Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue

Abstract Intramuscular adipose is conducive to good pork quality, whereas subcutaneous adipose is considered as waste in pig production. So uncovering the regulation differences between these two adiposes is helpful to tissue-specific control of fat deposition. In this study, we found the sensitivit...

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Autores principales:	Weiwei Chu, Wei Wei, Haiyin Han, Ying Gao, Kaiqing Liu, Ye Tian, Zaohang Jiang, Lifan Zhang, Jie Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3dd854b15d0045ce985a54658aaacbd1
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spelling	oai:doaj.org-article:3dd854b15d0045ce985a54658aaacbd12021-12-02T11:53:08ZMuscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue10.1038/s41598-017-00615-92045-2322https://doaj.org/article/3dd854b15d0045ce985a54658aaacbd12017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00615-9https://doaj.org/toc/2045-2322Abstract Intramuscular adipose is conducive to good pork quality, whereas subcutaneous adipose is considered as waste in pig production. So uncovering the regulation differences between these two adiposes is helpful to tissue-specific control of fat deposition. In this study, we found the sensitivity to glucocorticoids (GCs) was lower in intramuscular adipocytes (IMA) compared with subcutaneous adipocytes (SA). Comparison of glucocorticoid receptor (GR) revealed that IMA had lower GR level which contributed to its reduced GCs sensitivity. Higher methylation levels of GR promotor 1-C and 1-H were detected in IMA compared with SA. GR expression decrease was also found in adipocytes when treated with muscle conditioned medium (MCM) in vitro, which resulted in significant inhibition of adipocytes proliferation and differentiation. Since abundant myostatin (MSTN) was detected in MCM by ELISA assay, we further investigated the effect of this myokine on adipocytes. MSTN treatment suppressed adipocytes GR expression, cell proliferation and differentiation, which mimicked the effects of MCM. The methylation levels of GR promotor 1-C and 1-H were also elevated after MSTN treatment. Our study reveals the role of GR in muscle fiber inhibition on intramuscular adipocytes, and identifies myostatin as a muscle-derived modulator for adipose GR level.Weiwei ChuWei WeiHaiyin HanYing GaoKaiqing LiuYe TianZaohang JiangLifan ZhangJie ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Weiwei Chu Wei Wei Haiyin Han Ying Gao Kaiqing Liu Ye Tian Zaohang Jiang Lifan Zhang Jie Chen Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue
description	Abstract Intramuscular adipose is conducive to good pork quality, whereas subcutaneous adipose is considered as waste in pig production. So uncovering the regulation differences between these two adiposes is helpful to tissue-specific control of fat deposition. In this study, we found the sensitivity to glucocorticoids (GCs) was lower in intramuscular adipocytes (IMA) compared with subcutaneous adipocytes (SA). Comparison of glucocorticoid receptor (GR) revealed that IMA had lower GR level which contributed to its reduced GCs sensitivity. Higher methylation levels of GR promotor 1-C and 1-H were detected in IMA compared with SA. GR expression decrease was also found in adipocytes when treated with muscle conditioned medium (MCM) in vitro, which resulted in significant inhibition of adipocytes proliferation and differentiation. Since abundant myostatin (MSTN) was detected in MCM by ELISA assay, we further investigated the effect of this myokine on adipocytes. MSTN treatment suppressed adipocytes GR expression, cell proliferation and differentiation, which mimicked the effects of MCM. The methylation levels of GR promotor 1-C and 1-H were also elevated after MSTN treatment. Our study reveals the role of GR in muscle fiber inhibition on intramuscular adipocytes, and identifies myostatin as a muscle-derived modulator for adipose GR level.
format	article
author	Weiwei Chu Wei Wei Haiyin Han Ying Gao Kaiqing Liu Ye Tian Zaohang Jiang Lifan Zhang Jie Chen
author_facet	Weiwei Chu Wei Wei Haiyin Han Ying Gao Kaiqing Liu Ye Tian Zaohang Jiang Lifan Zhang Jie Chen
author_sort	Weiwei Chu
title	Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue
title_short	Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue
title_full	Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue
title_fullStr	Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue
title_full_unstemmed	Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue
title_sort	muscle-specific downregulation of gr levels inhibits adipogenesis in porcine intramuscular adipocyte tissue
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3dd854b15d0045ce985a54658aaacbd1
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_version_	1718394862112342016

Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue

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