Mechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa
Abstract Hp1404, identified from the venom of the scorpion Heterometrus petersii, displays antimicrobial activity with cytotoxicity. Several synthetic peptides were designed based on the parent peptide Hp1404 to reduce cytotoxicity and improve activity (deletion of glycine and phenylalanine, substit...
Guardado en:
Autores principales: | , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2018
|
Materias: | |
Acceso en línea: | https://doaj.org/article/3dcffcc10a094df7ae0b517f7a4d43bc |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:3dcffcc10a094df7ae0b517f7a4d43bc |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:3dcffcc10a094df7ae0b517f7a4d43bc2021-12-02T15:09:12ZMechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa10.1038/s41598-018-19434-72045-2322https://doaj.org/article/3dcffcc10a094df7ae0b517f7a4d43bc2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19434-7https://doaj.org/toc/2045-2322Abstract Hp1404, identified from the venom of the scorpion Heterometrus petersii, displays antimicrobial activity with cytotoxicity. Several synthetic peptides were designed based on the parent peptide Hp1404 to reduce cytotoxicity and improve activity (deletion of glycine and phenylalanine, substitution with leucine and lysine). The analogue peptides generated comprised 12 amino acids and displayed amphipathic α-helical structures, with higher hydrophobic moments and net positive charge than those of the Hp1404. The analogues showed less hemolytic and toxic effects toward mammalian cells than the Hp1404, especially Hp1404-T1e, which exhibited particularly potent antibacterial and antibiofilm activities against multidrug-resistant Pseudomonas aeruginosa (MRPA) strains. The analogue peptide Hp1404-T1e was more stable against salt and trypsin than the Hp1404. Hp1404’s mechanism of action involves binding to lipopolysaccharide (LPS), thereby killing bacteria through membrane disruption. Hp1404-T1e kills bacteria more rapidly than Hp1404 and not only seems to bind more strongly to LPS but may also be able to enter bacterial cells and interact with their DNA. Additionally, Hp1404-T1e can effectively kill bacteria in vivo. The results of this study indicate that Hp1404-T1e not only displays antimicrobial activity, but is also functional in physiological conditions, confirming its potential use as an effective therapeutic agent against MRPA.Min Kyung KimHee Kyoung KangSu Jin KoMin Ji HongJeong Kyu BangChang Ho SeoYoonkyung ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-16 (2018) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Min Kyung Kim Hee Kyoung Kang Su Jin Ko Min Ji Hong Jeong Kyu Bang Chang Ho Seo Yoonkyung Park Mechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa |
description |
Abstract Hp1404, identified from the venom of the scorpion Heterometrus petersii, displays antimicrobial activity with cytotoxicity. Several synthetic peptides were designed based on the parent peptide Hp1404 to reduce cytotoxicity and improve activity (deletion of glycine and phenylalanine, substitution with leucine and lysine). The analogue peptides generated comprised 12 amino acids and displayed amphipathic α-helical structures, with higher hydrophobic moments and net positive charge than those of the Hp1404. The analogues showed less hemolytic and toxic effects toward mammalian cells than the Hp1404, especially Hp1404-T1e, which exhibited particularly potent antibacterial and antibiofilm activities against multidrug-resistant Pseudomonas aeruginosa (MRPA) strains. The analogue peptide Hp1404-T1e was more stable against salt and trypsin than the Hp1404. Hp1404’s mechanism of action involves binding to lipopolysaccharide (LPS), thereby killing bacteria through membrane disruption. Hp1404-T1e kills bacteria more rapidly than Hp1404 and not only seems to bind more strongly to LPS but may also be able to enter bacterial cells and interact with their DNA. Additionally, Hp1404-T1e can effectively kill bacteria in vivo. The results of this study indicate that Hp1404-T1e not only displays antimicrobial activity, but is also functional in physiological conditions, confirming its potential use as an effective therapeutic agent against MRPA. |
format |
article |
author |
Min Kyung Kim Hee Kyoung Kang Su Jin Ko Min Ji Hong Jeong Kyu Bang Chang Ho Seo Yoonkyung Park |
author_facet |
Min Kyung Kim Hee Kyoung Kang Su Jin Ko Min Ji Hong Jeong Kyu Bang Chang Ho Seo Yoonkyung Park |
author_sort |
Min Kyung Kim |
title |
Mechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa |
title_short |
Mechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa |
title_full |
Mechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa |
title_fullStr |
Mechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa |
title_full_unstemmed |
Mechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa |
title_sort |
mechanisms driving the antibacterial and antibiofilm properties of hp1404 and its analogue peptides against multidrug-resistant pseudomonas aeruginosa |
publisher |
Nature Portfolio |
publishDate |
2018 |
url |
https://doaj.org/article/3dcffcc10a094df7ae0b517f7a4d43bc |
work_keys_str_mv |
AT minkyungkim mechanismsdrivingtheantibacterialandantibiofilmpropertiesofhp1404anditsanaloguepeptidesagainstmultidrugresistantpseudomonasaeruginosa AT heekyoungkang mechanismsdrivingtheantibacterialandantibiofilmpropertiesofhp1404anditsanaloguepeptidesagainstmultidrugresistantpseudomonasaeruginosa AT sujinko mechanismsdrivingtheantibacterialandantibiofilmpropertiesofhp1404anditsanaloguepeptidesagainstmultidrugresistantpseudomonasaeruginosa AT minjihong mechanismsdrivingtheantibacterialandantibiofilmpropertiesofhp1404anditsanaloguepeptidesagainstmultidrugresistantpseudomonasaeruginosa AT jeongkyubang mechanismsdrivingtheantibacterialandantibiofilmpropertiesofhp1404anditsanaloguepeptidesagainstmultidrugresistantpseudomonasaeruginosa AT changhoseo mechanismsdrivingtheantibacterialandantibiofilmpropertiesofhp1404anditsanaloguepeptidesagainstmultidrugresistantpseudomonasaeruginosa AT yoonkyungpark mechanismsdrivingtheantibacterialandantibiofilmpropertiesofhp1404anditsanaloguepeptidesagainstmultidrugresistantpseudomonasaeruginosa |
_version_ |
1718387868198502400 |