Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model

The administration of cardiosphere-derived cells (CDCs) after acute myocardial infarction (AMI) is very promising. CDC encapsulation in alginate-poly-<span style="font-variant: small-caps;">l</span>-lysine-alginate (APA) could increase cell survival and adherence. The intraperi...

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Autores principales:	Claudia Báez-Díaz, Virginia Blanco-Blázquez, Francisco Miguel Sánchez-Margallo, Esther López, Helena Martín, Albert Espona-Noguera, Javier G. Casado, Jesús Ciriza, José Luis Pedraz, Verónica Crisóstomo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	CDCs AMI intrapericardial microcapsules APA swine Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/3e561752daf44aaa95fd92ae106f53c2
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spelling	oai:doaj.org-article:3e561752daf44aaa95fd92ae106f53c22021-11-25T18:40:55ZIntrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model10.3390/pharmaceutics131118241999-4923https://doaj.org/article/3e561752daf44aaa95fd92ae106f53c22021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1824https://doaj.org/toc/1999-4923The administration of cardiosphere-derived cells (CDCs) after acute myocardial infarction (AMI) is very promising. CDC encapsulation in alginate-poly-<span style="font-variant: small-caps;">l</span>-lysine-alginate (APA) could increase cell survival and adherence. The intrapericardial (IP) approach potentially achieves high concentrations of the therapeutic agent in the infarcted area. We aimed to evaluate IP therapy using a saline vehicle as a control (CON), a dose of 30 × 10<sup>6</sup> CDCs (CDCs) or APA microcapsules containing 30 × 10<sup>6</sup> CDCs (APA-CDCs) at 72 h in a porcine AMI model. Magnetic resonance imaging (MRI) was used to determine the left ventricular ejection fraction (LVEF), infarct size (IS), and indexed end diastolic and systolic volumes (EDVi; ESVi) pre- and 10 weeks post-injection. Programmed electrical stimulation (PES) was performed to test arrhythmia inducibility before euthanasia. Histopathological analysis was carried out afterwards. The IP infusion was successful in all animals. At 10 weeks, MRI revealed significantly higher LVEF in the APA-CDC group compared with CON. No significant differences were observed among groups in IS, EDVi, ESVi, PES and histopathological analyses. In conclusion, the IP injection of CDCs (microencapsulated or not) was feasible and safe 72 h post-AMI in the porcine model. Moreover, CDCs APA encapsulation could have a beneficial effect on cardiac function, reflected by a higher LVEF at 10 weeks.Claudia Báez-DíazVirginia Blanco-BlázquezFrancisco Miguel Sánchez-MargalloEsther LópezHelena MartínAlbert Espona-NogueraJavier G. CasadoJesús CirizaJosé Luis PedrazVerónica CrisóstomoMDPI AGarticleCDCsAMIintrapericardialmicrocapsulesAPAswinePharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1824, p 1824 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CDCs AMI intrapericardial microcapsules APA swine Pharmacy and materia medica RS1-441
spellingShingle	CDCs AMI intrapericardial microcapsules APA swine Pharmacy and materia medica RS1-441 Claudia Báez-Díaz Virginia Blanco-Blázquez Francisco Miguel Sánchez-Margallo Esther López Helena Martín Albert Espona-Noguera Javier G. Casado Jesús Ciriza José Luis Pedraz Verónica Crisóstomo Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model
description	The administration of cardiosphere-derived cells (CDCs) after acute myocardial infarction (AMI) is very promising. CDC encapsulation in alginate-poly-<span style="font-variant: small-caps;">l</span>-lysine-alginate (APA) could increase cell survival and adherence. The intrapericardial (IP) approach potentially achieves high concentrations of the therapeutic agent in the infarcted area. We aimed to evaluate IP therapy using a saline vehicle as a control (CON), a dose of 30 × 10<sup>6</sup> CDCs (CDCs) or APA microcapsules containing 30 × 10<sup>6</sup> CDCs (APA-CDCs) at 72 h in a porcine AMI model. Magnetic resonance imaging (MRI) was used to determine the left ventricular ejection fraction (LVEF), infarct size (IS), and indexed end diastolic and systolic volumes (EDVi; ESVi) pre- and 10 weeks post-injection. Programmed electrical stimulation (PES) was performed to test arrhythmia inducibility before euthanasia. Histopathological analysis was carried out afterwards. The IP infusion was successful in all animals. At 10 weeks, MRI revealed significantly higher LVEF in the APA-CDC group compared with CON. No significant differences were observed among groups in IS, EDVi, ESVi, PES and histopathological analyses. In conclusion, the IP injection of CDCs (microencapsulated or not) was feasible and safe 72 h post-AMI in the porcine model. Moreover, CDCs APA encapsulation could have a beneficial effect on cardiac function, reflected by a higher LVEF at 10 weeks.
format	article
author	Claudia Báez-Díaz Virginia Blanco-Blázquez Francisco Miguel Sánchez-Margallo Esther López Helena Martín Albert Espona-Noguera Javier G. Casado Jesús Ciriza José Luis Pedraz Verónica Crisóstomo
author_facet	Claudia Báez-Díaz Virginia Blanco-Blázquez Francisco Miguel Sánchez-Margallo Esther López Helena Martín Albert Espona-Noguera Javier G. Casado Jesús Ciriza José Luis Pedraz Verónica Crisóstomo
author_sort	Claudia Báez-Díaz
title	Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model
title_short	Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model
title_full	Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model
title_fullStr	Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model
title_full_unstemmed	Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model
title_sort	intrapericardial delivery of apa-microcapsules as promising stem cell therapy carriers in an experimental acute myocardial infarction model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3e561752daf44aaa95fd92ae106f53c2
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Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model

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