In vivo MRI evaluation of early postnatal development in normal and impaired rat eyes

Abstract This study employed in vivo 7-T magnetic resonance imaging (MRI) to evaluate the postnatal ocular growth patterns under normal development or neonatal impairments in Sprague–Dawley rats. Using T2-weighted imaging on healthy rats from postnatal day (P) 1 (newborn) to P60 (adult), the volumes...

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Autores principales:	Jeannie M. Au, Swarupa Kancherla, Malack Hamade, Monica Mendoza, Kevin C. Chan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3e5f47fddfbb451ba55004abe4ef9df0
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spelling	oai:doaj.org-article:3e5f47fddfbb451ba55004abe4ef9df02021-12-02T16:06:43ZIn vivo MRI evaluation of early postnatal development in normal and impaired rat eyes10.1038/s41598-021-93991-22045-2322https://doaj.org/article/3e5f47fddfbb451ba55004abe4ef9df02021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93991-2https://doaj.org/toc/2045-2322Abstract This study employed in vivo 7-T magnetic resonance imaging (MRI) to evaluate the postnatal ocular growth patterns under normal development or neonatal impairments in Sprague–Dawley rats. Using T2-weighted imaging on healthy rats from postnatal day (P) 1 (newborn) to P60 (adult), the volumes of the anterior chamber and posterior chamber (ACPC), lens, and vitreous humor increased logistically with ACPC expanding by 33-fold and the others by fivefold. Intravitreal potassium dichromate injection at P1, P7, and P14 led to T1-weighted signal enhancement in the developing retina by 188–289%. Upon unilateral hypoxic-ischemic encephalopathy at P7, monocular deprivation at P15, and monocular enucleation at P1, T2-weighted imaging of the adult rats showed decreased ocular volumes to different extents. In summary, in vivo high-field MRI allows for non-invasive evaluation of early postnatal development in the normal and impaired rat eyes. Chromium-enhanced MRI appeared effective in examining the developing retina before natural eyelid opening at P14 with relevance to lipid metabolism. The reduced ocular volumes upon neonatal visual impairments provided evidence to the emerging problems of why some impaired visual outcomes cannot be solely predicted by neurological assessments and suggested the need to look into both the eye and the brain under such conditions.Jeannie M. AuSwarupa KancherlaMalack HamadeMonica MendozaKevin C. ChanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeannie M. Au Swarupa Kancherla Malack Hamade Monica Mendoza Kevin C. Chan In vivo MRI evaluation of early postnatal development in normal and impaired rat eyes
description	Abstract This study employed in vivo 7-T magnetic resonance imaging (MRI) to evaluate the postnatal ocular growth patterns under normal development or neonatal impairments in Sprague–Dawley rats. Using T2-weighted imaging on healthy rats from postnatal day (P) 1 (newborn) to P60 (adult), the volumes of the anterior chamber and posterior chamber (ACPC), lens, and vitreous humor increased logistically with ACPC expanding by 33-fold and the others by fivefold. Intravitreal potassium dichromate injection at P1, P7, and P14 led to T1-weighted signal enhancement in the developing retina by 188–289%. Upon unilateral hypoxic-ischemic encephalopathy at P7, monocular deprivation at P15, and monocular enucleation at P1, T2-weighted imaging of the adult rats showed decreased ocular volumes to different extents. In summary, in vivo high-field MRI allows for non-invasive evaluation of early postnatal development in the normal and impaired rat eyes. Chromium-enhanced MRI appeared effective in examining the developing retina before natural eyelid opening at P14 with relevance to lipid metabolism. The reduced ocular volumes upon neonatal visual impairments provided evidence to the emerging problems of why some impaired visual outcomes cannot be solely predicted by neurological assessments and suggested the need to look into both the eye and the brain under such conditions.
format	article
author	Jeannie M. Au Swarupa Kancherla Malack Hamade Monica Mendoza Kevin C. Chan
author_facet	Jeannie M. Au Swarupa Kancherla Malack Hamade Monica Mendoza Kevin C. Chan
author_sort	Jeannie M. Au
title	In vivo MRI evaluation of early postnatal development in normal and impaired rat eyes
title_short	In vivo MRI evaluation of early postnatal development in normal and impaired rat eyes
title_full	In vivo MRI evaluation of early postnatal development in normal and impaired rat eyes
title_fullStr	In vivo MRI evaluation of early postnatal development in normal and impaired rat eyes
title_full_unstemmed	In vivo MRI evaluation of early postnatal development in normal and impaired rat eyes
title_sort	in vivo mri evaluation of early postnatal development in normal and impaired rat eyes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3e5f47fddfbb451ba55004abe4ef9df0
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In vivo MRI evaluation of early postnatal development in normal and impaired rat eyes

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