Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing

The bone marrow microenvironment is composed primarily of immune and stromal cells that play important roles in fracture healing. Although immune cells have been identified in mouse bone marrow, variations in their numbers and type during the fracture healing process remain poorly defined. In this s...

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Autores principales:	Hao Zhang, Renkai Wang, Guangchao Wang, Bo Zhang, Chao Wang, Di Li, Chen Ding, Qiang Wei, Zhenyu Fan, Hao Tang, Fang Ji
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	scRNA-seq fracture healing B cells exosome bone marrow Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/3e366478333b4317a8aa2e15863cb676
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spelling	oai:doaj.org-article:3e366478333b4317a8aa2e15863cb6762021-11-08T06:53:47ZSingle-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing1664-239210.3389/fendo.2021.666140https://doaj.org/article/3e366478333b4317a8aa2e15863cb6762021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fendo.2021.666140/fullhttps://doaj.org/toc/1664-2392The bone marrow microenvironment is composed primarily of immune and stromal cells that play important roles in fracture healing. Although immune cells have been identified in mouse bone marrow, variations in their numbers and type during the fracture healing process remain poorly defined. In this study, single-cell RNA sequencing was used to identify immune cells in fracture tissues, including neutrophils, monocytes, T cells, B cells, and plasma cells. The number of B cells decreased significantly in the early stage of fracture healing. Furthermore, B cells in mice fracture models decreased significantly during the epiphyseal phase and then gradually returned to normal during the epiphyseal transformation phase of fracture healing. The B-cell pattern was opposite to that of bone formation and resorption activities. Notably, B-cell–derived exosomes inhibited bone homeostasis in fracture healing. In humans, a decrease in the number of B cells during the epiphyseal phase stimulated fracture healing. Then, as the numbers of osteoblasts increased during the callus reconstruction stage, the number of B cells gradually recovered, which reduced additional bone regeneration. Thus, B cells are key regulators of fracture healing and inhibit excessive bone regeneration by producing multiple osteoblast inhibitors.Hao ZhangRenkai WangRenkai WangGuangchao WangBo ZhangChao WangDi LiChen DingQiang WeiZhenyu FanHao TangFang JiFang JiFrontiers Media S.A.articlescRNA-seqfracture healingB cellsexosomebone marrowDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENFrontiers in Endocrinology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	scRNA-seq fracture healing B cells exosome bone marrow Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	scRNA-seq fracture healing B cells exosome bone marrow Diseases of the endocrine glands. Clinical endocrinology RC648-665 Hao Zhang Renkai Wang Renkai Wang Guangchao Wang Bo Zhang Chao Wang Di Li Chen Ding Qiang Wei Zhenyu Fan Hao Tang Fang Ji Fang Ji Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing
description	The bone marrow microenvironment is composed primarily of immune and stromal cells that play important roles in fracture healing. Although immune cells have been identified in mouse bone marrow, variations in their numbers and type during the fracture healing process remain poorly defined. In this study, single-cell RNA sequencing was used to identify immune cells in fracture tissues, including neutrophils, monocytes, T cells, B cells, and plasma cells. The number of B cells decreased significantly in the early stage of fracture healing. Furthermore, B cells in mice fracture models decreased significantly during the epiphyseal phase and then gradually returned to normal during the epiphyseal transformation phase of fracture healing. The B-cell pattern was opposite to that of bone formation and resorption activities. Notably, B-cell–derived exosomes inhibited bone homeostasis in fracture healing. In humans, a decrease in the number of B cells during the epiphyseal phase stimulated fracture healing. Then, as the numbers of osteoblasts increased during the callus reconstruction stage, the number of B cells gradually recovered, which reduced additional bone regeneration. Thus, B cells are key regulators of fracture healing and inhibit excessive bone regeneration by producing multiple osteoblast inhibitors.
format	article
author	Hao Zhang Renkai Wang Renkai Wang Guangchao Wang Bo Zhang Chao Wang Di Li Chen Ding Qiang Wei Zhenyu Fan Hao Tang Fang Ji Fang Ji
author_facet	Hao Zhang Renkai Wang Renkai Wang Guangchao Wang Bo Zhang Chao Wang Di Li Chen Ding Qiang Wei Zhenyu Fan Hao Tang Fang Ji Fang Ji
author_sort	Hao Zhang
title	Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing
title_short	Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing
title_full	Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing
title_fullStr	Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing
title_full_unstemmed	Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing
title_sort	single-cell rna sequencing reveals b cells are important regulators in fracture healing
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3e366478333b4317a8aa2e15863cb676
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Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing

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