Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia

Abstract. Susanti R, Yuniastuti A. 2020. Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia. Biodiversitas 21: 605-610. Genetic conservation is an effective approach to preserve unique and protected species in Indonesia, including indigenous d...

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Autores principales:	R Susanti, Ari Yuniastuti
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	prolactin, x-collagen, duck, pcr-rflp Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3e3d3d670a8e4b768a121d5801947310
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spelling	oai:doaj.org-article:3e3d3d670a8e4b768a121d58019473102021-11-21T22:01:10ZShort Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia1412-033X2085-472210.13057/biodiv/d210223https://doaj.org/article/3e3d3d670a8e4b768a121d58019473102020-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4986https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Susanti R, Yuniastuti A. 2020. Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia. Biodiversitas 21: 605-610. Genetic conservation is an effective approach to preserve unique and protected species in Indonesia, including indigenous duck (Anas platyrhynchos Linnaeus, 1758). One of the most important things is an identification effort by studying genetic characteristics to support genetic resource conservation programs. This study aim was to analyze the genetic polymorphisms of Central Javanese local duck based on prolactin (PRL) and collagen-x (COLX) genes. A total of 35 local ducks in Central Java was collected as samples, the duck’s calamus feathers were taken for tissue DNA isolation. Polymorphism analysis of PRL and COLX genes were performed using single nucleotide polymorphism (SNP) markers and PCR-RFLP method. The amplicons were then digested with DraI restriction enzyme (AhaIII) (5'TTT/ AAA) for PRL gene, and HaeIII (BsuRI) enzyme (5'GG/ CC-3 ') for COLX gene. The genotype was determined based on RFLP PCR amplicon band results from each target gene. For each gene, data on genotype frequencies, allele frequencies, expected heterozygosities (HE), and Hardy-Weinberg equilibrium were analyzed using PopGene32 software. Based on the PRL gene, there were 25 ducks (71.43%) with BB genotype and 10 ducks (28.57%) with AB genotype. While based on the COLX gene, as many as 25 ducks (71.43%) had TT genotypes, 9 ducks (25.71%) had TC types and 1 duck (1.86%) had CC types. Both PRL and COLX genotypes were clustered the Central Javanese local ducks into 5 haplotypes (A-E). The high allele frequency has belonged to B allele (85.71%) of the PRL gene and T allele (84.25%) of the COLX gene that showed high egg production and quality. Allele B (PRL gene) and T allele (COLX gene) could be predicted as weight eggs’ alleles marker.R SusantiAri YuniastutiMBI & UNS Soloarticleprolactin, x-collagen, duck, pcr-rflpBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	prolactin, x-collagen, duck, pcr-rflp Biology (General) QH301-705.5
spellingShingle	prolactin, x-collagen, duck, pcr-rflp Biology (General) QH301-705.5 R Susanti Ari Yuniastuti Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia
description	Abstract. Susanti R, Yuniastuti A. 2020. Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia. Biodiversitas 21: 605-610. Genetic conservation is an effective approach to preserve unique and protected species in Indonesia, including indigenous duck (Anas platyrhynchos Linnaeus, 1758). One of the most important things is an identification effort by studying genetic characteristics to support genetic resource conservation programs. This study aim was to analyze the genetic polymorphisms of Central Javanese local duck based on prolactin (PRL) and collagen-x (COLX) genes. A total of 35 local ducks in Central Java was collected as samples, the duck’s calamus feathers were taken for tissue DNA isolation. Polymorphism analysis of PRL and COLX genes were performed using single nucleotide polymorphism (SNP) markers and PCR-RFLP method. The amplicons were then digested with DraI restriction enzyme (AhaIII) (5'TTT/ AAA) for PRL gene, and HaeIII (BsuRI) enzyme (5'GG/ CC-3 ') for COLX gene. The genotype was determined based on RFLP PCR amplicon band results from each target gene. For each gene, data on genotype frequencies, allele frequencies, expected heterozygosities (HE), and Hardy-Weinberg equilibrium were analyzed using PopGene32 software. Based on the PRL gene, there were 25 ducks (71.43%) with BB genotype and 10 ducks (28.57%) with AB genotype. While based on the COLX gene, as many as 25 ducks (71.43%) had TT genotypes, 9 ducks (25.71%) had TC types and 1 duck (1.86%) had CC types. Both PRL and COLX genotypes were clustered the Central Javanese local ducks into 5 haplotypes (A-E). The high allele frequency has belonged to B allele (85.71%) of the PRL gene and T allele (84.25%) of the COLX gene that showed high egg production and quality. Allele B (PRL gene) and T allele (COLX gene) could be predicted as weight eggs’ alleles marker.
format	article
author	R Susanti Ari Yuniastuti
author_facet	R Susanti Ari Yuniastuti
author_sort	R Susanti
title	Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia
title_short	Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia
title_full	Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia
title_fullStr	Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia
title_full_unstemmed	Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia
title_sort	short communication: prolactin and x-collagen genes polymorphism in central javanese local ducks, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/3e3d3d670a8e4b768a121d5801947310
work_keys_str_mv	AT rsusanti shortcommunicationprolactinandxcollagengenespolymorphismincentraljavaneselocalducksindonesia AT ariyuniastuti shortcommunicationprolactinandxcollagengenespolymorphismincentraljavaneselocalducksindonesia
_version_	1718418576647389184

Short Communication: Prolactin and x-collagen genes polymorphism in Central Javanese local ducks, Indonesia

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