Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy

Li He,1,* Fangzhen Qing,2,* Maode Li,3 Daitian Lan3 1Department of Thyroid and Breast Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital (East Hospital), Chengdu 610100, Sichuan, People’s Republic of China; 2Department of Stomatology, Sich...

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Autores principales:	He L, Qing F, Li M, Lan D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	ir1061 second near infrared window paclitaxel apoferritin “all-in-one” nanoplatform Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3e5c7a486ab24a458fe877813d06f3cf
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spelling	oai:doaj.org-article:3e5c7a486ab24a458fe877813d06f3cf2021-12-02T11:05:02ZPaclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy1178-2013https://doaj.org/article/3e5c7a486ab24a458fe877813d06f3cf2020-04-01T00:00:00Zhttps://www.dovepress.com/paclitaxelir1061-co-loaded-protein-nanoparticle-for-tumor-targeted-and-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Li He,1,* Fangzhen Qing,2,* Maode Li,3 Daitian Lan3 1Department of Thyroid and Breast Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital (East Hospital), Chengdu 610100, Sichuan, People’s Republic of China; 2Department of Stomatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital (East Hospital), Chengdu 610100, Sichuan, People’s Republic of China; 3Department of Hepatobiliary and Pancreatic Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital (East Hospital), Chengdu 610100, Sichuan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Daitian LanDepartment of Hepatobiliary and Pancreatic Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital (East Hospital), Chengdu 610100, Sichuan, People’s Republic of ChinaTel +86-18349176311Email doctor_yinl@126.comPurpose: The aim of this study was to develop an “all-in-one” nanoplatform that integrates at the second near-infrared (NIR-II) region dye IR1061 and anticancer drug paclitaxel (PTX) into an apoferritin (AFN) nanocage (IR-AFN@PTX). Simultaneously, folic acid (FA), tumor target molecule,  was conjugated onto IR-AFN@PTX to be IR-AFN@PTX-FA for tumor-targeted and pH/NIR-II-triggered synergistic photothermal-chemotherapy.Methods: IR1061 was firstly reacted with PEG and then conjugated with AFN to be IR-AFN. Then, FA was conjugated onto the surface of IR-AFN to be IR-AFN-FA. At last, PTX was incorporated into IR-AFN-FA to fabricate a nanoplatform IR-AFN@PTX-FA. The NIR-II photothermal properties and pH/NIR-II triggered drug release were evaluated. The ability of IR-AFN@PTX-FA to target tumors was estimated using optical bioluminescence. In vitro and in vivo synergistic therapeutic effects of pH/NIR-II-triggered and tumor-targeted photothermal-chemotherapy were investigated in 4T1 tumor model.Results: IR-AFN@PTX-FA showed excellent water solubility and physiological stability, which significantly enhanced the solubility of both IR1061 and PTX. After 5 min of laser irradiation at 1064 nm, IR-AFN@PTX-FA exhibited an effective photothermal effect compared with laser irradiation at 808 nm, even when blocked with 0.6 cm thick chicken breast. Cellular uptake experiments showed IR-AFN@PTX-FA utilized clathrin-mediated and caveolae-mediated endocytosis pathways to enter 4T1 cells, and was then delivered by the endosome to the lysosome. NIR-II laser irradiation and pH could synergistically trigger PTX release, inducing significant tumor inhibition in vitro and in vivo.Conclusion: As a novel “all-in-one” nanoplatform, IR-AFN@PTX-FA was found to selectively target tumors and showed very efficient NIR-II photothermal effects and pH/NIR-II triggered drug release effects, showing a remarkable, synergistic photothermal-chemotherapy effect.Keywords: IR1061, second near-infrared window, paclitaxel, apoferritin, “all-in-one” nanoplatformHe LQing FLi MLan DDove Medical Pressarticleir1061second near infrared windowpaclitaxelapoferritin“all-in-one” nanoplatformMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2337-2349 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	ir1061 second near infrared window paclitaxel apoferritin “all-in-one” nanoplatform Medicine (General) R5-920
spellingShingle	ir1061 second near infrared window paclitaxel apoferritin “all-in-one” nanoplatform Medicine (General) R5-920 He L Qing F Li M Lan D Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy
description	Li He,1,* Fangzhen Qing,2,* Maode Li,3 Daitian Lan3 1Department of Thyroid and Breast Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital (East Hospital), Chengdu 610100, Sichuan, People’s Republic of China; 2Department of Stomatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital (East Hospital), Chengdu 610100, Sichuan, People’s Republic of China; 3Department of Hepatobiliary and Pancreatic Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital (East Hospital), Chengdu 610100, Sichuan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Daitian LanDepartment of Hepatobiliary and Pancreatic Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital (East Hospital), Chengdu 610100, Sichuan, People’s Republic of ChinaTel +86-18349176311Email doctor_yinl@126.comPurpose: The aim of this study was to develop an “all-in-one” nanoplatform that integrates at the second near-infrared (NIR-II) region dye IR1061 and anticancer drug paclitaxel (PTX) into an apoferritin (AFN) nanocage (IR-AFN@PTX). Simultaneously, folic acid (FA), tumor target molecule,  was conjugated onto IR-AFN@PTX to be IR-AFN@PTX-FA for tumor-targeted and pH/NIR-II-triggered synergistic photothermal-chemotherapy.Methods: IR1061 was firstly reacted with PEG and then conjugated with AFN to be IR-AFN. Then, FA was conjugated onto the surface of IR-AFN to be IR-AFN-FA. At last, PTX was incorporated into IR-AFN-FA to fabricate a nanoplatform IR-AFN@PTX-FA. The NIR-II photothermal properties and pH/NIR-II triggered drug release were evaluated. The ability of IR-AFN@PTX-FA to target tumors was estimated using optical bioluminescence. In vitro and in vivo synergistic therapeutic effects of pH/NIR-II-triggered and tumor-targeted photothermal-chemotherapy were investigated in 4T1 tumor model.Results: IR-AFN@PTX-FA showed excellent water solubility and physiological stability, which significantly enhanced the solubility of both IR1061 and PTX. After 5 min of laser irradiation at 1064 nm, IR-AFN@PTX-FA exhibited an effective photothermal effect compared with laser irradiation at 808 nm, even when blocked with 0.6 cm thick chicken breast. Cellular uptake experiments showed IR-AFN@PTX-FA utilized clathrin-mediated and caveolae-mediated endocytosis pathways to enter 4T1 cells, and was then delivered by the endosome to the lysosome. NIR-II laser irradiation and pH could synergistically trigger PTX release, inducing significant tumor inhibition in vitro and in vivo.Conclusion: As a novel “all-in-one” nanoplatform, IR-AFN@PTX-FA was found to selectively target tumors and showed very efficient NIR-II photothermal effects and pH/NIR-II triggered drug release effects, showing a remarkable, synergistic photothermal-chemotherapy effect.Keywords: IR1061, second near-infrared window, paclitaxel, apoferritin, “all-in-one” nanoplatform
format	article
author	He L Qing F Li M Lan D
author_facet	He L Qing F Li M Lan D
author_sort	He L
title	Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy
title_short	Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy
title_full	Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy
title_fullStr	Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy
title_full_unstemmed	Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy
title_sort	paclitaxel/ir1061-co-loaded protein nanoparticle for tumor-targeted and ph/nir-ii-triggered synergistic photothermal-chemotherapy
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/3e5c7a486ab24a458fe877813d06f3cf
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Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy

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