Genetic diversity may help evolutionary rescue in a clonal endemic plant species of Western Himalaya

Abstract Habitat loss due to climate change may cause the extinction of the clonal species with a limited distribution range. Thus, determining the genetic diversity required for adaptability by these species in sensitive ecosystems can help infer the chances of their survival and spread in changing...

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Auteurs principaux:	Irshad Ahmad Sofi, Irfan Rashid, Javaid Yousuf Lone, Sandhya Tyagi, Zafar A. Reshi, Reyazul Rouf Mir
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/3e12dcfec88d4896b0999fc5cd5cc432
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spelling	oai:doaj.org-article:3e12dcfec88d4896b0999fc5cd5cc4322021-12-02T17:17:39ZGenetic diversity may help evolutionary rescue in a clonal endemic plant species of Western Himalaya10.1038/s41598-021-98648-82045-2322https://doaj.org/article/3e12dcfec88d4896b0999fc5cd5cc4322021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98648-8https://doaj.org/toc/2045-2322Abstract Habitat loss due to climate change may cause the extinction of the clonal species with a limited distribution range. Thus, determining the genetic diversity required for adaptability by these species in sensitive ecosystems can help infer the chances of their survival and spread in changing climate. We studied the genetic diversity and population structure of Sambucus wightiana—a clonal endemic plant species of the Himalayan region for understanding its possible survival chances in anticipated climate change. Eight polymorphic microsatellite markers were used to study the allelic/genetic diversity and population structure. In addition, ITS1–ITS4 Sanger sequencing was used for phylogeny and SNP detection. A total number of 73 alleles were scored for 37 genotypes at 17 loci for 8 SSRs markers. The population structural analysis using the SSR marker data led to identifying two sub-populations in our collection of 37 S. wightiana genotypes, with 11 genotypes having mixed ancestry. The ITS sequence data show a specific allele in higher frequency in a particular sub-population, indicating variation in different S. wightiana accessions at the sequence level. The genotypic data of SSR markers and trait data of 11 traits of S. wightiana, when analyzed together, revealed five significant Marker-Trait Associations (MTAs) through Single Marker Analysis (SMA) or regression analysis. Most of the SSR markers were found to be associated with more than one trait, indicating the usefulness of these markers for working out marker-trait associations. Moderate to high genetic diversity observed in the present study may provide insurance against climate change to S. wightiana and help its further spread.Irshad Ahmad SofiIrfan RashidJavaid Yousuf LoneSandhya TyagiZafar A. ReshiReyazul Rouf MirNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Irshad Ahmad Sofi Irfan Rashid Javaid Yousuf Lone Sandhya Tyagi Zafar A. Reshi Reyazul Rouf Mir Genetic diversity may help evolutionary rescue in a clonal endemic plant species of Western Himalaya
description	Abstract Habitat loss due to climate change may cause the extinction of the clonal species with a limited distribution range. Thus, determining the genetic diversity required for adaptability by these species in sensitive ecosystems can help infer the chances of their survival and spread in changing climate. We studied the genetic diversity and population structure of Sambucus wightiana—a clonal endemic plant species of the Himalayan region for understanding its possible survival chances in anticipated climate change. Eight polymorphic microsatellite markers were used to study the allelic/genetic diversity and population structure. In addition, ITS1–ITS4 Sanger sequencing was used for phylogeny and SNP detection. A total number of 73 alleles were scored for 37 genotypes at 17 loci for 8 SSRs markers. The population structural analysis using the SSR marker data led to identifying two sub-populations in our collection of 37 S. wightiana genotypes, with 11 genotypes having mixed ancestry. The ITS sequence data show a specific allele in higher frequency in a particular sub-population, indicating variation in different S. wightiana accessions at the sequence level. The genotypic data of SSR markers and trait data of 11 traits of S. wightiana, when analyzed together, revealed five significant Marker-Trait Associations (MTAs) through Single Marker Analysis (SMA) or regression analysis. Most of the SSR markers were found to be associated with more than one trait, indicating the usefulness of these markers for working out marker-trait associations. Moderate to high genetic diversity observed in the present study may provide insurance against climate change to S. wightiana and help its further spread.
format	article
author	Irshad Ahmad Sofi Irfan Rashid Javaid Yousuf Lone Sandhya Tyagi Zafar A. Reshi Reyazul Rouf Mir
author_facet	Irshad Ahmad Sofi Irfan Rashid Javaid Yousuf Lone Sandhya Tyagi Zafar A. Reshi Reyazul Rouf Mir
author_sort	Irshad Ahmad Sofi
title	Genetic diversity may help evolutionary rescue in a clonal endemic plant species of Western Himalaya
title_short	Genetic diversity may help evolutionary rescue in a clonal endemic plant species of Western Himalaya
title_full	Genetic diversity may help evolutionary rescue in a clonal endemic plant species of Western Himalaya
title_fullStr	Genetic diversity may help evolutionary rescue in a clonal endemic plant species of Western Himalaya
title_full_unstemmed	Genetic diversity may help evolutionary rescue in a clonal endemic plant species of Western Himalaya
title_sort	genetic diversity may help evolutionary rescue in a clonal endemic plant species of western himalaya
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3e12dcfec88d4896b0999fc5cd5cc432
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Genetic diversity may help evolutionary rescue in a clonal endemic plant species of Western Himalaya

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