A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock

With an increased consumption of seafood products, food fraud with fish resources has been continuously reported. In particular, codfish has been exploited worldwide as a processed product in fresh, frozen, smoked, canned, or ready-to-eat dish forms. However, it is challenging to identify processed...

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Autores principales:	Yu-Min Lee, Shinyoung Lee, Hae-Yeong Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cod multiplex PCR species identification capillary electrophoresis Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/3dc86a390f5a4fb0a8a2dde21652f7ab
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spelling	oai:doaj.org-article:3dc86a390f5a4fb0a8a2dde21652f7ab2021-11-25T17:33:38ZA Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock10.3390/foods101126312304-8158https://doaj.org/article/3dc86a390f5a4fb0a8a2dde21652f7ab2021-10-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2631https://doaj.org/toc/2304-8158With an increased consumption of seafood products, food fraud with fish resources has been continuously reported. In particular, codfish has been exploited worldwide as a processed product in fresh, frozen, smoked, canned, or ready-to-eat dish forms. However, it is challenging to identify processed fish products after processing because of their similar morphological characteristics. Substitution and mislabeling of codfish among different species are also happening deliberately or unintentionally. Thus, it is necessary to distinguish cod species to prevent fish adulteration and food fraud. In this study, we developed a multiplex PCR for simultaneously identifying five cod species within <i>Gadidae</i> using capillary electrophoresis. Then, their species-specific primer sets were designed by targeting the mitochondrial <i>cytochrome b</i> gene. Subsequently, the amplicon sizes obtained were 237 bp, 204 bp, 164 bp, 138 bp, and 98 bp for Atlantic cod, Pacific cod, blue whiting, haddock, and Alaska pollock, respectively. The specificity of each primer was further tested using 19 fish species, and no cross-reactivity was observed. The limit of detection of this multiplex PCR assay was 1 pg. The developed multiplex PCR assay can be applied to 40 commercial food products successfully. This detection method will be efficient for managing seafood authentication by simultaneously analyzing multiple cod species.Yu-Min LeeShinyoung LeeHae-Yeong KimMDPI AGarticlecodmultiplex PCRspecies identificationcapillary electrophoresisChemical technologyTP1-1185ENFoods, Vol 10, Iss 2631, p 2631 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cod multiplex PCR species identification capillary electrophoresis Chemical technology TP1-1185
spellingShingle	cod multiplex PCR species identification capillary electrophoresis Chemical technology TP1-1185 Yu-Min Lee Shinyoung Lee Hae-Yeong Kim A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock
description	With an increased consumption of seafood products, food fraud with fish resources has been continuously reported. In particular, codfish has been exploited worldwide as a processed product in fresh, frozen, smoked, canned, or ready-to-eat dish forms. However, it is challenging to identify processed fish products after processing because of their similar morphological characteristics. Substitution and mislabeling of codfish among different species are also happening deliberately or unintentionally. Thus, it is necessary to distinguish cod species to prevent fish adulteration and food fraud. In this study, we developed a multiplex PCR for simultaneously identifying five cod species within <i>Gadidae</i> using capillary electrophoresis. Then, their species-specific primer sets were designed by targeting the mitochondrial <i>cytochrome b</i> gene. Subsequently, the amplicon sizes obtained were 237 bp, 204 bp, 164 bp, 138 bp, and 98 bp for Atlantic cod, Pacific cod, blue whiting, haddock, and Alaska pollock, respectively. The specificity of each primer was further tested using 19 fish species, and no cross-reactivity was observed. The limit of detection of this multiplex PCR assay was 1 pg. The developed multiplex PCR assay can be applied to 40 commercial food products successfully. This detection method will be efficient for managing seafood authentication by simultaneously analyzing multiple cod species.
format	article
author	Yu-Min Lee Shinyoung Lee Hae-Yeong Kim
author_facet	Yu-Min Lee Shinyoung Lee Hae-Yeong Kim
author_sort	Yu-Min Lee
title	A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock
title_short	A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock
title_full	A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock
title_fullStr	A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock
title_full_unstemmed	A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock
title_sort	multiplex pcr assay combined with capillary electrophoresis for the simultaneous identification of atlantic cod, pacific cod, blue whiting, haddock, and alaska pollock
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3dc86a390f5a4fb0a8a2dde21652f7ab
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A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock

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