Increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration

Abstract The low in vivo survival rate of scaffold-seeded cells is still a challenge in stem cell-based bone regeneration. This study seeks to use a silk hydrogel to deliver more stem cells into a bone defect area and prolong the viability of these cells after implantation. Rat bone marrow stem cell...

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Autores principales:	Xun Ding, Guangzheng Yang, Wenjie Zhang, Guanglong Li, Shuxian Lin, David L. Kaplan, Xinquan Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3e0de7e292884c87a1054639ef0920e2
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spelling	oai:doaj.org-article:3e0de7e292884c87a1054639ef0920e22021-12-02T16:06:16ZIncreased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration10.1038/s41598-017-02053-z2045-2322https://doaj.org/article/3e0de7e292884c87a1054639ef0920e22017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02053-zhttps://doaj.org/toc/2045-2322Abstract The low in vivo survival rate of scaffold-seeded cells is still a challenge in stem cell-based bone regeneration. This study seeks to use a silk hydrogel to deliver more stem cells into a bone defect area and prolong the viability of these cells after implantation. Rat bone marrow stem cells were mingled with silk hydrogels at the concentrations of 1.0 × 105/mL, 1.0 × 106/mL and 1.0 × 107/mL before gelation, added dropwise to a silk scaffold and applied to a rat calvarial defect. A cell tracing experiment was included to observe the preservation of cell viability and function. The results show that the hydrogel with 1.0 × 107/mL stem cells exhibited the best osteogenic effect both in vitro and in vivo. The cell-tracing experiment shows that cells in the 1.0 × 107 group still survive and actively participate in new bone formation 8 weeks after implantation. The strategy of pre-mingling stem cells with the hydrogel had the effect of delivering more stem cells for bone engineering while preserving the viability and functions of these cells in vivo.Xun DingGuangzheng YangWenjie ZhangGuanglong LiShuxian LinDavid L. KaplanXinquan JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xun Ding Guangzheng Yang Wenjie Zhang Guanglong Li Shuxian Lin David L. Kaplan Xinquan Jiang Increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration
description	Abstract The low in vivo survival rate of scaffold-seeded cells is still a challenge in stem cell-based bone regeneration. This study seeks to use a silk hydrogel to deliver more stem cells into a bone defect area and prolong the viability of these cells after implantation. Rat bone marrow stem cells were mingled with silk hydrogels at the concentrations of 1.0 × 105/mL, 1.0 × 106/mL and 1.0 × 107/mL before gelation, added dropwise to a silk scaffold and applied to a rat calvarial defect. A cell tracing experiment was included to observe the preservation of cell viability and function. The results show that the hydrogel with 1.0 × 107/mL stem cells exhibited the best osteogenic effect both in vitro and in vivo. The cell-tracing experiment shows that cells in the 1.0 × 107 group still survive and actively participate in new bone formation 8 weeks after implantation. The strategy of pre-mingling stem cells with the hydrogel had the effect of delivering more stem cells for bone engineering while preserving the viability and functions of these cells in vivo.
format	article
author	Xun Ding Guangzheng Yang Wenjie Zhang Guanglong Li Shuxian Lin David L. Kaplan Xinquan Jiang
author_facet	Xun Ding Guangzheng Yang Wenjie Zhang Guanglong Li Shuxian Lin David L. Kaplan Xinquan Jiang
author_sort	Xun Ding
title	Increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration
title_short	Increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration
title_full	Increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration
title_fullStr	Increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration
title_full_unstemmed	Increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration
title_sort	increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3e0de7e292884c87a1054639ef0920e2
work_keys_str_mv	AT xunding increasedstemcellsdeliveredusingasilkgelscaffoldcomplexforenhancedboneregeneration AT guangzhengyang increasedstemcellsdeliveredusingasilkgelscaffoldcomplexforenhancedboneregeneration AT wenjiezhang increasedstemcellsdeliveredusingasilkgelscaffoldcomplexforenhancedboneregeneration AT guanglongli increasedstemcellsdeliveredusingasilkgelscaffoldcomplexforenhancedboneregeneration AT shuxianlin increasedstemcellsdeliveredusingasilkgelscaffoldcomplexforenhancedboneregeneration AT davidlkaplan increasedstemcellsdeliveredusingasilkgelscaffoldcomplexforenhancedboneregeneration AT xinquanjiang increasedstemcellsdeliveredusingasilkgelscaffoldcomplexforenhancedboneregeneration
_version_	1718385017560760320

Increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration

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