Multiparametric magnetic resonance imaging features of a canine glioblastoma model.

<h4>Purpose</h4>To assess glioblastoma multiforme (GBM) formation with similar imaging characteristics to human GBM using multiparametric magnetic resonance imaging (MRI) in an orthotopic xenograft canine GBM model.<h4>Materials and methods</h4>The canine GBM cell line J3T1 w...

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Autores principales:	Seunghyun Lee, Seung Hong Choi, Hye Rim Cho, Jaemoon Koh, Chul-Kee Park, Tomotsugu Ichikawa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3dfaab704f2c475890e660b519595baa
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spelling	oai:doaj.org-article:3dfaab704f2c475890e660b519595baa2021-12-02T20:15:31ZMultiparametric magnetic resonance imaging features of a canine glioblastoma model.1932-620310.1371/journal.pone.0254448https://doaj.org/article/3dfaab704f2c475890e660b519595baa2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254448https://doaj.org/toc/1932-6203<h4>Purpose</h4>To assess glioblastoma multiforme (GBM) formation with similar imaging characteristics to human GBM using multiparametric magnetic resonance imaging (MRI) in an orthotopic xenograft canine GBM model.<h4>Materials and methods</h4>The canine GBM cell line J3T1 was subcutaneously injected into 6-week-old female BALB/c nude mice to obtain tumour fragments. Tumour fragments were implanted into adult male mongrel dog brains through surgery. Multiparametric MRI was performed with conventional MRI, diffusion-weighted imaging, and dynamic susceptibility contrast-enhanced perfusion-weighted imaging at one week and two weeks after surgery in a total of 15 surgical success cases. The presence of tumour cells, the necrotic area fraction, and the microvessel density (MVD) of the tumour on the histologic specimen were assessed. Tumour volume, diffusion, and perfusion parameters were compared at each time point using Wilcoxon signed-rank tests, and the differences between tumour and normal parenchyma were compared using unpaired t-tests. Spearman correlation analysis was performed between the imaging and histologic parameters.<h4>Results</h4>All animals showed a peripheral enhancing lesion on MRI and confirmed the presence of a tumour through histologic analysis (92.3%). The normalized perfusion values did not show significant decreases through at least 2 weeks after the surgery (P > 0.05). There was greater cerebral blood volume and flow in the GBM than in the normal-appearing white matter (1.46 ± 0.25 vs. 1.13 ± 0.16 and 1.30 ± 0.22 vs. 1.02 ± 0.14; P < 0.001 and P < 0.001, respectively). The MVD in the histologic specimens was correlated with the cerebral blood volume in the GBM tissue (r = 0.850, P = 0.004).<h4>Conclusion</h4>Our results suggest that the canine GBM model showed perfusion imaging characteristics similar to those of humans, and it might have potential as a model to assess novel technical developments for GBM treatment.Seunghyun LeeSeung Hong ChoiHye Rim ChoJaemoon KohChul-Kee ParkTomotsugu IchikawaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254448 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seunghyun Lee Seung Hong Choi Hye Rim Cho Jaemoon Koh Chul-Kee Park Tomotsugu Ichikawa Multiparametric magnetic resonance imaging features of a canine glioblastoma model.
description	<h4>Purpose</h4>To assess glioblastoma multiforme (GBM) formation with similar imaging characteristics to human GBM using multiparametric magnetic resonance imaging (MRI) in an orthotopic xenograft canine GBM model.<h4>Materials and methods</h4>The canine GBM cell line J3T1 was subcutaneously injected into 6-week-old female BALB/c nude mice to obtain tumour fragments. Tumour fragments were implanted into adult male mongrel dog brains through surgery. Multiparametric MRI was performed with conventional MRI, diffusion-weighted imaging, and dynamic susceptibility contrast-enhanced perfusion-weighted imaging at one week and two weeks after surgery in a total of 15 surgical success cases. The presence of tumour cells, the necrotic area fraction, and the microvessel density (MVD) of the tumour on the histologic specimen were assessed. Tumour volume, diffusion, and perfusion parameters were compared at each time point using Wilcoxon signed-rank tests, and the differences between tumour and normal parenchyma were compared using unpaired t-tests. Spearman correlation analysis was performed between the imaging and histologic parameters.<h4>Results</h4>All animals showed a peripheral enhancing lesion on MRI and confirmed the presence of a tumour through histologic analysis (92.3%). The normalized perfusion values did not show significant decreases through at least 2 weeks after the surgery (P > 0.05). There was greater cerebral blood volume and flow in the GBM than in the normal-appearing white matter (1.46 ± 0.25 vs. 1.13 ± 0.16 and 1.30 ± 0.22 vs. 1.02 ± 0.14; P < 0.001 and P < 0.001, respectively). The MVD in the histologic specimens was correlated with the cerebral blood volume in the GBM tissue (r = 0.850, P = 0.004).<h4>Conclusion</h4>Our results suggest that the canine GBM model showed perfusion imaging characteristics similar to those of humans, and it might have potential as a model to assess novel technical developments for GBM treatment.
format	article
author	Seunghyun Lee Seung Hong Choi Hye Rim Cho Jaemoon Koh Chul-Kee Park Tomotsugu Ichikawa
author_facet	Seunghyun Lee Seung Hong Choi Hye Rim Cho Jaemoon Koh Chul-Kee Park Tomotsugu Ichikawa
author_sort	Seunghyun Lee
title	Multiparametric magnetic resonance imaging features of a canine glioblastoma model.
title_short	Multiparametric magnetic resonance imaging features of a canine glioblastoma model.
title_full	Multiparametric magnetic resonance imaging features of a canine glioblastoma model.
title_fullStr	Multiparametric magnetic resonance imaging features of a canine glioblastoma model.
title_full_unstemmed	Multiparametric magnetic resonance imaging features of a canine glioblastoma model.
title_sort	multiparametric magnetic resonance imaging features of a canine glioblastoma model.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3dfaab704f2c475890e660b519595baa
work_keys_str_mv	AT seunghyunlee multiparametricmagneticresonanceimagingfeaturesofacanineglioblastomamodel AT seunghongchoi multiparametricmagneticresonanceimagingfeaturesofacanineglioblastomamodel AT hyerimcho multiparametricmagneticresonanceimagingfeaturesofacanineglioblastomamodel AT jaemoonkoh multiparametricmagneticresonanceimagingfeaturesofacanineglioblastomamodel AT chulkeepark multiparametricmagneticresonanceimagingfeaturesofacanineglioblastomamodel AT tomotsuguichikawa multiparametricmagneticresonanceimagingfeaturesofacanineglioblastomamodel
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Multiparametric magnetic resonance imaging features of a canine glioblastoma model.

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