Predominance of non-Streptococcus mutans bacteria in dental biofilm and its relation to caries progression

This study aims to assess differences in biofilm bacterial composition between patients with low and high caries. Patients without a medical problem and with no history of antibiotic use, mouth wash or fluoride application in the previous 3 months were recruited. Caries was recorded at cavitation le...

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Autores principales:	Doaa M. AlEraky, Marwa Madi, Maha El Tantawi, Jehan AlHumaid, Sarah Fita, Sayed AbdulAzeez, J. Francis Borgio, Fahad A. Al-Harbi, Adel S. Alagl
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Dental Caries Rothia Streptococcus 16S rRNA Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3dd67aba74ff41649441592c6b7ef943
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id	oai:doaj.org-article:3dd67aba74ff41649441592c6b7ef943
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spelling	oai:doaj.org-article:3dd67aba74ff41649441592c6b7ef9432021-11-20T04:57:11ZPredominance of non-Streptococcus mutans bacteria in dental biofilm and its relation to caries progression1319-562X10.1016/j.sjbs.2021.08.052https://doaj.org/article/3dd67aba74ff41649441592c6b7ef9432021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21007415https://doaj.org/toc/1319-562XThis study aims to assess differences in biofilm bacterial composition between patients with low and high caries. Patients without a medical problem and with no history of antibiotic use, mouth wash or fluoride application in the previous 3 months were recruited. Caries was recorded at cavitation level; score was calculated by a national mean (dmft of 4.8 and DMFT of 2.7). Pooled biofilm samples were collected from mesial, distal, buccal, lingual, and occlusal surfaces. Based on caries experience, individuals were classified into low and high caries and both groups were compared regarding bacteria identified using 16S rRNA gene sequencing, and molecular phylogenetic analysis of the isolates was performed. A total of twenty seven randomly selected samples with low (n = 13) and high (n = 14) caries. Identification of oral bacteria was performed using 16S rRNA sequence, Rothia mucilaginosa and R. aeria were identified in low caries individuals, while R. dentocariosa was detected in high caries individuals. Two Streptococcus spp. were identified only in low caries S. salivarius and S. gordonii whereas S. sanguinis, S. mitis, S. sinensis, S. rubneri, S. vestibularis, S. cristatus and S. massiliensis were identified only in individuals with high caries. This study revealed the absence of R. mucilaginosa in the high caries subjects and its coexistence with the low caries subjects. Streptococcus mutans was insignificant contributor of caries among samples, while, Streptococcus sanguinis was the main constituent of high caries Saudi patients.Doaa M. AlErakyMarwa MadiMaha El TantawiJehan AlHumaidSarah FitaSayed AbdulAzeezJ. Francis BorgioFahad A. Al-HarbiAdel S. AlaglElsevierarticleDental CariesRothiaStreptococcus16S rRNABiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7390-7395 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dental Caries Rothia Streptococcus 16S rRNA Biology (General) QH301-705.5
spellingShingle	Dental Caries Rothia Streptococcus 16S rRNA Biology (General) QH301-705.5 Doaa M. AlEraky Marwa Madi Maha El Tantawi Jehan AlHumaid Sarah Fita Sayed AbdulAzeez J. Francis Borgio Fahad A. Al-Harbi Adel S. Alagl Predominance of non-Streptococcus mutans bacteria in dental biofilm and its relation to caries progression
description	This study aims to assess differences in biofilm bacterial composition between patients with low and high caries. Patients without a medical problem and with no history of antibiotic use, mouth wash or fluoride application in the previous 3 months were recruited. Caries was recorded at cavitation level; score was calculated by a national mean (dmft of 4.8 and DMFT of 2.7). Pooled biofilm samples were collected from mesial, distal, buccal, lingual, and occlusal surfaces. Based on caries experience, individuals were classified into low and high caries and both groups were compared regarding bacteria identified using 16S rRNA gene sequencing, and molecular phylogenetic analysis of the isolates was performed. A total of twenty seven randomly selected samples with low (n = 13) and high (n = 14) caries. Identification of oral bacteria was performed using 16S rRNA sequence, Rothia mucilaginosa and R. aeria were identified in low caries individuals, while R. dentocariosa was detected in high caries individuals. Two Streptococcus spp. were identified only in low caries S. salivarius and S. gordonii whereas S. sanguinis, S. mitis, S. sinensis, S. rubneri, S. vestibularis, S. cristatus and S. massiliensis were identified only in individuals with high caries. This study revealed the absence of R. mucilaginosa in the high caries subjects and its coexistence with the low caries subjects. Streptococcus mutans was insignificant contributor of caries among samples, while, Streptococcus sanguinis was the main constituent of high caries Saudi patients.
format	article
author	Doaa M. AlEraky Marwa Madi Maha El Tantawi Jehan AlHumaid Sarah Fita Sayed AbdulAzeez J. Francis Borgio Fahad A. Al-Harbi Adel S. Alagl
author_facet	Doaa M. AlEraky Marwa Madi Maha El Tantawi Jehan AlHumaid Sarah Fita Sayed AbdulAzeez J. Francis Borgio Fahad A. Al-Harbi Adel S. Alagl
author_sort	Doaa M. AlEraky
title	Predominance of non-Streptococcus mutans bacteria in dental biofilm and its relation to caries progression
title_short	Predominance of non-Streptococcus mutans bacteria in dental biofilm and its relation to caries progression
title_full	Predominance of non-Streptococcus mutans bacteria in dental biofilm and its relation to caries progression
title_fullStr	Predominance of non-Streptococcus mutans bacteria in dental biofilm and its relation to caries progression
title_full_unstemmed	Predominance of non-Streptococcus mutans bacteria in dental biofilm and its relation to caries progression
title_sort	predominance of non-streptococcus mutans bacteria in dental biofilm and its relation to caries progression
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/3dd67aba74ff41649441592c6b7ef943
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Predominance of non-Streptococcus mutans bacteria in dental biofilm and its relation to caries progression

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