Short Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq.

Abstract. Faramayuda F, Mariani TS, Elfahmi, Sukrasno. 2020. Short Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq. Biodiversitas 21: 4967-4972. Orthosiphon aristatus (Blume) Miq. are known to have many benefits, including stimulating urine e...

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Autores principales:	Fahrauk Faramayuda, TOTIK SRI MARIANI, ELFAHMI ELFAHMI, SUKRASNO SUKRASNO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	2,4-d, growth regulatory, orthosiphon aristatus, plant tissue culture, secondary metabolites identification, variety Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3dc81a0349964e74b81a4071933526ca
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spelling	oai:doaj.org-article:3dc81a0349964e74b81a4071933526ca2021-11-22T00:51:08ZShort Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq.1412-033X2085-472210.13057/biodiv/d211063https://doaj.org/article/3dc81a0349964e74b81a4071933526ca2020-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6569https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Faramayuda F, Mariani TS, Elfahmi, Sukrasno. 2020. Short Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq. Biodiversitas 21: 4967-4972. Orthosiphon aristatus (Blume) Miq. are known to have many benefits, including stimulating urine expenditure (diuretics) and dissolving kidney stones. O. aristatus widely planted in Indonesia are purple and white-purple. The main secondary metabolite components of O. aristatus are sinensetin, rosmarinic acid, and eupatorin. One of the initial steps to increase secondary metabolites in O. aristatus is by induction of callus using plant tissue, which later can be developed into a culture suspension for secondary metabolites. The materials used are the leaf of two varieties of O. aristatus that have been sterilized and grown on Murashige and Skoog media with growth regulatory 2,4-dichlorophenoxyacetic acid at a concentration of 0.4;1.0; 2.0 mg/L. The identification of secondary metabolites of callus was carried out by thin-layer chromatography. The best growth regulating agent for callus induction on the leaves of purple and white-purple varieties of O. aristatus is 2,4-D 0.4 mg/L on Murashige and Skoog media. These media can grow callus at a faster time, friable, and slightly white-yellow color. The identification of secondary metabolites in callus acetone extract showed the presence of sinensetin and rosmarinic acid.Fahrauk FaramayudaTOTIK SRI MARIANIELFAHMI ELFAHMISUKRASNO SUKRASNOMBI & UNS Soloarticle2,4-d, growth regulatory, orthosiphon aristatus, plant tissue culture, secondary metabolites identification, varietyBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	2,4-d, growth regulatory, orthosiphon aristatus, plant tissue culture, secondary metabolites identification, variety Biology (General) QH301-705.5
spellingShingle	2,4-d, growth regulatory, orthosiphon aristatus, plant tissue culture, secondary metabolites identification, variety Biology (General) QH301-705.5 Fahrauk Faramayuda TOTIK SRI MARIANI ELFAHMI ELFAHMI SUKRASNO SUKRASNO Short Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq.
description	Abstract. Faramayuda F, Mariani TS, Elfahmi, Sukrasno. 2020. Short Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq. Biodiversitas 21: 4967-4972. Orthosiphon aristatus (Blume) Miq. are known to have many benefits, including stimulating urine expenditure (diuretics) and dissolving kidney stones. O. aristatus widely planted in Indonesia are purple and white-purple. The main secondary metabolite components of O. aristatus are sinensetin, rosmarinic acid, and eupatorin. One of the initial steps to increase secondary metabolites in O. aristatus is by induction of callus using plant tissue, which later can be developed into a culture suspension for secondary metabolites. The materials used are the leaf of two varieties of O. aristatus that have been sterilized and grown on Murashige and Skoog media with growth regulatory 2,4-dichlorophenoxyacetic acid at a concentration of 0.4;1.0; 2.0 mg/L. The identification of secondary metabolites of callus was carried out by thin-layer chromatography. The best growth regulating agent for callus induction on the leaves of purple and white-purple varieties of O. aristatus is 2,4-D 0.4 mg/L on Murashige and Skoog media. These media can grow callus at a faster time, friable, and slightly white-yellow color. The identification of secondary metabolites in callus acetone extract showed the presence of sinensetin and rosmarinic acid.
format	article
author	Fahrauk Faramayuda TOTIK SRI MARIANI ELFAHMI ELFAHMI SUKRASNO SUKRASNO
author_facet	Fahrauk Faramayuda TOTIK SRI MARIANI ELFAHMI ELFAHMI SUKRASNO SUKRASNO
author_sort	Fahrauk Faramayuda
title	Short Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq.
title_short	Short Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq.
title_full	Short Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq.
title_fullStr	Short Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq.
title_full_unstemmed	Short Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq.
title_sort	short communication: callus induction in purple and white-purple varieties of orthosiphon aristatus (blume) miq.
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/3dc81a0349964e74b81a4071933526ca
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Short Communication: Callus induction in purple and white-purple varieties of Orthosiphon aristatus (Blume) Miq.

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