A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold

Proteins smaller than about 50 kDa are currently too small to be imaged at high resolution by cryo‐electron microscopy (cryo‐EM). Here authors design a protein scaffold that binds 12 copies of a small 26 kDa protein (GFP), which allowed visualizing GFP at a resolution of 3.8Å by cryo‐EM.

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Detalles Bibliográficos
Autores principales:	Yuxi Liu, Duc T. Huynh, Todd O. Yeates
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3e07cb910a3049f3b5bdc9609bc1350e
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id	oai:doaj.org-article:3e07cb910a3049f3b5bdc9609bc1350e
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spelling	oai:doaj.org-article:3e07cb910a3049f3b5bdc9609bc1350e2021-12-02T16:57:44ZA 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold10.1038/s41467-019-09836-02041-1723https://doaj.org/article/3e07cb910a3049f3b5bdc9609bc1350e2019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09836-0https://doaj.org/toc/2041-1723Proteins smaller than about 50 kDa are currently too small to be imaged at high resolution by cryo‐electron microscopy (cryo‐EM). Here authors design a protein scaffold that binds 12 copies of a small 26 kDa protein (GFP), which allowed visualizing GFP at a resolution of 3.8Å by cryo‐EM.Yuxi LiuDuc T. HuynhTodd O. YeatesNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-7 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yuxi Liu Duc T. Huynh Todd O. Yeates A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold
description	Proteins smaller than about 50 kDa are currently too small to be imaged at high resolution by cryo‐electron microscopy (cryo‐EM). Here authors design a protein scaffold that binds 12 copies of a small 26 kDa protein (GFP), which allowed visualizing GFP at a resolution of 3.8Å by cryo‐EM.
format	article
author	Yuxi Liu Duc T. Huynh Todd O. Yeates
author_facet	Yuxi Liu Duc T. Huynh Todd O. Yeates
author_sort	Yuxi Liu
title	A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold
title_short	A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold
title_full	A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold
title_fullStr	A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold
title_full_unstemmed	A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold
title_sort	3.8 å resolution cryo-em structure of a small protein bound to an imaging scaffold
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/3e07cb910a3049f3b5bdc9609bc1350e
work_keys_str_mv	AT yuxiliu a38aresolutioncryoemstructureofasmallproteinboundtoanimagingscaffold AT ducthuynh a38aresolutioncryoemstructureofasmallproteinboundtoanimagingscaffold AT toddoyeates a38aresolutioncryoemstructureofasmallproteinboundtoanimagingscaffold AT yuxiliu 38aresolutioncryoemstructureofasmallproteinboundtoanimagingscaffold AT ducthuynh 38aresolutioncryoemstructureofasmallproteinboundtoanimagingscaffold AT toddoyeates 38aresolutioncryoemstructureofasmallproteinboundtoanimagingscaffold
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A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold

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