Role of Ras in regulation of intestinal epithelial cell homeostasis and crosstalk with Wnt signaling.

Cross talk between different signaling pathways is thought to be important for regulation of homeostasis of, as well as oncogenesis of, the intestinal epithelium. Expression of an active form of K-Ras specifically in intestinal epithelial cells (IECs) of mice (IEC-RasDA mice) resulted in the develop...

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Autores principales:	Takenori Kotani, Noriko Ihara, Saki Okamoto, Jajar Setiawan, Tasuku Konno, Yasuyuki Saito, Yoji Murata, Takashi Matozaki
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3dc6677168d443738bc23e40aae51d3f
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spelling	oai:doaj.org-article:3dc6677168d443738bc23e40aae51d3f2021-12-02T20:19:27ZRole of Ras in regulation of intestinal epithelial cell homeostasis and crosstalk with Wnt signaling.1932-620310.1371/journal.pone.0256774https://doaj.org/article/3dc6677168d443738bc23e40aae51d3f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256774https://doaj.org/toc/1932-6203Cross talk between different signaling pathways is thought to be important for regulation of homeostasis of, as well as oncogenesis of, the intestinal epithelium. Expression of an active form of K-Ras specifically in intestinal epithelial cells (IECs) of mice (IEC-RasDA mice) resulted in the development of hyperplasia in the small intestine and colon of mice. IEC-RasDA mice also manifested the increased proliferation of IECs. In addition, the number of goblet cells markedly increased, while that of Paneth cells decreased in IEC-RasDA mice. Development of intestinal organoids was markedly enhanced for IEC-RasDA mice compared with control mice. Whereas, the expression of Wnt target genes was significantly reduced in the in intestinal crypts from IEC-RasDA mice compared with that apparent for the control. Our results thus suggest that K-Ras promotes the proliferation of IECs as well as generation of goblet cells. By contrast, Ras counter-regulates the Wnt signaling and thereby contribute to the proper regulation of intestinal epithelial cell homeostasis.Takenori KotaniNoriko IharaSaki OkamotoJajar SetiawanTasuku KonnoYasuyuki SaitoYoji MurataTakashi MatozakiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256774 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takenori Kotani Noriko Ihara Saki Okamoto Jajar Setiawan Tasuku Konno Yasuyuki Saito Yoji Murata Takashi Matozaki Role of Ras in regulation of intestinal epithelial cell homeostasis and crosstalk with Wnt signaling.
description	Cross talk between different signaling pathways is thought to be important for regulation of homeostasis of, as well as oncogenesis of, the intestinal epithelium. Expression of an active form of K-Ras specifically in intestinal epithelial cells (IECs) of mice (IEC-RasDA mice) resulted in the development of hyperplasia in the small intestine and colon of mice. IEC-RasDA mice also manifested the increased proliferation of IECs. In addition, the number of goblet cells markedly increased, while that of Paneth cells decreased in IEC-RasDA mice. Development of intestinal organoids was markedly enhanced for IEC-RasDA mice compared with control mice. Whereas, the expression of Wnt target genes was significantly reduced in the in intestinal crypts from IEC-RasDA mice compared with that apparent for the control. Our results thus suggest that K-Ras promotes the proliferation of IECs as well as generation of goblet cells. By contrast, Ras counter-regulates the Wnt signaling and thereby contribute to the proper regulation of intestinal epithelial cell homeostasis.
format	article
author	Takenori Kotani Noriko Ihara Saki Okamoto Jajar Setiawan Tasuku Konno Yasuyuki Saito Yoji Murata Takashi Matozaki
author_facet	Takenori Kotani Noriko Ihara Saki Okamoto Jajar Setiawan Tasuku Konno Yasuyuki Saito Yoji Murata Takashi Matozaki
author_sort	Takenori Kotani
title	Role of Ras in regulation of intestinal epithelial cell homeostasis and crosstalk with Wnt signaling.
title_short	Role of Ras in regulation of intestinal epithelial cell homeostasis and crosstalk with Wnt signaling.
title_full	Role of Ras in regulation of intestinal epithelial cell homeostasis and crosstalk with Wnt signaling.
title_fullStr	Role of Ras in regulation of intestinal epithelial cell homeostasis and crosstalk with Wnt signaling.
title_full_unstemmed	Role of Ras in regulation of intestinal epithelial cell homeostasis and crosstalk with Wnt signaling.
title_sort	role of ras in regulation of intestinal epithelial cell homeostasis and crosstalk with wnt signaling.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3dc6677168d443738bc23e40aae51d3f
work_keys_str_mv	AT takenorikotani roleofrasinregulationofintestinalepithelialcellhomeostasisandcrosstalkwithwntsignaling AT norikoihara roleofrasinregulationofintestinalepithelialcellhomeostasisandcrosstalkwithwntsignaling AT sakiokamoto roleofrasinregulationofintestinalepithelialcellhomeostasisandcrosstalkwithwntsignaling AT jajarsetiawan roleofrasinregulationofintestinalepithelialcellhomeostasisandcrosstalkwithwntsignaling AT tasukukonno roleofrasinregulationofintestinalepithelialcellhomeostasisandcrosstalkwithwntsignaling AT yasuyukisaito roleofrasinregulationofintestinalepithelialcellhomeostasisandcrosstalkwithwntsignaling AT yojimurata roleofrasinregulationofintestinalepithelialcellhomeostasisandcrosstalkwithwntsignaling AT takashimatozaki roleofrasinregulationofintestinalepithelialcellhomeostasisandcrosstalkwithwntsignaling
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Role of Ras in regulation of intestinal epithelial cell homeostasis and crosstalk with Wnt signaling.

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