Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy

(1) Background: The control of angiogenesis is essential in disease treatment. We investigated angiogenesis-promoting or -suppressing factors and their molecular mechanisms. (2) Methods: Angiogenesis from HUVECs was quantitatively analyzed using the Angiogenesis Analysis Kit (Kurabo, Osaka, Japan)....

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Autores principales:	Takahiro Shimazaki, Nobuhiro Noro, Kazuhiro Hagikura, Taro Matsumoto, Chikako Yoshida-Noro
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HUVECs angiogenesis Ang-1 VEGF flavonoid polyphenol Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3de7113a8937453692d92c07b45fe146
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spelling	oai:doaj.org-article:3de7113a8937453692d92c07b45fe1462021-11-25T16:48:09ZQuantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy10.3390/biology101112122079-7737https://doaj.org/article/3de7113a8937453692d92c07b45fe1462021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1212https://doaj.org/toc/2079-7737(1) Background: The control of angiogenesis is essential in disease treatment. We investigated angiogenesis-promoting or -suppressing factors and their molecular mechanisms. (2) Methods: Angiogenesis from HUVECs was quantitatively analyzed using the Angiogenesis Analysis Kit (Kurabo, Osaka, Japan). Human rAng-1-producing 107-35 CHO cells or mouse DFAT-D1 cells were co-cultured with HUVEC. Antioxidant polyphenols were added to the culture. Gene expression was analyzed by RT-PCR. (3) Results: The addition of rAng-1-producing cells, their culture supernatant, or commercially available rAng-1 showed a promoting effect on angiogenesis. The co-culture of DFAT-D1 cells promoted angiogenesis. Polyphenols showed a dose-dependent inhibitory effect on angiogenesis. Luteolin and quercetin showed remarkable anti-angiogenic effects. The expression of vWF, Flk1, and PECAM-1 was increased by adding rAng-1-producing cell culture supernatant. Polyphenols suppressed these genes. Apigenin and luteolin markedly suppressed α-SMA and Flk1. Resveratrol and quercetin enhanced the expression of PPARγ, and luteolin suppressed the expression of COX-1. The expression of endothelial nitric oxide synthase (eNOS), an oxidative stress-related gene, was slightly increased by luteolin. These results suggest that polyphenols induce ROS reduction. (4) Conclusions: We showed the promoting effect of Ang-1 or DFAT and the suppressing effect of polyphenols on angiogenesis and studied their molecular mechanisms. These results help control angiogenesis in regenerative therapy.Takahiro ShimazakiNobuhiro NoroKazuhiro HagikuraTaro MatsumotoChikako Yoshida-NoroMDPI AGarticleHUVECsangiogenesisAng-1VEGFflavonoidpolyphenolBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1212, p 1212 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HUVECs angiogenesis Ang-1 VEGF flavonoid polyphenol Biology (General) QH301-705.5
spellingShingle	HUVECs angiogenesis Ang-1 VEGF flavonoid polyphenol Biology (General) QH301-705.5 Takahiro Shimazaki Nobuhiro Noro Kazuhiro Hagikura Taro Matsumoto Chikako Yoshida-Noro Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy
description	(1) Background: The control of angiogenesis is essential in disease treatment. We investigated angiogenesis-promoting or -suppressing factors and their molecular mechanisms. (2) Methods: Angiogenesis from HUVECs was quantitatively analyzed using the Angiogenesis Analysis Kit (Kurabo, Osaka, Japan). Human rAng-1-producing 107-35 CHO cells or mouse DFAT-D1 cells were co-cultured with HUVEC. Antioxidant polyphenols were added to the culture. Gene expression was analyzed by RT-PCR. (3) Results: The addition of rAng-1-producing cells, their culture supernatant, or commercially available rAng-1 showed a promoting effect on angiogenesis. The co-culture of DFAT-D1 cells promoted angiogenesis. Polyphenols showed a dose-dependent inhibitory effect on angiogenesis. Luteolin and quercetin showed remarkable anti-angiogenic effects. The expression of vWF, Flk1, and PECAM-1 was increased by adding rAng-1-producing cell culture supernatant. Polyphenols suppressed these genes. Apigenin and luteolin markedly suppressed α-SMA and Flk1. Resveratrol and quercetin enhanced the expression of PPARγ, and luteolin suppressed the expression of COX-1. The expression of endothelial nitric oxide synthase (eNOS), an oxidative stress-related gene, was slightly increased by luteolin. These results suggest that polyphenols induce ROS reduction. (4) Conclusions: We showed the promoting effect of Ang-1 or DFAT and the suppressing effect of polyphenols on angiogenesis and studied their molecular mechanisms. These results help control angiogenesis in regenerative therapy.
format	article
author	Takahiro Shimazaki Nobuhiro Noro Kazuhiro Hagikura Taro Matsumoto Chikako Yoshida-Noro
author_facet	Takahiro Shimazaki Nobuhiro Noro Kazuhiro Hagikura Taro Matsumoto Chikako Yoshida-Noro
author_sort	Takahiro Shimazaki
title	Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy
title_short	Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy
title_full	Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy
title_fullStr	Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy
title_full_unstemmed	Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy
title_sort	quantitative analysis of factors regulating angiogenesis for stem cell therapy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3de7113a8937453692d92c07b45fe146
work_keys_str_mv	AT takahiroshimazaki quantitativeanalysisoffactorsregulatingangiogenesisforstemcelltherapy AT nobuhironoro quantitativeanalysisoffactorsregulatingangiogenesisforstemcelltherapy AT kazuhirohagikura quantitativeanalysisoffactorsregulatingangiogenesisforstemcelltherapy AT taromatsumoto quantitativeanalysisoffactorsregulatingangiogenesisforstemcelltherapy AT chikakoyoshidanoro quantitativeanalysisoffactorsregulatingangiogenesisforstemcelltherapy
_version_	1718412949007106048

Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy

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