Comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program.
Understanding how the limb blastema is established after the initial wound healing response is an important aspect of regeneration research. Here we performed parallel expression profile time courses of healing lateral wounds versus amputated limbs in axolotl. This comparison between wound healing a...
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oai:doaj.org-article:3dd2bd81bd9449bb9839b6659253921b2021-11-18T07:47:09ZComparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program.1932-620310.1371/journal.pone.0061352https://doaj.org/article/3dd2bd81bd9449bb9839b6659253921b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23658691/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Understanding how the limb blastema is established after the initial wound healing response is an important aspect of regeneration research. Here we performed parallel expression profile time courses of healing lateral wounds versus amputated limbs in axolotl. This comparison between wound healing and regeneration allowed us to identify amputation-specific genes. By clustering the expression profiles of these samples, we could detect three distinguishable phases of gene expression - early wound healing followed by a transition-phase leading to establishment of the limb development program, which correspond to the three phases of limb regeneration that had been defined by morphological criteria. By focusing on the transition-phase, we identified 93 strictly amputation-associated genes many of which are implicated in oxidative-stress response, chromatin modification, epithelial development or limb development. We further classified the genes based on whether they were or were not significantly expressed in the developing limb bud. The specific localization of 53 selected candidates within the blastema was investigated by in situ hybridization. In summary, we identified a set of genes that are expressed specifically during regeneration and are therefore, likely candidates for the regulation of blastema formation.Dunja KnappHerbert SchulzCynthia Alexander RasconMichael VolkmerJuliane ScholzEugen NacuMu LeSergey NovozhilovAkira TazakiStephanie ProtzeTina JacobNorbert HubnerBianca HabermannElly M TanakaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e61352 (2013) |
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Medicine R Science Q |
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Medicine R Science Q Dunja Knapp Herbert Schulz Cynthia Alexander Rascon Michael Volkmer Juliane Scholz Eugen Nacu Mu Le Sergey Novozhilov Akira Tazaki Stephanie Protze Tina Jacob Norbert Hubner Bianca Habermann Elly M Tanaka Comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program. |
| description |
Understanding how the limb blastema is established after the initial wound healing response is an important aspect of regeneration research. Here we performed parallel expression profile time courses of healing lateral wounds versus amputated limbs in axolotl. This comparison between wound healing and regeneration allowed us to identify amputation-specific genes. By clustering the expression profiles of these samples, we could detect three distinguishable phases of gene expression - early wound healing followed by a transition-phase leading to establishment of the limb development program, which correspond to the three phases of limb regeneration that had been defined by morphological criteria. By focusing on the transition-phase, we identified 93 strictly amputation-associated genes many of which are implicated in oxidative-stress response, chromatin modification, epithelial development or limb development. We further classified the genes based on whether they were or were not significantly expressed in the developing limb bud. The specific localization of 53 selected candidates within the blastema was investigated by in situ hybridization. In summary, we identified a set of genes that are expressed specifically during regeneration and are therefore, likely candidates for the regulation of blastema formation. |
| format |
article |
| author |
Dunja Knapp Herbert Schulz Cynthia Alexander Rascon Michael Volkmer Juliane Scholz Eugen Nacu Mu Le Sergey Novozhilov Akira Tazaki Stephanie Protze Tina Jacob Norbert Hubner Bianca Habermann Elly M Tanaka |
| author_facet |
Dunja Knapp Herbert Schulz Cynthia Alexander Rascon Michael Volkmer Juliane Scholz Eugen Nacu Mu Le Sergey Novozhilov Akira Tazaki Stephanie Protze Tina Jacob Norbert Hubner Bianca Habermann Elly M Tanaka |
| author_sort |
Dunja Knapp |
| title |
Comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program. |
| title_short |
Comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program. |
| title_full |
Comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program. |
| title_fullStr |
Comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program. |
| title_full_unstemmed |
Comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program. |
| title_sort |
comparative transcriptional profiling of the axolotl limb identifies a tripartite regeneration-specific gene program. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/3dd2bd81bd9449bb9839b6659253921b |
| work_keys_str_mv |
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