jejuni</named-content> in an Adult Mouse Intestinal Colonization Model
description	ABSTRACT Immunity to Campylobacter jejuni, a major diarrheal pathogen, is largely Penner serotype specific. For broad protection, a vaccine should be based on a common antigen(s) present in all strains. In our previous study (M. J. Albert, S. Haridas, D. Steer, G. S. Dhaunsi, A. I. Smith, and B. Adler, Infect. Immun. 75:3070–3073, 2007), we demonstrated that antibody to cholera toxin (CT) cross-reacted with the major outer membrane proteins (MOMPs) of all Campylobacter jejuni strains tested. In the current study, we investigated whether immunization with CT protects against intestinal colonization by C. jejuni in an adult mouse model and whether the nontoxic subunit of CT (CT-B) is the portion mediating cross-reaction. Mice were orally immunized with CT and later challenged with C. jejuni strains (48, 75, and 111) of different serotypes. Control animals were immunized with phosphate-buffered saline. Fecal shedding of challenge organisms was studied daily for 9 days. Serum and fecal antibody responses were studied by enzyme-linked immunosorbent assay (ELISA) and immunoblotting. The cross-reactivity of rabbit CT-B antibody to MOMP was studied by immunoblotting. The reactivity of 21 overlapping 30-mer oligopeptides (based on MOMP’s sequence) against rabbit CT antibody was tested by ELISA. Test animals produced antibodies to CT and MMP in serum and feces and showed resistance to colonization, the vaccine efficacies being 49% (for strain 48), 37% (for strain 75), and 34% (for strain 111) (P, ≤0.05 to ≤0.001). One peptide corresponding to a variable region of MOMP showed significant reactivity. CT-B antibody cross-reacted with MOMP. Since CT-B is a component of oral cholera vaccines, it might be possible to control C. jejuni diarrhea with these vaccines. IMPORTANCE Campylobacter jejuni is a major cause of diarrhea worldwide. Patients who recover from C. jejuni diarrhea develop immunity to the infecting serotype and remain susceptible to infection with other serotypes. A vaccine based on a common protective antigen(s) present in all C. jejuni serotypes is expected to provide broad protection. In our previous study, we showed that antibody to cholera toxin (CT) reacted with the major outer membrane proteins (MOMPs) from different strains of C. jejuni. We assumed that the B subunit of the toxin (CT-B), which is nontoxic and a component of licensed oral cholera vaccines, might be the component that cross-reacts with MOMP. In the current study, we showed that orally immunizing mice with CT protected them against colonization upon challenge with different serotypes of C. jejuni. We also showed that CT-B is the component mediating cross-reaction. Therefore, it might be possible to use cholera vaccines to prevent C. jejuni diarrhea. This could result in significant savings in vaccine development and treatment of the disease.
format	article
author	M. John Albert Abu Salim Mustafa Anjum Islam Shilpa Haridas
author_facet	M. John Albert Abu Salim Mustafa Anjum Islam Shilpa Haridas
author_sort	M. John Albert
title	Oral Immunization with Cholera Toxin Provides Protection against <named-content content-type="genus-species">Campylobacter jejuni</named-content> in an Adult Mouse Intestinal Colonization Model
title_short	Oral Immunization with Cholera Toxin Provides Protection against <named-content content-type="genus-species">Campylobacter jejuni</named-content> in an Adult Mouse Intestinal Colonization Model
title_full	Oral Immunization with Cholera Toxin Provides Protection against <named-content content-type="genus-species">Campylobacter jejuni</named-content> in an Adult Mouse Intestinal Colonization Model
title_fullStr	Oral Immunization with Cholera Toxin Provides Protection against <named-content content-type="genus-species">Campylobacter jejuni</named-content> in an Adult Mouse Intestinal Colonization Model
title_full_unstemmed	Oral Immunization with Cholera Toxin Provides Protection against <named-content content-type="genus-species">Campylobacter jejuni</named-content> in an Adult Mouse Intestinal Colonization Model
title_sort	oral immunization with cholera toxin provides protection against <named-content content-type="genus-species">campylobacter jejuni</named-content> in an adult mouse intestinal colonization model
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/3da815b86fe0478a95905565b09ecf10
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Oral Immunization with Cholera Toxin Provides Protection against <named-content content-type="genus-species">Campylobacter jejuni</named-content> in an Adult Mouse Intestinal Colonization Model

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