Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens

Nasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite for pneumococcal transmission and disease. Current vaccines protect only against disease and colonization caused by a limited number of serotypes, consequently allowing serotype replacement and transmission. Therefore, the dev...

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Autores principales: Lucille F. van Beek, Kristin Surmann, H. Bart van den Berg van Saparoea, Diane Houben, Wouter S. P. Jong, Christian Hentschker, Thomas H. A. Ederveen, Elena Mitsi, Daniela M. Ferreira, Fred van Opzeeland, Christa E. van der Gaast – de Jongh, Irma Joosten, Uwe Völker, Frank Schmidt, Joen Luirink, Dimitri A. Diavatopoulos, Marien I. de Jonge
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Publicado: Taylor & Francis Group 2020
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Acceso en línea:https://doaj.org/article/3e016418b8e44a38bee691ecfa6505c3
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spelling oai:doaj.org-article:3e016418b8e44a38bee691ecfa6505c32021-11-17T14:21:59ZExploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens2150-55942150-560810.1080/21505594.2020.1825908https://doaj.org/article/3e016418b8e44a38bee691ecfa6505c32020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1825908https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Nasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite for pneumococcal transmission and disease. Current vaccines protect only against disease and colonization caused by a limited number of serotypes, consequently allowing serotype replacement and transmission. Therefore, the development of a broadly protective vaccine against colonization, transmission and disease is desired but requires a better understanding of pneumococcal adaptation to its natural niche. Hence, we measured the levels of free and protein-bound transition metals in human nasal fluid, to determine the effect of metal concentrations on the growth and proteome of S. pneumoniae. Pneumococci cultured in medium containing metal levels comparable to nasal fluid showed a highly distinct proteomic profile compared to standard culture conditions, including the increased abundance of nine conserved, putative surface-exposed proteins. AliA, an oligopeptide binding protein, was identified as the strongest protective antigen, demonstrated by the significantly reduced bacterial load in a murine colonization and a lethal mouse pneumonia model, highlighting its potential as vaccine antigen.Lucille F. van BeekKristin SurmannH. Bart van den Berg van SaparoeaDiane HoubenWouter S. P. JongChristian HentschkerThomas H. A. EderveenElena MitsiDaniela M. FerreiraFred van OpzeelandChrista E. van der Gaast – de JonghIrma JoostenUwe VölkerFrank SchmidtJoen LuirinkDimitri A. DiavatopoulosMarien I. de JongeTaylor & Francis Grouparticlestreptococcus pneumoniaetransition metalsnasal fluidprotein antigenscolonizationin vivo-mimickingInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1310-1328 (2020)
institution DOAJ
collection DOAJ
language EN
topic streptococcus pneumoniae
transition metals
nasal fluid
protein antigens
colonization
in vivo-mimicking
Infectious and parasitic diseases
RC109-216
spellingShingle streptococcus pneumoniae
transition metals
nasal fluid
protein antigens
colonization
in vivo-mimicking
Infectious and parasitic diseases
RC109-216
Lucille F. van Beek
Kristin Surmann
H. Bart van den Berg van Saparoea
Diane Houben
Wouter S. P. Jong
Christian Hentschker
Thomas H. A. Ederveen
Elena Mitsi
Daniela M. Ferreira
Fred van Opzeeland
Christa E. van der Gaast – de Jongh
Irma Joosten
Uwe Völker
Frank Schmidt
Joen Luirink
Dimitri A. Diavatopoulos
Marien I. de Jonge
Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens
description Nasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite for pneumococcal transmission and disease. Current vaccines protect only against disease and colonization caused by a limited number of serotypes, consequently allowing serotype replacement and transmission. Therefore, the development of a broadly protective vaccine against colonization, transmission and disease is desired but requires a better understanding of pneumococcal adaptation to its natural niche. Hence, we measured the levels of free and protein-bound transition metals in human nasal fluid, to determine the effect of metal concentrations on the growth and proteome of S. pneumoniae. Pneumococci cultured in medium containing metal levels comparable to nasal fluid showed a highly distinct proteomic profile compared to standard culture conditions, including the increased abundance of nine conserved, putative surface-exposed proteins. AliA, an oligopeptide binding protein, was identified as the strongest protective antigen, demonstrated by the significantly reduced bacterial load in a murine colonization and a lethal mouse pneumonia model, highlighting its potential as vaccine antigen.
format article
author Lucille F. van Beek
Kristin Surmann
H. Bart van den Berg van Saparoea
Diane Houben
Wouter S. P. Jong
Christian Hentschker
Thomas H. A. Ederveen
Elena Mitsi
Daniela M. Ferreira
Fred van Opzeeland
Christa E. van der Gaast – de Jongh
Irma Joosten
Uwe Völker
Frank Schmidt
Joen Luirink
Dimitri A. Diavatopoulos
Marien I. de Jonge
author_facet Lucille F. van Beek
Kristin Surmann
H. Bart van den Berg van Saparoea
Diane Houben
Wouter S. P. Jong
Christian Hentschker
Thomas H. A. Ederveen
Elena Mitsi
Daniela M. Ferreira
Fred van Opzeeland
Christa E. van der Gaast – de Jongh
Irma Joosten
Uwe Völker
Frank Schmidt
Joen Luirink
Dimitri A. Diavatopoulos
Marien I. de Jonge
author_sort Lucille F. van Beek
title Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens
title_short Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens
title_full Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens
title_fullStr Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens
title_full_unstemmed Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens
title_sort exploring metal availability in the natural niche of streptococcus pneumoniae to discover potential vaccine antigens
publisher Taylor & Francis Group
publishDate 2020
url https://doaj.org/article/3e016418b8e44a38bee691ecfa6505c3
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