Self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil

Narayanan Ashwanikumar,1 Nisha Asok Kumar,2 Padma S Saneesh Babu,2 Krishnankutty C Sivakumar,3 Mithun Varghese Vadakkan,1 Parvathi Nair,1 Ilamathi Hema Saranya,1 Sivakumari Asha Nair,2 Gopalakrishnapillai S Vinod Kumar1 1Chemical Biology, Nano Drug Delivery Systems, Bio-Innovation Center, 2Cancer R...

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Autores principales:	Ashwanikumar N, Kumar NA, Saneesh Babu PS, Sivakumar KC, Vadakkan MV, Nair P, Hema Saranya I, Asha Nair S, Vinod Kumar GS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	scaffold drug delivery nanofibrous aromatic Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3db97da7dc4042c68371541f1c3b655e
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spelling	oai:doaj.org-article:3db97da7dc4042c68371541f1c3b655e2021-12-02T02:08:25ZSelf-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil1178-2013https://doaj.org/article/3db97da7dc4042c68371541f1c3b655e2016-10-01T00:00:00Zhttps://www.dovepress.com/self-assembling-peptide-nanofibers-containing-phenylalanine-for-the-co-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Narayanan Ashwanikumar,1 Nisha Asok Kumar,2 Padma S Saneesh Babu,2 Krishnankutty C Sivakumar,3 Mithun Varghese Vadakkan,1 Parvathi Nair,1 Ilamathi Hema Saranya,1 Sivakumari Asha Nair,2 Gopalakrishnapillai S Vinod Kumar1 1Chemical Biology, Nano Drug Delivery Systems, Bio-Innovation Center, 2Cancer Research Programme, 3Distributed Information Sub-Centre (Bioinformatics Centre), Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India Abstract: The study shows that RADA-F6 peptide with pH-responsive self-assembling nature can be effectively used as a drug delivery system for the sustained release of a potent anticancer drug 5-fluorouracil (5-FU) at basic pH. As 5-FU contains the aromatic pyrimidine ring, RADA-F6 system is suitable for entrapping an aromatic drug due to effective π–π stacking with phenylalanine and be able to show better controlled release behavior. The stability and controlled release nature of RADA-F6 in different conditions followed by 5-FU entrapment at in silico conditions was confirmed by molecular dynamics simulation taking RADA-16 as control. Cytotoxicity of the drug-loaded RADA-F6 was measured by MTT assay and cellular uptake by confocal microscopy. Physicochemical characterization and further Western blot analysis and flow cytometric studies confirm that RADA-F6 can be successfully used as an efficient vector for pH-sensitive, controlled 5-FU delivery system. Keywords: scaffold, drug delivery, nanofibrous, aromaticAshwanikumar NKumar NASaneesh Babu PSSivakumar KCVadakkan MVNair PHema Saranya IAsha Nair SVinod Kumar GSDove Medical Pressarticlescaffolddrug deliverynanofibrousaromaticMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5583-5594 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	scaffold drug delivery nanofibrous aromatic Medicine (General) R5-920
spellingShingle	scaffold drug delivery nanofibrous aromatic Medicine (General) R5-920 Ashwanikumar N Kumar NA Saneesh Babu PS Sivakumar KC Vadakkan MV Nair P Hema Saranya I Asha Nair S Vinod Kumar GS Self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil
description	Narayanan Ashwanikumar,1 Nisha Asok Kumar,2 Padma S Saneesh Babu,2 Krishnankutty C Sivakumar,3 Mithun Varghese Vadakkan,1 Parvathi Nair,1 Ilamathi Hema Saranya,1 Sivakumari Asha Nair,2 Gopalakrishnapillai S Vinod Kumar1 1Chemical Biology, Nano Drug Delivery Systems, Bio-Innovation Center, 2Cancer Research Programme, 3Distributed Information Sub-Centre (Bioinformatics Centre), Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India Abstract: The study shows that RADA-F6 peptide with pH-responsive self-assembling nature can be effectively used as a drug delivery system for the sustained release of a potent anticancer drug 5-fluorouracil (5-FU) at basic pH. As 5-FU contains the aromatic pyrimidine ring, RADA-F6 system is suitable for entrapping an aromatic drug due to effective π–π stacking with phenylalanine and be able to show better controlled release behavior. The stability and controlled release nature of RADA-F6 in different conditions followed by 5-FU entrapment at in silico conditions was confirmed by molecular dynamics simulation taking RADA-16 as control. Cytotoxicity of the drug-loaded RADA-F6 was measured by MTT assay and cellular uptake by confocal microscopy. Physicochemical characterization and further Western blot analysis and flow cytometric studies confirm that RADA-F6 can be successfully used as an efficient vector for pH-sensitive, controlled 5-FU delivery system. Keywords: scaffold, drug delivery, nanofibrous, aromatic
format	article
author	Ashwanikumar N Kumar NA Saneesh Babu PS Sivakumar KC Vadakkan MV Nair P Hema Saranya I Asha Nair S Vinod Kumar GS
author_facet	Ashwanikumar N Kumar NA Saneesh Babu PS Sivakumar KC Vadakkan MV Nair P Hema Saranya I Asha Nair S Vinod Kumar GS
author_sort	Ashwanikumar N
title	Self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil
title_short	Self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil
title_full	Self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil
title_fullStr	Self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil
title_full_unstemmed	Self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil
title_sort	self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/3db97da7dc4042c68371541f1c3b655e
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Self-assembling peptide nanofibers containing phenylalanine for the controlled release of 5-fluorouracil

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