Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth

Abstract. Swantara IMD, Bawa IGAG, Suprapta DN, Agustina KK, Temaja IGRM. 2020. Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth. Biodiversitas 21: 1541-1550. Fungicides are substances that inhibit...

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Autores principales: I Made Dira Swantara, I Gusti Agung Gede Bawa, Dewa Ngurah Suprapta, Kadek Karang Agustina, I Gede Rai Maya Temaja
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Publicado: MBI & UNS Solo 2020
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spelling oai:doaj.org-article:3db5fdcd499e469dad835452837f77fa2021-11-22T00:34:39ZIdentification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth1412-033X2085-472210.13057/biodiv/d210435https://doaj.org/article/3db5fdcd499e469dad835452837f77fa2020-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5358https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Swantara IMD, Bawa IGAG, Suprapta DN, Agustina KK, Temaja IGRM. 2020. Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth. Biodiversitas 21: 1541-1550. Fungicides are substances that inhibit the growth or kill the pathogenic fungi. A substance can be categorized as a fungicide if it shows antifungal activity. This activity is resulting from bioactive compounds derived from the secondary metabolites. Michelia alba bark’s extract could inhibit the growth of Curvularia verruculosa, the cause of leaf spot disease on rice. The purpose of this study was to analyze chemical compounds of bark extract which inhibit the growth of C. verruculosa. The extraction of bark metabolites of M. alba was done using methanol as the solvent. The inhibitory test of the extract was carried out by the diffusion well method on Potato Dextrose Agar (PDA) medium. Analysis of chemical compounds of the M. alba extract was carried out in Gas chromatography-mass spectroscopy. The results of inhibitory test found that the M alba bark extract inhibits the C. verruculosa with the inhibition zone as wide as 36 mm in diameter. The majority of chemical compounds identified from the M. alba extract consisted of 10 compounds, namely: Hexadecanoic acid, methyl ester; 4H-tomentosine; 4H-tomentosine (compound isomer 2); 3-hydroxy pregn-4-ene-20-one; Tomentosine; 2-hydroxy tomentosine; Tert-butyl-2-aminophenylcarbamol; 2H-cyclohepta [b] furan-2-one, 3,3a, 4,7,8,8a-hexahydro-7-methyl-3-methylene-6-(2-formil-3-oxobutyl)-; Isoxanthanol and Xanthanol. To conclude, this extract is useful to treat C. verruculosa that causes leaf spot disease on rice.I Made Dira SwantaraI Gusti Agung Gede BawaDewa Ngurah SupraptaKadek Karang AgustinaI Gede Rai Maya TemajaMBI & UNS Soloarticlechemical compounds, curvularia verruculosa, fungicides, gas chromatography, michelia alba, spectrometryBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 4 (2020)
institution DOAJ
collection DOAJ
language EN
topic chemical compounds, curvularia verruculosa, fungicides, gas chromatography, michelia alba, spectrometry
Biology (General)
QH301-705.5
spellingShingle chemical compounds, curvularia verruculosa, fungicides, gas chromatography, michelia alba, spectrometry
Biology (General)
QH301-705.5
I Made Dira Swantara
I Gusti Agung Gede Bawa
Dewa Ngurah Suprapta
Kadek Karang Agustina
I Gede Rai Maya Temaja
Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth
description Abstract. Swantara IMD, Bawa IGAG, Suprapta DN, Agustina KK, Temaja IGRM. 2020. Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth. Biodiversitas 21: 1541-1550. Fungicides are substances that inhibit the growth or kill the pathogenic fungi. A substance can be categorized as a fungicide if it shows antifungal activity. This activity is resulting from bioactive compounds derived from the secondary metabolites. Michelia alba bark’s extract could inhibit the growth of Curvularia verruculosa, the cause of leaf spot disease on rice. The purpose of this study was to analyze chemical compounds of bark extract which inhibit the growth of C. verruculosa. The extraction of bark metabolites of M. alba was done using methanol as the solvent. The inhibitory test of the extract was carried out by the diffusion well method on Potato Dextrose Agar (PDA) medium. Analysis of chemical compounds of the M. alba extract was carried out in Gas chromatography-mass spectroscopy. The results of inhibitory test found that the M alba bark extract inhibits the C. verruculosa with the inhibition zone as wide as 36 mm in diameter. The majority of chemical compounds identified from the M. alba extract consisted of 10 compounds, namely: Hexadecanoic acid, methyl ester; 4H-tomentosine; 4H-tomentosine (compound isomer 2); 3-hydroxy pregn-4-ene-20-one; Tomentosine; 2-hydroxy tomentosine; Tert-butyl-2-aminophenylcarbamol; 2H-cyclohepta [b] furan-2-one, 3,3a, 4,7,8,8a-hexahydro-7-methyl-3-methylene-6-(2-formil-3-oxobutyl)-; Isoxanthanol and Xanthanol. To conclude, this extract is useful to treat C. verruculosa that causes leaf spot disease on rice.
format article
author I Made Dira Swantara
I Gusti Agung Gede Bawa
Dewa Ngurah Suprapta
Kadek Karang Agustina
I Gede Rai Maya Temaja
author_facet I Made Dira Swantara
I Gusti Agung Gede Bawa
Dewa Ngurah Suprapta
Kadek Karang Agustina
I Gede Rai Maya Temaja
author_sort I Made Dira Swantara
title Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth
title_short Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth
title_full Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth
title_fullStr Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth
title_full_unstemmed Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth
title_sort identification michelia alba barks extract using gas chromatography-mass spectrometry (gc-ms) and its antifungal properties to inhibit microbial growth
publisher MBI & UNS Solo
publishDate 2020
url https://doaj.org/article/3db5fdcd499e469dad835452837f77fa
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