Kaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.

Kaposi's sarcoma herpesvirus (KSHV) encodes a cluster of twelve micro (mi)RNAs, which are abundantly expressed during both latent and lytic infection. Previous studies reported that KSHV is able to inhibit apoptosis during latent infection; we thus tested the involvement of viral miRNAs in this...

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Autores principales:	Guillaume Suffert, Georg Malterer, Jean Hausser, Johanna Viiliäinen, Aurélie Fender, Maud Contrant, Tomi Ivacevic, Vladimir Benes, Frédéric Gros, Olivier Voinnet, Mihaela Zavolan, Päivi M Ojala, Juergen G Haas, Sébastien Pfeffer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3e1319a9b25b4d58809a8612eb7109b1
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spelling	oai:doaj.org-article:3e1319a9b25b4d58809a8612eb7109b12021-11-18T06:05:01ZKaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.1553-73661553-737410.1371/journal.ppat.1002405https://doaj.org/article/3e1319a9b25b4d58809a8612eb7109b12011-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22174674/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Kaposi's sarcoma herpesvirus (KSHV) encodes a cluster of twelve micro (mi)RNAs, which are abundantly expressed during both latent and lytic infection. Previous studies reported that KSHV is able to inhibit apoptosis during latent infection; we thus tested the involvement of viral miRNAs in this process. We found that both HEK293 epithelial cells and DG75 cells stably expressing KSHV miRNAs were protected from apoptosis. Potential cellular targets that were significantly down-regulated upon KSHV miRNAs expression were identified by microarray profiling. Among them, we validated by luciferase reporter assays, quantitative PCR and western blotting caspase 3 (Casp3), a critical factor for the control of apoptosis. Using site-directed mutagenesis, we found that three KSHV miRNAs, miR-K12-1, 3 and 4-3p, were responsible for the targeting of Casp3. Specific inhibition of these miRNAs in KSHV-infected cells resulted in increased expression levels of endogenous Casp3 and enhanced apoptosis. Altogether, our results suggest that KSHV miRNAs directly participate in the previously reported inhibition of apoptosis by the virus, and are thus likely to play a role in KSHV-induced oncogenesis.Guillaume SuffertGeorg MaltererJean HausserJohanna ViiliäinenAurélie FenderMaud ContrantTomi IvacevicVladimir BenesFrédéric GrosOlivier VoinnetMihaela ZavolanPäivi M OjalaJuergen G HaasSébastien PfefferPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 12, p e1002405 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Guillaume Suffert Georg Malterer Jean Hausser Johanna Viiliäinen Aurélie Fender Maud Contrant Tomi Ivacevic Vladimir Benes Frédéric Gros Olivier Voinnet Mihaela Zavolan Päivi M Ojala Juergen G Haas Sébastien Pfeffer Kaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.
description	Kaposi's sarcoma herpesvirus (KSHV) encodes a cluster of twelve micro (mi)RNAs, which are abundantly expressed during both latent and lytic infection. Previous studies reported that KSHV is able to inhibit apoptosis during latent infection; we thus tested the involvement of viral miRNAs in this process. We found that both HEK293 epithelial cells and DG75 cells stably expressing KSHV miRNAs were protected from apoptosis. Potential cellular targets that were significantly down-regulated upon KSHV miRNAs expression were identified by microarray profiling. Among them, we validated by luciferase reporter assays, quantitative PCR and western blotting caspase 3 (Casp3), a critical factor for the control of apoptosis. Using site-directed mutagenesis, we found that three KSHV miRNAs, miR-K12-1, 3 and 4-3p, were responsible for the targeting of Casp3. Specific inhibition of these miRNAs in KSHV-infected cells resulted in increased expression levels of endogenous Casp3 and enhanced apoptosis. Altogether, our results suggest that KSHV miRNAs directly participate in the previously reported inhibition of apoptosis by the virus, and are thus likely to play a role in KSHV-induced oncogenesis.
format	article
author	Guillaume Suffert Georg Malterer Jean Hausser Johanna Viiliäinen Aurélie Fender Maud Contrant Tomi Ivacevic Vladimir Benes Frédéric Gros Olivier Voinnet Mihaela Zavolan Päivi M Ojala Juergen G Haas Sébastien Pfeffer
author_facet	Guillaume Suffert Georg Malterer Jean Hausser Johanna Viiliäinen Aurélie Fender Maud Contrant Tomi Ivacevic Vladimir Benes Frédéric Gros Olivier Voinnet Mihaela Zavolan Päivi M Ojala Juergen G Haas Sébastien Pfeffer
author_sort	Guillaume Suffert
title	Kaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.
title_short	Kaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.
title_full	Kaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.
title_fullStr	Kaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.
title_full_unstemmed	Kaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.
title_sort	kaposi's sarcoma herpesvirus micrornas target caspase 3 and regulate apoptosis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/3e1319a9b25b4d58809a8612eb7109b1
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Kaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.

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