Biomolecular phenotyping and heterogeneity assessment of mesenchymal stromal cells using label-free Raman spectroscopy

Abstract Easy, quantitative measures of biomolecular heterogeneity and high-stratified phenotyping are needed to identify and characterise complex disease processes at the single-cell level, as well as to predict cell fate. Here, we demonstrate how Raman spectroscopy can be used in the difficult-to-...

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Main Authors:	R. A. Rocha, J. M. Fox, P. G. Genever, Y. Hancock
Format:	article
Language:	EN
Published:	Nature Portfolio 2021
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/3decc8af6a5f46b288f59e860729de2f
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spelling	oai:doaj.org-article:3decc8af6a5f46b288f59e860729de2f2021-12-02T14:28:20ZBiomolecular phenotyping and heterogeneity assessment of mesenchymal stromal cells using label-free Raman spectroscopy10.1038/s41598-021-81991-12045-2322https://doaj.org/article/3decc8af6a5f46b288f59e860729de2f2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81991-1https://doaj.org/toc/2045-2322Abstract Easy, quantitative measures of biomolecular heterogeneity and high-stratified phenotyping are needed to identify and characterise complex disease processes at the single-cell level, as well as to predict cell fate. Here, we demonstrate how Raman spectroscopy can be used in the difficult-to-assess case of clonal, bone-derived mesenchymal stromal cells (MSCs) to identify MSC lines and group these according to biological function (e.g., differentiation capacity). Biomolecular stratification is achieved using high-precision measures obtained from representative statistical sampling that also enable quantified heterogeneity assessment. Application to primary MSCs and human dermal fibroblasts shows use of these measures as a label-free assay to classify cell sub-types within complex heterogeneous cell populations, thus demonstrating the potential for therapeutic translation, and broad application to the phenotypic characterisation of other cells.R. A. RochaJ. M. FoxP. G. GeneverY. HancockNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q R. A. Rocha J. M. Fox P. G. Genever Y. Hancock Biomolecular phenotyping and heterogeneity assessment of mesenchymal stromal cells using label-free Raman spectroscopy
description	Abstract Easy, quantitative measures of biomolecular heterogeneity and high-stratified phenotyping are needed to identify and characterise complex disease processes at the single-cell level, as well as to predict cell fate. Here, we demonstrate how Raman spectroscopy can be used in the difficult-to-assess case of clonal, bone-derived mesenchymal stromal cells (MSCs) to identify MSC lines and group these according to biological function (e.g., differentiation capacity). Biomolecular stratification is achieved using high-precision measures obtained from representative statistical sampling that also enable quantified heterogeneity assessment. Application to primary MSCs and human dermal fibroblasts shows use of these measures as a label-free assay to classify cell sub-types within complex heterogeneous cell populations, thus demonstrating the potential for therapeutic translation, and broad application to the phenotypic characterisation of other cells.
format	article
author	R. A. Rocha J. M. Fox P. G. Genever Y. Hancock
author_facet	R. A. Rocha J. M. Fox P. G. Genever Y. Hancock
author_sort	R. A. Rocha
title	Biomolecular phenotyping and heterogeneity assessment of mesenchymal stromal cells using label-free Raman spectroscopy
title_short	Biomolecular phenotyping and heterogeneity assessment of mesenchymal stromal cells using label-free Raman spectroscopy
title_full	Biomolecular phenotyping and heterogeneity assessment of mesenchymal stromal cells using label-free Raman spectroscopy
title_fullStr	Biomolecular phenotyping and heterogeneity assessment of mesenchymal stromal cells using label-free Raman spectroscopy
title_full_unstemmed	Biomolecular phenotyping and heterogeneity assessment of mesenchymal stromal cells using label-free Raman spectroscopy
title_sort	biomolecular phenotyping and heterogeneity assessment of mesenchymal stromal cells using label-free raman spectroscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3decc8af6a5f46b288f59e860729de2f
work_keys_str_mv	AT rarocha biomolecularphenotypingandheterogeneityassessmentofmesenchymalstromalcellsusinglabelfreeramanspectroscopy AT jmfox biomolecularphenotypingandheterogeneityassessmentofmesenchymalstromalcellsusinglabelfreeramanspectroscopy AT pggenever biomolecularphenotypingandheterogeneityassessmentofmesenchymalstromalcellsusinglabelfreeramanspectroscopy AT yhancock biomolecularphenotypingandheterogeneityassessmentofmesenchymalstromalcellsusinglabelfreeramanspectroscopy
_version_	1718391245696401408

Biomolecular phenotyping and heterogeneity assessment of mesenchymal stromal cells using label-free Raman spectroscopy

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