Vitamin D induces increased systolic arterial pressure via vascular reactivity and mechanical properties.

<h4>Background/aims</h4>The aim of this study was to evaluate whether supplementation of high doses of cholecalciferol for two months in normotensive rats results in increased systolic arterial pressure and which are the mechanisms involved. Specifically, this study assesses the potentia...

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Autores principales:	Priscila Portugal Dos Santos, Bruna Paola Murino Rafacho, Andréa de Freitas Gonçalves, Rodrigo Gibin Jaldin, Thiago Bruder do Nascimento, Marcondes Alves Barbosa Silva, Stêfany Bruno Assis Cau, Meliza Goi Roscani, Paula Schimdt Azevedo, Marcos Ferreira Minicucci, Rita de Cássia Tostes, Leonardo Antonio Memede Zornoff, Sergio Alberto Rupp de Paiva
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3e54af42d42f4b3fa98f9752335163bd
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spelling	oai:doaj.org-article:3e54af42d42f4b3fa98f9752335163bd2021-11-18T08:16:00ZVitamin D induces increased systolic arterial pressure via vascular reactivity and mechanical properties.1932-620310.1371/journal.pone.0098895https://doaj.org/article/3e54af42d42f4b3fa98f9752335163bd2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24921930/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background/aims</h4>The aim of this study was to evaluate whether supplementation of high doses of cholecalciferol for two months in normotensive rats results in increased systolic arterial pressure and which are the mechanisms involved. Specifically, this study assesses the potential effect on cardiac output as well as the changes in aortic structure and functional properties.<h4>Methods</h4>Male Wistar rats were divided into three groups: 1) Control group (C, n = 20), with no supplementation of vitamin D, 2) VD3 (n = 19), supplemented with 3,000 IU vitamin D/kg of chow; 3) VD10 (n = 21), supplemented with 10,000 IU vitamin D/kg of chow. After two months, echocardiographic analyses, measurements of systolic arterial pressure (SAP), vascular reactivity, reactive oxygen species (ROS) generation, mechanical properties, histological analysis and metalloproteinase-2 and -9 activity were performed.<h4>Results</h4>SAP was higher in VD3 and VD10 than in C rats (p = 0.001). Echocardiographic variables were not different among groups. Responses to phenylephrine in endothelium-denuded aortas was higher in VD3 compared to the C group (p = 0.041). Vascular relaxation induced by acetylcholine (p = 0.023) and sodium nitroprusside (p = 0.005) was impaired in both supplemented groups compared to the C group and apocynin treatment reversed impaired vasodilation. Collagen volume fraction (<0.001) and MMP-2 activity (p = 0.025) was higher in VD10 group compared to the VD3 group. Elastin volume fraction was lower in VD10 than in C and yield point was lower in VD3 than in C.<h4>Conclusion</h4>Our findings support the view that vitamin D supplementation increases arterial pressure in normotensive rats and this is associated with structural and functional vascular changes, modulated by NADPH oxidase, nitric oxide, and extracellular matrix components.Priscila Portugal Dos SantosBruna Paola Murino RafachoAndréa de Freitas GonçalvesRodrigo Gibin JaldinThiago Bruder do NascimentoMarcondes Alves Barbosa SilvaStêfany Bruno Assis CauMeliza Goi RoscaniPaula Schimdt AzevedoMarcos Ferreira MinicucciRita de Cássia TostesLeonardo Antonio Memede ZornoffSergio Alberto Rupp de PaivaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98895 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Priscila Portugal Dos Santos Bruna Paola Murino Rafacho Andréa de Freitas Gonçalves Rodrigo Gibin Jaldin Thiago Bruder do Nascimento Marcondes Alves Barbosa Silva Stêfany Bruno Assis Cau Meliza Goi Roscani Paula Schimdt Azevedo Marcos Ferreira Minicucci Rita de Cássia Tostes Leonardo Antonio Memede Zornoff Sergio Alberto Rupp de Paiva Vitamin D induces increased systolic arterial pressure via vascular reactivity and mechanical properties.
description	<h4>Background/aims</h4>The aim of this study was to evaluate whether supplementation of high doses of cholecalciferol for two months in normotensive rats results in increased systolic arterial pressure and which are the mechanisms involved. Specifically, this study assesses the potential effect on cardiac output as well as the changes in aortic structure and functional properties.<h4>Methods</h4>Male Wistar rats were divided into three groups: 1) Control group (C, n = 20), with no supplementation of vitamin D, 2) VD3 (n = 19), supplemented with 3,000 IU vitamin D/kg of chow; 3) VD10 (n = 21), supplemented with 10,000 IU vitamin D/kg of chow. After two months, echocardiographic analyses, measurements of systolic arterial pressure (SAP), vascular reactivity, reactive oxygen species (ROS) generation, mechanical properties, histological analysis and metalloproteinase-2 and -9 activity were performed.<h4>Results</h4>SAP was higher in VD3 and VD10 than in C rats (p = 0.001). Echocardiographic variables were not different among groups. Responses to phenylephrine in endothelium-denuded aortas was higher in VD3 compared to the C group (p = 0.041). Vascular relaxation induced by acetylcholine (p = 0.023) and sodium nitroprusside (p = 0.005) was impaired in both supplemented groups compared to the C group and apocynin treatment reversed impaired vasodilation. Collagen volume fraction (<0.001) and MMP-2 activity (p = 0.025) was higher in VD10 group compared to the VD3 group. Elastin volume fraction was lower in VD10 than in C and yield point was lower in VD3 than in C.<h4>Conclusion</h4>Our findings support the view that vitamin D supplementation increases arterial pressure in normotensive rats and this is associated with structural and functional vascular changes, modulated by NADPH oxidase, nitric oxide, and extracellular matrix components.
format	article
author	Priscila Portugal Dos Santos Bruna Paola Murino Rafacho Andréa de Freitas Gonçalves Rodrigo Gibin Jaldin Thiago Bruder do Nascimento Marcondes Alves Barbosa Silva Stêfany Bruno Assis Cau Meliza Goi Roscani Paula Schimdt Azevedo Marcos Ferreira Minicucci Rita de Cássia Tostes Leonardo Antonio Memede Zornoff Sergio Alberto Rupp de Paiva
author_facet	Priscila Portugal Dos Santos Bruna Paola Murino Rafacho Andréa de Freitas Gonçalves Rodrigo Gibin Jaldin Thiago Bruder do Nascimento Marcondes Alves Barbosa Silva Stêfany Bruno Assis Cau Meliza Goi Roscani Paula Schimdt Azevedo Marcos Ferreira Minicucci Rita de Cássia Tostes Leonardo Antonio Memede Zornoff Sergio Alberto Rupp de Paiva
author_sort	Priscila Portugal Dos Santos
title	Vitamin D induces increased systolic arterial pressure via vascular reactivity and mechanical properties.
title_short	Vitamin D induces increased systolic arterial pressure via vascular reactivity and mechanical properties.
title_full	Vitamin D induces increased systolic arterial pressure via vascular reactivity and mechanical properties.
title_fullStr	Vitamin D induces increased systolic arterial pressure via vascular reactivity and mechanical properties.
title_full_unstemmed	Vitamin D induces increased systolic arterial pressure via vascular reactivity and mechanical properties.
title_sort	vitamin d induces increased systolic arterial pressure via vascular reactivity and mechanical properties.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3e54af42d42f4b3fa98f9752335163bd
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Vitamin D induces increased systolic arterial pressure via vascular reactivity and mechanical properties.

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