Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate

Microfluidics is applied in biotechnology research via the creation of microfluidic channels and reaction vessels. Filters are considered to be able to simulate microfluidics. A typical example is the cell culture insert, which comprises two vessels connected by a filter. Cell culture inserts have b...

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Autores principales:	Takeo Shimasaki, Satoko Yamamoto, Risa Omura, Kagenori Ito, Yumiko Nishide, Hideki Yamada, Kazumi Ohtomo, Tomo Ishisaka, Keiichiro Okano, Takenori Ogawa, Hiroyuki Tsuji, Yoichi Matsuo, Toshinari Minamoto, Naohisa Tomosugi, Etienne Ferain, Takahiro Ochiya
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	co-culture plate multiple connection horizontal co-culture extracellular vesicles exosome track-etched-membrane Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/3db5e14965d9480aa9c15f4763df7c82
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spelling	oai:doaj.org-article:3db5e14965d9480aa9c15f4763df7c822021-11-25T18:23:57ZNovel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate10.3390/mi121114312072-666Xhttps://doaj.org/article/3db5e14965d9480aa9c15f4763df7c822021-11-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1431https://doaj.org/toc/2072-666XMicrofluidics is applied in biotechnology research via the creation of microfluidic channels and reaction vessels. Filters are considered to be able to simulate microfluidics. A typical example is the cell culture insert, which comprises two vessels connected by a filter. Cell culture inserts have been used for years to study cell-to-cell communication. These systems generally have a bucket-in-bucket structure and are hereafter referred to as a vertical-type co-culture plate (VTCP). However, VTCPs have several disadvantages, such as the inability to simultaneously observe samples in both containers and the inability of cell-to-cell communication through the filters at high cell densities. In this study, we developed a novel horizontal-type co-culture plate (HTCP) to overcome these disadvantages and confirm its performance. In addition, we clarified the migration characteristics of substances secreted from cells in horizontal co-culture vessels. It is generally assumed that less material is exchanged between the horizontal vessels. However, the extracellular vesicle (EV) transfer was found to be twice as high when using HTCP. Other merits include control of the degree of co-culture via the placement of cells. We believe that this novel HTCP container will facilitate research on cell-to-cell communication in various fields.Takeo ShimasakiSatoko YamamotoRisa OmuraKagenori ItoYumiko NishideHideki YamadaKazumi OhtomoTomo IshisakaKeiichiro OkanoTakenori OgawaHiroyuki TsujiYoichi MatsuoToshinari MinamotoNaohisa TomosugiEtienne FerainTakahiro OchiyaMDPI AGarticleco-culture platemultiple connectionhorizontal co-cultureextracellular vesiclesexosometrack-etched-membraneMechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1431, p 1431 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	co-culture plate multiple connection horizontal co-culture extracellular vesicles exosome track-etched-membrane Mechanical engineering and machinery TJ1-1570
spellingShingle	co-culture plate multiple connection horizontal co-culture extracellular vesicles exosome track-etched-membrane Mechanical engineering and machinery TJ1-1570 Takeo Shimasaki Satoko Yamamoto Risa Omura Kagenori Ito Yumiko Nishide Hideki Yamada Kazumi Ohtomo Tomo Ishisaka Keiichiro Okano Takenori Ogawa Hiroyuki Tsuji Yoichi Matsuo Toshinari Minamoto Naohisa Tomosugi Etienne Ferain Takahiro Ochiya Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate
description	Microfluidics is applied in biotechnology research via the creation of microfluidic channels and reaction vessels. Filters are considered to be able to simulate microfluidics. A typical example is the cell culture insert, which comprises two vessels connected by a filter. Cell culture inserts have been used for years to study cell-to-cell communication. These systems generally have a bucket-in-bucket structure and are hereafter referred to as a vertical-type co-culture plate (VTCP). However, VTCPs have several disadvantages, such as the inability to simultaneously observe samples in both containers and the inability of cell-to-cell communication through the filters at high cell densities. In this study, we developed a novel horizontal-type co-culture plate (HTCP) to overcome these disadvantages and confirm its performance. In addition, we clarified the migration characteristics of substances secreted from cells in horizontal co-culture vessels. It is generally assumed that less material is exchanged between the horizontal vessels. However, the extracellular vesicle (EV) transfer was found to be twice as high when using HTCP. Other merits include control of the degree of co-culture via the placement of cells. We believe that this novel HTCP container will facilitate research on cell-to-cell communication in various fields.
format	article
author	Takeo Shimasaki Satoko Yamamoto Risa Omura Kagenori Ito Yumiko Nishide Hideki Yamada Kazumi Ohtomo Tomo Ishisaka Keiichiro Okano Takenori Ogawa Hiroyuki Tsuji Yoichi Matsuo Toshinari Minamoto Naohisa Tomosugi Etienne Ferain Takahiro Ochiya
author_facet	Takeo Shimasaki Satoko Yamamoto Risa Omura Kagenori Ito Yumiko Nishide Hideki Yamada Kazumi Ohtomo Tomo Ishisaka Keiichiro Okano Takenori Ogawa Hiroyuki Tsuji Yoichi Matsuo Toshinari Minamoto Naohisa Tomosugi Etienne Ferain Takahiro Ochiya
author_sort	Takeo Shimasaki
title	Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate
title_short	Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate
title_full	Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate
title_fullStr	Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate
title_full_unstemmed	Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate
title_sort	novel platform for regulation of extracellular vesicles and metabolites secretion from cells using a multi-linkable horizontal co-culture plate
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3db5e14965d9480aa9c15f4763df7c82
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Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate

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