Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences

Abstract. Setyati WA, Martani E, Triyanto, Zainuddin M, Puspita M, Suryono CA, Subagyo, Pringgenies D. 2019. Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences. Biodiversitas 20...

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Autores principales:	wilis ari setyati, ERNI MARTANI, TRIYANTO, MUHAMMAD ZAINUDDIN, MAYA PUSPITA, CHRISNA ADI SURYONO, SUBAGYO, delianis pringgenies
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	16s-rrna gene, heterogeneity, mangrove, proteolytic bacteria, rep pcr Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3ded55db7ab542678b7a01f5a76559f3
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spelling	oai:doaj.org-article:3ded55db7ab542678b7a01f5a76559f32021-11-21T21:57:24ZGenetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences1412-033X2085-472210.13057/biodiv/d201118https://doaj.org/article/3ded55db7ab542678b7a01f5a76559f32019-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4227https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Setyati WA, Martani E, Triyanto, Zainuddin M, Puspita M, Suryono CA, Subagyo, Pringgenies D. 2019. Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences. Biodiversitas 20: 3256-3261. Intensive shrimp farming has organic waste that results in pollution. Such waste needs to be bio-remediated for liquid waste. This study aimed to discover bacteria isolated from mangrove sediments that can degrade organic matter and apply them for bioremediation of polluted shrimp farms. The study consisted of bacterial isolation, bioassay of enzymatic activity, and isolates identification through cluster analysis. Bacterial isolates were collected from mangrove ecosystems in Rembang (R), Cilacap (C), Banyuwangi (B), and Karimunjawa (K). Enzymatic activity test consists of proteolytic, amylolytic, cellulolytic, lipolytic, and ligninolytic activity. Identification analysis was conducted with 16S-rRNA gene sequences followed by cluster analysis using the results of Rep-PCR. There were 19 isolates derived from proteolytic mangrove area represented in five clusters (groups). Group 1 consisted of 5 isolates; isolates 14.C, 22.R, 28.K, 15.C and 19.R. Group 2 consisted of 5 isolates; 30.K, 33.K, 34.K, 39.K and 40.B. Group 3 consisted of 3 isolates of bacteria; 13.C, 2.C and 32.C. Group 4 consisted of 5 isolates; isolates 36.K, 35.K, 37.K, 38.K and 48.B. Group 5 consisted of 1 isolate was isolate 26.R. The results of the sequences analysis of the 16S-rRNA gene indicated that the isolate 13C of the Group 3 had 97% sequence homology with Bacillus oceanisediminis strain H2. Isolate 14.C of the Group 1 had 96% sequence homology with Halomonas aquamarina strain DSM 30161. Isolate 26.R of the Group 5 had 98% sequence homology with Acinetobacter pitti strain ATCC 19004. Isolate 30.K of the Group 2 had 93% sequence homology with Salinicola salarius strain M27. Isolate 36.K of the Group 4 had 97% sequence homology with Bacillus aquimaris strain TF-12. Please write here your concluding remarks based on your results obtained.wilis ari setyatiERNI MARTANITRIYANTOMUHAMMAD ZAINUDDINMAYA PUSPITACHRISNA ADI SURYONOSUBAGYOdelianis pringgeniesMBI & UNS Soloarticle16s-rrna gene, heterogeneity, mangrove, proteolytic bacteria, rep pcrBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s-rrna gene, heterogeneity, mangrove, proteolytic bacteria, rep pcr Biology (General) QH301-705.5
spellingShingle	16s-rrna gene, heterogeneity, mangrove, proteolytic bacteria, rep pcr Biology (General) QH301-705.5 wilis ari setyati ERNI MARTANI TRIYANTO MUHAMMAD ZAINUDDIN MAYA PUSPITA CHRISNA ADI SURYONO SUBAGYO delianis pringgenies Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences
description	Abstract. Setyati WA, Martani E, Triyanto, Zainuddin M, Puspita M, Suryono CA, Subagyo, Pringgenies D. 2019. Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences. Biodiversitas 20: 3256-3261. Intensive shrimp farming has organic waste that results in pollution. Such waste needs to be bio-remediated for liquid waste. This study aimed to discover bacteria isolated from mangrove sediments that can degrade organic matter and apply them for bioremediation of polluted shrimp farms. The study consisted of bacterial isolation, bioassay of enzymatic activity, and isolates identification through cluster analysis. Bacterial isolates were collected from mangrove ecosystems in Rembang (R), Cilacap (C), Banyuwangi (B), and Karimunjawa (K). Enzymatic activity test consists of proteolytic, amylolytic, cellulolytic, lipolytic, and ligninolytic activity. Identification analysis was conducted with 16S-rRNA gene sequences followed by cluster analysis using the results of Rep-PCR. There were 19 isolates derived from proteolytic mangrove area represented in five clusters (groups). Group 1 consisted of 5 isolates; isolates 14.C, 22.R, 28.K, 15.C and 19.R. Group 2 consisted of 5 isolates; 30.K, 33.K, 34.K, 39.K and 40.B. Group 3 consisted of 3 isolates of bacteria; 13.C, 2.C and 32.C. Group 4 consisted of 5 isolates; isolates 36.K, 35.K, 37.K, 38.K and 48.B. Group 5 consisted of 1 isolate was isolate 26.R. The results of the sequences analysis of the 16S-rRNA gene indicated that the isolate 13C of the Group 3 had 97% sequence homology with Bacillus oceanisediminis strain H2. Isolate 14.C of the Group 1 had 96% sequence homology with Halomonas aquamarina strain DSM 30161. Isolate 26.R of the Group 5 had 98% sequence homology with Acinetobacter pitti strain ATCC 19004. Isolate 30.K of the Group 2 had 93% sequence homology with Salinicola salarius strain M27. Isolate 36.K of the Group 4 had 97% sequence homology with Bacillus aquimaris strain TF-12. Please write here your concluding remarks based on your results obtained.
format	article
author	wilis ari setyati ERNI MARTANI TRIYANTO MUHAMMAD ZAINUDDIN MAYA PUSPITA CHRISNA ADI SURYONO SUBAGYO delianis pringgenies
author_facet	wilis ari setyati ERNI MARTANI TRIYANTO MUHAMMAD ZAINUDDIN MAYA PUSPITA CHRISNA ADI SURYONO SUBAGYO delianis pringgenies
author_sort	wilis ari setyati
title	Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences
title_short	Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences
title_full	Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences
title_fullStr	Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences
title_full_unstemmed	Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences
title_sort	genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16s-rrna gene sequences
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/3ded55db7ab542678b7a01f5a76559f3
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Genetic heterogeneity of proteolytic bacteria isolated from sediments mangrove areas based on repetitive sequence-based polymerase chain reaction and 16S-rRNA gene sequences

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