Beneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study

Abstract Psychological stress and occlusal alteration are important etiologic factors for temporomandibular/masticatory muscular disorders. In particular, the exact physiologic mechanism underlying the relation by occlusal alteration and temporomandibular disorders remains unclear. Our purpose was t...

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Autores principales:	Glauce C. Nascimento, Bruno L. Malzone, Daniela M. Iyomasa, Yamba C. L. Pereira, João Paulo M. Issa, Christie R. A. Leite-Panissi, Ii-Sei Watanabe, Mamie M. Iyomasa, Ramon Fuentes, Elaine Del Bel, Fernando J. Dias
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ddbe66fd75b46eb80b0882ffdad0fa0
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spelling	oai:doaj.org-article:3ddbe66fd75b46eb80b0882ffdad0fa02021-12-02T14:47:31ZBeneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study10.1038/s41598-020-65524-w2045-2322https://doaj.org/article/3ddbe66fd75b46eb80b0882ffdad0fa02020-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-65524-whttps://doaj.org/toc/2045-2322Abstract Psychological stress and occlusal alteration are important etiologic factors for temporomandibular/masticatory muscular disorders. In particular, the exact physiologic mechanism underlying the relation by occlusal alteration and temporomandibular disorders remains unclear. Our purpose was to test the hypothesis that benzodiazepine therapy is able to prevent metabolic and vascular changes in the medial pterygoid muscle of rats under chronic stress after 14 days of unilateral exodontia. Adult Wistar rats were submitted to unpredictable chronic mild stress (10 days) and/or unilateral exodontia and their plasma and medial pterygoid muscles were removed for analysis. A pre-treatment with diazepam was used to verify its effect on stress. The parameters evaluated included anxiety behavior, plasma levels of corticosterone, metabolic activity by succinate dehydrogenase, capillary density by laminin staining and ultrastructural findings by transmission electron microscopy. Occlusal instability induced anxiety-like behavior on elevated plus-maze test and diazepam administration blocked the appearance of this behavior. Unilateral exodontia promoted in the contralateral muscle an increase of oxidative fibers and capillaries and modification of sarcoplasmic reticulum. Chronic stress caused increased glycolytic metabolism, reduced capillary density and morphological changes in mitochondria on both sides. Association of both factors induced a glycolytic pattern in muscle and hemodynamic changes. Pharmacological manipulation with diazepam inhibited the changes in the medial pterygoid muscle after stress. Our results reveal a preventive benzodiazepine treatment for stress and occlusal instability conditions affecting masticatory muscle disorders. In addition, provide insights into the mechanisms by which chronic stress and exodontia might be involved in the pathophysiology of masticatory muscular dysfunctions.Glauce C. NascimentoBruno L. MalzoneDaniela M. IyomasaYamba C. L. PereiraJoão Paulo M. IssaChristie R. A. Leite-PanissiIi-Sei WatanabeMamie M. IyomasaRamon FuentesElaine Del BelFernando J. DiasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Glauce C. Nascimento Bruno L. Malzone Daniela M. Iyomasa Yamba C. L. Pereira João Paulo M. Issa Christie R. A. Leite-Panissi Ii-Sei Watanabe Mamie M. Iyomasa Ramon Fuentes Elaine Del Bel Fernando J. Dias Beneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study
description	Abstract Psychological stress and occlusal alteration are important etiologic factors for temporomandibular/masticatory muscular disorders. In particular, the exact physiologic mechanism underlying the relation by occlusal alteration and temporomandibular disorders remains unclear. Our purpose was to test the hypothesis that benzodiazepine therapy is able to prevent metabolic and vascular changes in the medial pterygoid muscle of rats under chronic stress after 14 days of unilateral exodontia. Adult Wistar rats were submitted to unpredictable chronic mild stress (10 days) and/or unilateral exodontia and their plasma and medial pterygoid muscles were removed for analysis. A pre-treatment with diazepam was used to verify its effect on stress. The parameters evaluated included anxiety behavior, plasma levels of corticosterone, metabolic activity by succinate dehydrogenase, capillary density by laminin staining and ultrastructural findings by transmission electron microscopy. Occlusal instability induced anxiety-like behavior on elevated plus-maze test and diazepam administration blocked the appearance of this behavior. Unilateral exodontia promoted in the contralateral muscle an increase of oxidative fibers and capillaries and modification of sarcoplasmic reticulum. Chronic stress caused increased glycolytic metabolism, reduced capillary density and morphological changes in mitochondria on both sides. Association of both factors induced a glycolytic pattern in muscle and hemodynamic changes. Pharmacological manipulation with diazepam inhibited the changes in the medial pterygoid muscle after stress. Our results reveal a preventive benzodiazepine treatment for stress and occlusal instability conditions affecting masticatory muscle disorders. In addition, provide insights into the mechanisms by which chronic stress and exodontia might be involved in the pathophysiology of masticatory muscular dysfunctions.
format	article
author	Glauce C. Nascimento Bruno L. Malzone Daniela M. Iyomasa Yamba C. L. Pereira João Paulo M. Issa Christie R. A. Leite-Panissi Ii-Sei Watanabe Mamie M. Iyomasa Ramon Fuentes Elaine Del Bel Fernando J. Dias
author_facet	Glauce C. Nascimento Bruno L. Malzone Daniela M. Iyomasa Yamba C. L. Pereira João Paulo M. Issa Christie R. A. Leite-Panissi Ii-Sei Watanabe Mamie M. Iyomasa Ramon Fuentes Elaine Del Bel Fernando J. Dias
author_sort	Glauce C. Nascimento
title	Beneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study
title_short	Beneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study
title_full	Beneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study
title_fullStr	Beneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study
title_full_unstemmed	Beneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study
title_sort	beneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/3ddbe66fd75b46eb80b0882ffdad0fa0
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Beneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study

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