Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair

Endogenous metals are required for all life, orchestrating the action of diverse cellular processes that are crucial for tissue function. The dynamic wound healing response is underpinned by a plethora of such cellular behaviours, occurring in a time-dependent manner. However, the importance of endo...

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Autores principales:	Holly N. Wilkinson, Barbara-Ann Guinn, Matthew J. Hardman
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	metallome wound healing skin RNA-sequencing calcium metals Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3e1ec66e61da4aec9b2c5202d7465c82
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spelling	oai:doaj.org-article:3e1ec66e61da4aec9b2c5202d7465c822021-12-01T18:04:19ZCombined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair2296-634X10.3389/fcell.2021.788596https://doaj.org/article/3e1ec66e61da4aec9b2c5202d7465c822021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.788596/fullhttps://doaj.org/toc/2296-634XEndogenous metals are required for all life, orchestrating the action of diverse cellular processes that are crucial for tissue function. The dynamic wound healing response is underpinned by a plethora of such cellular behaviours, occurring in a time-dependent manner. However, the importance of endogenous metals for cutaneous repair remains largely unexplored. Here we combine ICP-MS with tissue-level RNA-sequencing to reveal profound changes in a number of metals, and corresponding metal-regulated genes, across temporal healing in mice. Wound calcium, magnesium, iron, copper and manganese are elevated at 7 days post-wounding, while magnesium, iron, aluminium, manganese and cobalt increase at 14 days post-wounding. At the level of transcription, wound-induced pathways are independently highly enriched for metal-regulated genes, and vice versa. Moreover, specific metals are linked to distinct wound-induced biological processes and converge on key transcriptional regulators in mice and humans. Finally, we reveal a potential role for one newly identified transcriptional regulator, TNF, in calcium-induced epidermal differentiation. Together, these data highlight potential new and diverse roles for metals in cutaneous wound repair, paving the way for further studies to elucidate the contribution of metals to cellular processes in the repair of skin and other tissues.Holly N. WilkinsonBarbara-Ann GuinnMatthew J. HardmanFrontiers Media S.A.articlemetallomewound healingskinRNA-sequencingcalciummetalsBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	metallome wound healing skin RNA-sequencing calcium metals Biology (General) QH301-705.5
spellingShingle	metallome wound healing skin RNA-sequencing calcium metals Biology (General) QH301-705.5 Holly N. Wilkinson Barbara-Ann Guinn Matthew J. Hardman Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair
description	Endogenous metals are required for all life, orchestrating the action of diverse cellular processes that are crucial for tissue function. The dynamic wound healing response is underpinned by a plethora of such cellular behaviours, occurring in a time-dependent manner. However, the importance of endogenous metals for cutaneous repair remains largely unexplored. Here we combine ICP-MS with tissue-level RNA-sequencing to reveal profound changes in a number of metals, and corresponding metal-regulated genes, across temporal healing in mice. Wound calcium, magnesium, iron, copper and manganese are elevated at 7 days post-wounding, while magnesium, iron, aluminium, manganese and cobalt increase at 14 days post-wounding. At the level of transcription, wound-induced pathways are independently highly enriched for metal-regulated genes, and vice versa. Moreover, specific metals are linked to distinct wound-induced biological processes and converge on key transcriptional regulators in mice and humans. Finally, we reveal a potential role for one newly identified transcriptional regulator, TNF, in calcium-induced epidermal differentiation. Together, these data highlight potential new and diverse roles for metals in cutaneous wound repair, paving the way for further studies to elucidate the contribution of metals to cellular processes in the repair of skin and other tissues.
format	article
author	Holly N. Wilkinson Barbara-Ann Guinn Matthew J. Hardman
author_facet	Holly N. Wilkinson Barbara-Ann Guinn Matthew J. Hardman
author_sort	Holly N. Wilkinson
title	Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair
title_short	Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair
title_full	Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair
title_fullStr	Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair
title_full_unstemmed	Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair
title_sort	combined metallomics/transcriptomics profiling reveals a major role for metals in wound repair
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3e1ec66e61da4aec9b2c5202d7465c82
work_keys_str_mv	AT hollynwilkinson combinedmetallomicstranscriptomicsprofilingrevealsamajorroleformetalsinwoundrepair AT barbaraannguinn combinedmetallomicstranscriptomicsprofilingrevealsamajorroleformetalsinwoundrepair AT matthewjhardman combinedmetallomicstranscriptomicsprofilingrevealsamajorroleformetalsinwoundrepair
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Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair

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