Outer hair cells isolation from postnatal Sprague–Dawley rats

Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss. In order to elucidate the mechanism underlying these disorders, OHCs – its diseased region needs to be deeply investigated. However, OHCs array on the basila...

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Autores principales:	Mei-Hao Qi, Yang Qiu, Ke-Yong Tian, Kun Liang, Hui-Min Chang, Ren-Feng Wang, Er-Fang Chen, Wei-Long Wang, Ding-Jun Zha, Jian-Hua Qiu
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2019
Materias:	Otorhinolaryngology RF1-547 Surgery RD1-811
Acceso en línea:	https://doaj.org/article/3e4d0540a98a4d9886145856ee6bb2c4
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spelling	oai:doaj.org-article:3e4d0540a98a4d9886145856ee6bb2c42021-12-02T12:53:46ZOuter hair cells isolation from postnatal Sprague–Dawley rats2095-881110.1016/j.wjorl.2018.01.001https://doaj.org/article/3e4d0540a98a4d9886145856ee6bb2c42019-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2095881117300641https://doaj.org/toc/2095-8811Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss. In order to elucidate the mechanism underlying these disorders, OHCs – its diseased region needs to be deeply investigated. However, OHCs array on the basilar membrane which contains massive cells with different types. Therefore, to isolate OHCs from this huge population is significant for revealing its pathological and molecular changes during disease processing. In the present study, we tried to isolate OHCs from the commonly used animal model –Sprague-Dawley (SD) rats. By separating outer hair cells from SD rats with different day ages, we found that 9 days after birth was a suitable time for the separation of the OHCs. At this time, the number of OHCs isolated from rats was large, and the cell morphology was typical of cylindrical shape. OHCs isolated using this method are histologically suitable and quantitatively adequate for molecular biological and electrophysiological analyses. Keywords: Outer hair cell, Isolation technique, Morphology, Sprague–Dawley ratMei-Hao QiYang QiuKe-Yong TianKun LiangHui-Min ChangRen-Feng WangEr-Fang ChenWei-Long WangDing-Jun ZhaJian-Hua QiuKeAi Communications Co., Ltd.articleOtorhinolaryngologyRF1-547SurgeryRD1-811ENWorld Journal of Otorhinolaryngology-Head and Neck Surgery, Vol 5, Iss 1, Pp 14-18 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Otorhinolaryngology RF1-547 Surgery RD1-811
spellingShingle	Otorhinolaryngology RF1-547 Surgery RD1-811 Mei-Hao Qi Yang Qiu Ke-Yong Tian Kun Liang Hui-Min Chang Ren-Feng Wang Er-Fang Chen Wei-Long Wang Ding-Jun Zha Jian-Hua Qiu Outer hair cells isolation from postnatal Sprague–Dawley rats
description	Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss. In order to elucidate the mechanism underlying these disorders, OHCs – its diseased region needs to be deeply investigated. However, OHCs array on the basilar membrane which contains massive cells with different types. Therefore, to isolate OHCs from this huge population is significant for revealing its pathological and molecular changes during disease processing. In the present study, we tried to isolate OHCs from the commonly used animal model –Sprague-Dawley (SD) rats. By separating outer hair cells from SD rats with different day ages, we found that 9 days after birth was a suitable time for the separation of the OHCs. At this time, the number of OHCs isolated from rats was large, and the cell morphology was typical of cylindrical shape. OHCs isolated using this method are histologically suitable and quantitatively adequate for molecular biological and electrophysiological analyses. Keywords: Outer hair cell, Isolation technique, Morphology, Sprague–Dawley rat
format	article
author	Mei-Hao Qi Yang Qiu Ke-Yong Tian Kun Liang Hui-Min Chang Ren-Feng Wang Er-Fang Chen Wei-Long Wang Ding-Jun Zha Jian-Hua Qiu
author_facet	Mei-Hao Qi Yang Qiu Ke-Yong Tian Kun Liang Hui-Min Chang Ren-Feng Wang Er-Fang Chen Wei-Long Wang Ding-Jun Zha Jian-Hua Qiu
author_sort	Mei-Hao Qi
title	Outer hair cells isolation from postnatal Sprague–Dawley rats
title_short	Outer hair cells isolation from postnatal Sprague–Dawley rats
title_full	Outer hair cells isolation from postnatal Sprague–Dawley rats
title_fullStr	Outer hair cells isolation from postnatal Sprague–Dawley rats
title_full_unstemmed	Outer hair cells isolation from postnatal Sprague–Dawley rats
title_sort	outer hair cells isolation from postnatal sprague–dawley rats
publisher	KeAi Communications Co., Ltd.
publishDate	2019
url	https://doaj.org/article/3e4d0540a98a4d9886145856ee6bb2c4
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Outer hair cells isolation from postnatal Sprague–Dawley rats

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