Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau

The microbial-mediated functional potentials of soil can be reflected by the abundance of corresponding microbial functional genes (MFGs). Few studies have simultaneously examined the responses of multiple key MFGs involving soil carbon (C) and nitrogen (N) transformation to N deposition, particular...

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Autores principales:	Yang Hu, Hongmao Jiang, Youchao Chen, Ziwei Wang, Yan Yan, Ping Sun, Xuyang Lu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Alpine steppe Nitrogen addition Microbial functional genes (MFGs) Q-PCR Tibetan Plateau Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/3e28191ad71c436a8679aacca8375ff2
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spelling	oai:doaj.org-article:3e28191ad71c436a8679aacca8375ff22021-12-02T05:01:48ZNitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau2351-989410.1016/j.gecco.2021.e01937https://doaj.org/article/3e28191ad71c436a8679aacca8375ff22021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S235198942100487Xhttps://doaj.org/toc/2351-9894The microbial-mediated functional potentials of soil can be reflected by the abundance of corresponding microbial functional genes (MFGs). Few studies have simultaneously examined the responses of multiple key MFGs involving soil carbon (C) and nitrogen (N) transformation to N deposition, particularly under multiple application levels. In this study, we treated the alpine soil from the Tibetan Plateau with N fertilization at six addition rates. Absolute quantitative analysis was used to detect the abundance of MFGs related to soil C process: carbon dioxide (CO2) fixation(cbbL), methane (CH4) oxidation (pmoA) and production (mcrA), and soil N process: ammonia oxidation (AOA-amoA: archaea amoA, AOB-amoA: bacterial amoA), hydroxylamine oxidation (hao), nitrate, nitrite, nitric oxide and nitrous oxide (N2O) reduction (narG/napA, nirS/nirK, norB, and nosZ). The abundance of MFGs involved in both the soil C and N transformation processes were reduced as N rates increased (except AOB-amoA gene). Specifically, the abundance of the mcrA, AOA-amoA, nirK, norB, and nosZ genes were decreased non-linearly (U-shape) with the increasing N rates (P < 0.05). The soil pH and dissolved organic C (DOC) concentration were positively correlated with the abundance of those MFGs, suggesting that the decreases in abundance of MFGs were more likely to be driven by changes of soil pH and DOC concentration. In contrast, the abundance of AOB-amoA gene increased linearly with the increasing N rates, which was strongly and positively correlated with soil nitrate-N (NO3–-N) concentration (P < 0.001, r = 0.82). In addition, soil greenhouse gases emissions linked with the abundance of corresponding MFGs. Specifically, the soil CO2 and N2O emissions were strongly and positively correlated with the AOB-amoA gene abundance (P < 0.001, r = 0.78, r = 0.84), and the soil CH4 emissions were significantly correlated with the pmoA (P < 0.01, r = 0.60) and AOA-amoA (P < 0.001, r = 0.72) gene abundance. The above results showed that the AOB-amoA gene had a positive response to increasing N rates, which was different from other genes. Indicating that elevated N deposition may improve the ammonia-oxidation process in alpine steppe soil mediated by AOB communities, and AOB communities may play an important role in donating the soil-atmosphere C and N exchange via CO2 and N2O emissions.Yang HuHongmao JiangYouchao ChenZiwei WangYan YanPing SunXuyang LuElsevierarticleAlpine steppeNitrogen additionMicrobial functional genes (MFGs)Q-PCRTibetan PlateauEcologyQH540-549.5ENGlobal Ecology and Conservation, Vol 32, Iss , Pp e01937- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Alpine steppe Nitrogen addition Microbial functional genes (MFGs) Q-PCR Tibetan Plateau Ecology QH540-549.5
spellingShingle	Alpine steppe Nitrogen addition Microbial functional genes (MFGs) Q-PCR Tibetan Plateau Ecology QH540-549.5 Yang Hu Hongmao Jiang Youchao Chen Ziwei Wang Yan Yan Ping Sun Xuyang Lu Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau
description	The microbial-mediated functional potentials of soil can be reflected by the abundance of corresponding microbial functional genes (MFGs). Few studies have simultaneously examined the responses of multiple key MFGs involving soil carbon (C) and nitrogen (N) transformation to N deposition, particularly under multiple application levels. In this study, we treated the alpine soil from the Tibetan Plateau with N fertilization at six addition rates. Absolute quantitative analysis was used to detect the abundance of MFGs related to soil C process: carbon dioxide (CO2) fixation(cbbL), methane (CH4) oxidation (pmoA) and production (mcrA), and soil N process: ammonia oxidation (AOA-amoA: archaea amoA, AOB-amoA: bacterial amoA), hydroxylamine oxidation (hao), nitrate, nitrite, nitric oxide and nitrous oxide (N2O) reduction (narG/napA, nirS/nirK, norB, and nosZ). The abundance of MFGs involved in both the soil C and N transformation processes were reduced as N rates increased (except AOB-amoA gene). Specifically, the abundance of the mcrA, AOA-amoA, nirK, norB, and nosZ genes were decreased non-linearly (U-shape) with the increasing N rates (P < 0.05). The soil pH and dissolved organic C (DOC) concentration were positively correlated with the abundance of those MFGs, suggesting that the decreases in abundance of MFGs were more likely to be driven by changes of soil pH and DOC concentration. In contrast, the abundance of AOB-amoA gene increased linearly with the increasing N rates, which was strongly and positively correlated with soil nitrate-N (NO3–-N) concentration (P < 0.001, r = 0.82). In addition, soil greenhouse gases emissions linked with the abundance of corresponding MFGs. Specifically, the soil CO2 and N2O emissions were strongly and positively correlated with the AOB-amoA gene abundance (P < 0.001, r = 0.78, r = 0.84), and the soil CH4 emissions were significantly correlated with the pmoA (P < 0.01, r = 0.60) and AOA-amoA (P < 0.001, r = 0.72) gene abundance. The above results showed that the AOB-amoA gene had a positive response to increasing N rates, which was different from other genes. Indicating that elevated N deposition may improve the ammonia-oxidation process in alpine steppe soil mediated by AOB communities, and AOB communities may play an important role in donating the soil-atmosphere C and N exchange via CO2 and N2O emissions.
format	article
author	Yang Hu Hongmao Jiang Youchao Chen Ziwei Wang Yan Yan Ping Sun Xuyang Lu
author_facet	Yang Hu Hongmao Jiang Youchao Chen Ziwei Wang Yan Yan Ping Sun Xuyang Lu
author_sort	Yang Hu
title	Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau
title_short	Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau
title_full	Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau
title_fullStr	Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau
title_full_unstemmed	Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau
title_sort	nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the tibetan plateau
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/3e28191ad71c436a8679aacca8375ff2
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Nitrogen addition altered the microbial functional potentials of carbon and nitrogen transformation in alpine steppe soils on the Tibetan Plateau

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