Rapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice

Abstract We developed a non-destructive and rapid whole-mount bone staining method for small fish, Xenopus laevis, and rodent fetuses (RAP-B). RAP-B does not require skin or soft tissue removal. However, RAP-B requires hair removal from hairy animals, such as adult mice and rats. In the present stud...

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Autores principales:	Nobuo Kariyama, Hiromi Sakata-Haga, Tsuyoshi Tsukada, Hiroki Shimada, Makoto Taniguchi, Toshihisa Hatta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3dcd85bd2426465ab83ae02b7881c81a
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spelling	oai:doaj.org-article:3dcd85bd2426465ab83ae02b7881c81a2021-12-02T15:23:28ZRapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice10.1038/s41598-021-81616-72045-2322https://doaj.org/article/3dcd85bd2426465ab83ae02b7881c81a2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81616-7https://doaj.org/toc/2045-2322Abstract We developed a non-destructive and rapid whole-mount bone staining method for small fish, Xenopus laevis, and rodent fetuses (RAP-B). RAP-B does not require skin or soft tissue removal. However, RAP-B requires hair removal from hairy animals, such as adult mice and rats. In the present study, we investigated hair removal chemical treatments that did not result in soft tissue destruction. The hair removal effectiveness was investigated using a calcium mercaptoacetate or sodium mercaptoacetate solution on skin fragments obtained from the back of adult mice. A mixture of 2% sodium mercaptoacetate in 3% potassium hydroxide was found to be the most effective in complete hair removal from the skin. Using this hair removal treatment as a pretreatment for RAP-B, the preparation of fast-acting artifact-free whole-mount bone staining was possible without skin and soft tissue removal (RAP-B/HR). We performed a seamless observation from a low magnification wide-view to a high magnification without artifactacting artifacts using fluorescence zoom microscopy. Therefore, the combination of RAP-B/HR and fluorescent zoom microscopy is a novel platform for three-dimensional, wide-field, high-resolution pathological anatomical analysis.Nobuo KariyamaHiromi Sakata-HagaTsuyoshi TsukadaHiroki ShimadaMakoto TaniguchiToshihisa HattaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nobuo Kariyama Hiromi Sakata-Haga Tsuyoshi Tsukada Hiroki Shimada Makoto Taniguchi Toshihisa Hatta Rapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice
description	Abstract We developed a non-destructive and rapid whole-mount bone staining method for small fish, Xenopus laevis, and rodent fetuses (RAP-B). RAP-B does not require skin or soft tissue removal. However, RAP-B requires hair removal from hairy animals, such as adult mice and rats. In the present study, we investigated hair removal chemical treatments that did not result in soft tissue destruction. The hair removal effectiveness was investigated using a calcium mercaptoacetate or sodium mercaptoacetate solution on skin fragments obtained from the back of adult mice. A mixture of 2% sodium mercaptoacetate in 3% potassium hydroxide was found to be the most effective in complete hair removal from the skin. Using this hair removal treatment as a pretreatment for RAP-B, the preparation of fast-acting artifact-free whole-mount bone staining was possible without skin and soft tissue removal (RAP-B/HR). We performed a seamless observation from a low magnification wide-view to a high magnification without artifactacting artifacts using fluorescence zoom microscopy. Therefore, the combination of RAP-B/HR and fluorescent zoom microscopy is a novel platform for three-dimensional, wide-field, high-resolution pathological anatomical analysis.
format	article
author	Nobuo Kariyama Hiromi Sakata-Haga Tsuyoshi Tsukada Hiroki Shimada Makoto Taniguchi Toshihisa Hatta
author_facet	Nobuo Kariyama Hiromi Sakata-Haga Tsuyoshi Tsukada Hiroki Shimada Makoto Taniguchi Toshihisa Hatta
author_sort	Nobuo Kariyama
title	Rapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice
title_short	Rapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice
title_full	Rapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice
title_fullStr	Rapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice
title_full_unstemmed	Rapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice
title_sort	rapid bone staining with hair removal (rap-b/hr): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3dcd85bd2426465ab83ae02b7881c81a
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Rapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice

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