Characterization of a Multidrug-Resistant Porcine <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Sequence Type 11 Strain Coharboring <italic toggle="yes">bla</italic><sub>KPC-2</sub> and <italic toggle="yes">fosA3</italic> on Two Novel Hybrid Plasmids

ABSTRACT The occurrence of carbapenemase-producing Enterobacteriaceae (CPE) poses a considerable risk for public health. The gene for Klebsiella pneumoniae carbapenemase-2 (KPC-2) has been reported in many countries worldwide, and KPC-2-producing strains are mainly of human origin. In this study, we...

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Autores principales:	Wanjiang Zhang, Yao Zhu, Changzhen Wang, Wenyu Liu, Ruichao Li, Fuguang Chen, Tian Luan, Yanhe Zhang, Stefan Schwarz, Siguo Liu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	KPC-2 carbapenem resistance plasmid food-producing animal Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3dcf7825556b4d7e8a2047cd3ab1851f
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spelling	oai:doaj.org-article:3dcf7825556b4d7e8a2047cd3ab1851f2021-11-15T15:27:32ZCharacterization of a Multidrug-Resistant Porcine <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Sequence Type 11 Strain Coharboring <italic toggle="yes">bla</italic><sub>KPC-2</sub> and <italic toggle="yes">fosA3</italic> on Two Novel Hybrid Plasmids10.1128/mSphere.00590-192379-5042https://doaj.org/article/3dcf7825556b4d7e8a2047cd3ab1851f2019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00590-19https://doaj.org/toc/2379-5042ABSTRACT The occurrence of carbapenemase-producing Enterobacteriaceae (CPE) poses a considerable risk for public health. The gene for Klebsiella pneumoniae carbapenemase-2 (KPC-2) has been reported in many countries worldwide, and KPC-2-producing strains are mainly of human origin. In this study, we identified two novel hybrid plasmids that carry either blaKPC-2 or the fosfomycin resistance gene fosA3 in the multiresistant K. pneumoniae isolate K15 of swine origin in China. The blaKPC-2-bearing plasmid pK15-KPC was a fusion derivative of an IncF33:A−:B− incompatibility group (Inc) plasmid and chromosomal sequences of K. pneumoniae (CSKP). A 5-bp direct target sequence duplication (GACTA) was identified at the boundaries of the CSKP, suggesting that the integration might have been due to a transposition event. The blaKPC-2 gene on pK15-KPC was in a derivative of ΔTn6296-1. The multireplicon fosA3-carrying IncN-IncR plasmid pK15-FOS also showed a mosaic structure, possibly originating from a recombination between an epidemic fosA3-carrying pHN7A8-like plasmid and a pKPC-LK30-like IncR plasmid. Stability tests demonstrated that both novel hybrid plasmids were stably maintained in the original host without antibiotic selection but were lost from the transformants after approximately 200 generations. This is apparently the first description of a porcine sequence type 11 (ST11) K. pneumoniae isolate coproducing KPC-2 and FosA3 via pK15-KPC and pK15-FOS, respectively. The multidrug resistance (MDR) phenotype of this high-risk K. pneumoniae isolate may contribute to its spread and its persistence. IMPORTANCE The global dissemination of carbapenem resistance genes is of great concern. Animals are usually considered a reservoir of resistance genes and an important source of human infection. Although carbapenemase-producing Enterobacteriaceae strains of animal origin have been reported increasingly, blaKPC-2-positive strains from food-producing animals are still rare. In this study, we first describe the isolation and characterization of a carbapenem-resistant Klebsiella pneumoniae ST11 isolate, strain K15, which is of pig origin and coproduces KPC-2 and FosA3 via two novel hybrid plasmids. Furthermore, our findings highlight that this ST11 Klebsiella pneumoniae strain K15 is most likely of human origin and could be easily transmitted back to humans via direct contact or food intake. In light of our findings, significant attention must be paid to monitoring the prevalence and further evolution of blaKPC-2-carrying plasmids among the Enterobacteriaceae strains of animal origin.Wanjiang ZhangYao ZhuChangzhen WangWenyu LiuRuichao LiFuguang ChenTian LuanYanhe ZhangStefan SchwarzSiguo LiuAmerican Society for MicrobiologyarticleKPC-2carbapenem resistanceplasmidfood-producing animalMicrobiologyQR1-502ENmSphere, Vol 4, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	KPC-2 carbapenem resistance plasmid food-producing animal Microbiology QR1-502
spellingShingle	KPC-2 carbapenem resistance plasmid food-producing animal Microbiology QR1-502 Wanjiang Zhang Yao Zhu Changzhen Wang Wenyu Liu Ruichao Li Fuguang Chen Tian Luan Yanhe Zhang Stefan Schwarz Siguo Liu Characterization of a Multidrug-Resistant Porcine <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Sequence Type 11 Strain Coharboring <italic toggle="yes">bla</italic><sub>KPC-2</sub> and <italic toggle="yes">fosA3</italic> on Two Novel Hybrid Plasmids
description	ABSTRACT The occurrence of carbapenemase-producing Enterobacteriaceae (CPE) poses a considerable risk for public health. The gene for Klebsiella pneumoniae carbapenemase-2 (KPC-2) has been reported in many countries worldwide, and KPC-2-producing strains are mainly of human origin. In this study, we identified two novel hybrid plasmids that carry either blaKPC-2 or the fosfomycin resistance gene fosA3 in the multiresistant K. pneumoniae isolate K15 of swine origin in China. The blaKPC-2-bearing plasmid pK15-KPC was a fusion derivative of an IncF33:A−:B− incompatibility group (Inc) plasmid and chromosomal sequences of K. pneumoniae (CSKP). A 5-bp direct target sequence duplication (GACTA) was identified at the boundaries of the CSKP, suggesting that the integration might have been due to a transposition event. The blaKPC-2 gene on pK15-KPC was in a derivative of ΔTn6296-1. The multireplicon fosA3-carrying IncN-IncR plasmid pK15-FOS also showed a mosaic structure, possibly originating from a recombination between an epidemic fosA3-carrying pHN7A8-like plasmid and a pKPC-LK30-like IncR plasmid. Stability tests demonstrated that both novel hybrid plasmids were stably maintained in the original host without antibiotic selection but were lost from the transformants after approximately 200 generations. This is apparently the first description of a porcine sequence type 11 (ST11) K. pneumoniae isolate coproducing KPC-2 and FosA3 via pK15-KPC and pK15-FOS, respectively. The multidrug resistance (MDR) phenotype of this high-risk K. pneumoniae isolate may contribute to its spread and its persistence. IMPORTANCE The global dissemination of carbapenem resistance genes is of great concern. Animals are usually considered a reservoir of resistance genes and an important source of human infection. Although carbapenemase-producing Enterobacteriaceae strains of animal origin have been reported increasingly, blaKPC-2-positive strains from food-producing animals are still rare. In this study, we first describe the isolation and characterization of a carbapenem-resistant Klebsiella pneumoniae ST11 isolate, strain K15, which is of pig origin and coproduces KPC-2 and FosA3 via two novel hybrid plasmids. Furthermore, our findings highlight that this ST11 Klebsiella pneumoniae strain K15 is most likely of human origin and could be easily transmitted back to humans via direct contact or food intake. In light of our findings, significant attention must be paid to monitoring the prevalence and further evolution of blaKPC-2-carrying plasmids among the Enterobacteriaceae strains of animal origin.
format	article
author	Wanjiang Zhang Yao Zhu Changzhen Wang Wenyu Liu Ruichao Li Fuguang Chen Tian Luan Yanhe Zhang Stefan Schwarz Siguo Liu
author_facet	Wanjiang Zhang Yao Zhu Changzhen Wang Wenyu Liu Ruichao Li Fuguang Chen Tian Luan Yanhe Zhang Stefan Schwarz Siguo Liu
author_sort	Wanjiang Zhang
title	Characterization of a Multidrug-Resistant Porcine <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Sequence Type 11 Strain Coharboring <italic toggle="yes">bla</italic><sub>KPC-2</sub> and <italic toggle="yes">fosA3</italic> on Two Novel Hybrid Plasmids
title_short	Characterization of a Multidrug-Resistant Porcine <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Sequence Type 11 Strain Coharboring <italic toggle="yes">bla</italic><sub>KPC-2</sub> and <italic toggle="yes">fosA3</italic> on Two Novel Hybrid Plasmids
title_full	Characterization of a Multidrug-Resistant Porcine <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Sequence Type 11 Strain Coharboring <italic toggle="yes">bla</italic><sub>KPC-2</sub> and <italic toggle="yes">fosA3</italic> on Two Novel Hybrid Plasmids
title_fullStr	Characterization of a Multidrug-Resistant Porcine <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Sequence Type 11 Strain Coharboring <italic toggle="yes">bla</italic><sub>KPC-2</sub> and <italic toggle="yes">fosA3</italic> on Two Novel Hybrid Plasmids
title_full_unstemmed	Characterization of a Multidrug-Resistant Porcine <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Sequence Type 11 Strain Coharboring <italic toggle="yes">bla</italic><sub>KPC-2</sub> and <italic toggle="yes">fosA3</italic> on Two Novel Hybrid Plasmids
title_sort	characterization of a multidrug-resistant porcine <named-content content-type="genus-species">klebsiella pneumoniae</named-content> sequence type 11 strain coharboring <italic toggle="yes">bla</italic><sub>kpc-2</sub> and <italic toggle="yes">fosa3</italic> on two novel hybrid plasmids
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/3dcf7825556b4d7e8a2047cd3ab1851f
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Characterization of a Multidrug-Resistant Porcine <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Sequence Type 11 Strain Coharboring <italic toggle="yes">bla</italic><sub>KPC-2</sub> and <italic toggle="yes">fosA3</italic> on Two Novel Hybrid Plasmids

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